Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,635	G→A	R110W (CGG→TGG)	yrdD ←	ssDNA‑binding protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,635	0	G	A	100.0%	177.7 / NA	45	R110W (CGG→TGG)	yrdD	ssDNA‑binding protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/28);  total (17/28)

TTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCC  >  NC_000913/3431487‑3431781                                                                                                                                                     |                                                                                                                                                   ttcttttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAAc                                                                                                                                                  >  2:657045/1‑151 (MQ=255)     tttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTg                                                                                                                                              >  1:555231/1‑151 (MQ=255)      ttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTg                                                                                                                                              <  2:765235/150‑1 (MQ=255)        cGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTggg                                                                                                                                            <  1:521048/150‑1 (MQ=255)            gAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCa                                                                                                                                        <  2:33065/150‑1 (MQ=255)             aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATg                                                                                                                                      <  2:405784/151‑1 (MQ=255)                  cGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAAtt                                                                                                                                 <  1:675767/151‑1 (MQ=255)                    gATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGc                                                                                                                               <  1:593206/151‑1 (MQ=255)                     aTAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCt                                                                                                                              >  1:162564/1‑151 (MQ=255)                       aaTGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGt                                                                                                                            >  2:51200/1‑151 (MQ=255)                                cAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCg                                                                                                                    <  2:534515/150‑1 (MQ=255)                                 aGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGt                                                                                                                  <  2:260501/151‑1 (MQ=255)                                   ggCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGttt                                                                                                                >  1:311464/1‑151 (MQ=255)                                           ccAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGa                                                                                                           <  1:369966/148‑1 (MQ=255)                                              gCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGtt                                                                                                     <  1:240697/151‑1 (MQ=255)                                                       ttGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGt                                                                                             <  2:504207/150‑1 (MQ=255)                                                           aGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGc                                                                                         >  1:72158/1‑150 (MQ=255)                                                           aGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGc                                                                                         >  2:617359/1‑150 (MQ=255)                                                                aTGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAg                                                                                   <  2:473431/151‑1 (MQ=255)                                                                       aTTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGtt                                                                            >  1:332446/1‑151 (MQ=255)                                                                       aTTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGtt                                                                            <  2:478313/151‑1 (MQ=255)                                                                            cacTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATg                                                                        <  2:564121/150‑1 (MQ=255)                                                                                ccGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCaa                                                                     <  2:757518/149‑1 (MQ=255)                                                                                  gggTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCaa                                                                 <  1:614708/151‑1 (MQ=255)                                                                                  gggTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCaa                                                                 <  2:740896/151‑1 (MQ=255)                                                                                        cGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAAc                                                            >  2:635753/1‑150 (MQ=255)                                                                                         gATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACAt                                                          <  2:697448/151‑1 (MQ=255)                                                                                          aTCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATa                                                         <  1:192953/151‑1 (MQ=255)                                                                                               aaGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCTTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAATTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACGaaa                                                    <  2:140389/151‑1 (MQ=255)                                                                                                       aaTGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCt                                            <  1:377422/151‑1 (MQ=255)                                                                                                            tttGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCg                                        <  2:403374/150‑1 (MQ=255)                                                                                                               gCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTaa                                     >  2:194268/1‑150 (MQ=255)                                                                                                                ccATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAAt                                    >  2:660785/1‑150 (MQ=255)                                                                                                                ccATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAACTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAAt                                    >  2:759790/1‑150 (MQ=255)                                                                                                                  aTAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATAcc                                 >  2:576737/1‑151 (MQ=255)                                                                                                                           gcggcgCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTgcg                        <  2:725011/151‑1 (MQ=255)                                                                                                                           gcggcgCTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTgcg                        <  2:74102/151‑1 (MQ=255)                                                                                                                                gcTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCcac                    >  2:102931/1‑150 (MQ=255)                                                                                                                                 cTGGACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACAt                  >  1:747123/1‑151 (MQ=255)                                                                                                                                   ggACCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATg                 <  1:486014/150‑1 (MQ=255)                                                                                                                                     aCCAGATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAg              <  2:376878/151‑1 (MQ=255)                                                                                                                                         gATGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGc           >  1:617150/1‑150 (MQ=255)                                                                                                                                          aTGGCCCGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCaa         <  2:555329/151‑1 (MQ=255)                                                                                                                                               ccGTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTg    >  2:86947/1‑151 (MQ=255)                                                                                                                                                 gTCCAACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGcc  <  2:233120/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCC  >  NC_000913/3431487‑3431781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑