Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,865	T→C	A388A (GCA→GCG)	tldD ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,865	0	T	C	100.0%	150.6 / NA	42	A388A (GCA→GCG)	tldD	putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/12);  total (30/12)

ATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGTGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAACGC  >  NC_000913/3390725‑3391012                                                                                                                                             |                                                                                                                                                    aTGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATAc                                                                                                                                           >  1:564608/1‑151 (MQ=255)  tGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATAcc                                                                                                                                          >  1:655064/1‑151 (MQ=255)    gTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGt                                                                                                                                        >  1:522233/1‑151 (MQ=255)              cGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACgg                                                                                                                               >  1:730660/1‑150 (MQ=255)              cGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACgg                                                                                                                               >  2:170639/1‑150 (MQ=255)                gAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGAtt                                                                                                                            <  1:166891/151‑1 (MQ=255)                   ccAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCa                                                                                                                          >  2:64311/1‑150 (MQ=255)                    cAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaat                                                                                                                        <  1:749371/151‑1 (MQ=255)                       tCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaataat                                                                                                                     >  1:411612/1‑151 (MQ=255)                       tCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCaataat                                                                                                                     >  1:97841/1‑151 (MQ=255)                            gTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCt                                                                                                                >  2:672663/1‑151 (MQ=255)                              tGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTg                                                                                                               <  1:64312/150‑1 (MQ=255)                              tGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTg                                                                                                               <  1:241162/150‑1 (MQ=255)                               gcgcCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCg                                                                                                             <  2:584140/151‑1 (MQ=255)                                   cTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGt                                                                                                         >  2:728099/1‑151 (MQ=255)                                         ccGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGAttt                                                                                                   >  2:389804/1‑151 (MQ=255)                                          cGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGa                                                                                                      <  1:671657/147‑1 (MQ=255)                                               tCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCgg                                                                                             >  1:581581/1‑151 (MQ=255)                                                 gTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGgc                                                                                            >  1:717534/1‑150 (MQ=255)                                                        tACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCatat                                                                                     >  2:616790/1‑150 (MQ=255)                                                          ccGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAgg                                                                                  >  2:147035/1‑151 (MQ=255)                                                                tCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTgg                                                                            >  1:550866/1‑151 (MQ=255)                                                                 cAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGt                                                                           <  1:133817/151‑1 (MQ=255)                                                                 cAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGt                                                                           >  1:518275/1‑151 (MQ=255)                                                                            gCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGc                                                                 >  1:271661/1‑150 (MQ=255)                                                                            gCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGc                                                                 >  2:484474/1‑150 (MQ=255)                                                                              ttCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATg                                                              >  1:86299/1‑151 (MQ=255)                                                                                cTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATggg                                                            >  1:349853/1‑151 (MQ=255)                                                                                           aaaaCGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGt                                                 <  1:381904/151‑1 (MQ=255)                                                                                                    ttGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCa                                         <  2:161361/150‑1 (MQ=255)                                                                                                         ggAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCg                                    >  2:703624/1‑150 (MQ=255)                                                                                                         ggAGGTGATATCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCg                                    >  1:326772/1‑150 (MQ=255)                                                                                                                 tatCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGcc                           >  1:183224/1‑151 (MQ=255)                                                                                                                   tCCACCTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGcc                           >  1:460030/1‑149 (MQ=255)                                                                                                                        cTGACCGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGc                    >  2:332466/1‑151 (MQ=255)                                                                                                                            ccGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTc                 >  2:418823/1‑150 (MQ=255)                                                                                                                             cGCCACCAAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCAt               <  1:417573/151‑1 (MQ=255)                                                                                                                                   caAAGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCAt         >  1:134573/1‑151 (MQ=255)                                                                                                                                    aaaGTTCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATc        >  2:394284/1‑151 (MQ=255)                                                                                                                                         tCGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAc    <  2:326759/150‑1 (MQ=255)                                                                                                                                          cGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAcgc  >  1:531101/1‑151 (MQ=255)                                                                                                                                          cGGCGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAAcg   <  2:662815/150‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    ATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTTCGGTGCATAGATACCGTACTCAACGGATTCAATAATTTCCTGCGGGGTCGATTTACCCGGCAGCATATAGGTGTTGGTCATACGCGGCATGGGCAGATGGGCGTAGGATTCACGGCGACCGTTGCCAGTCGGCGTCATCCCCATCAAACGC  >  NC_000913/3390725‑3391012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑