Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,608	T→C	M240V (ATG→GTG)	ugpB ←	sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,608	0	T	C	100.0%	178.5 / NA	49	M240V (ATG→GTG)	ugpB	sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

GCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGC  >  NC_000913/3591462‑3591754                                                                                                                                                   |                                                                                                                                                   gCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACctcc                                                                                                                                                >  1:415817/1‑151 (MQ=255) gCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGAAGCTGAAGTCGCCCTTCTTGTTCACctcc                                                                                                                                                >  1:557180/1‑151 (MQ=255)  ccTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCCcctcct                                                                                                                                               <  2:79338/151‑1 (MQ=255)      cGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCg                                                                                                                                             >  1:337092/1‑149 (MQ=255)        ccGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCa                                                                                                                                         <  2:160078/151‑1 (MQ=255)                  aTTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGAtgtg                                                                                                                               <  2:196155/151‑1 (MQ=255)                     tGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGtt                                                                                                                             <  2:558894/150‑1 (MQ=255)                                cgAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCg                                                                                                                 <  2:19511/151‑1 (MQ=255)                                     gTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCtt                                                                                                             <  2:40402/150‑1 (MQ=255)                                           aaGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGGGGATTCCTCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTTACCTGCTCCGGCTTATTGaa                                                                                                       >  2:696222/1‑150 (MQ=255)                                               gaACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCa                                                                                                  <  2:640696/151‑1 (MQ=255)                                                 aCCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGc                                                                                                <  2:313612/151‑1 (MQ=255)                                                    ggAAGAGGCGGTGGCCATCGCGCAATCAACGTTATAGAAATTCTCGGGGGGTTCATCCTTTAGACCGACGTAGCTGAAAGCGCCCTTCTTGGTTACCTCCTCGCGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTTAACTCCAGCAcc                                                                                             >  2:389634/1‑151 (MQ=255)                                                         agGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTc                                                                                        <  1:525783/151‑1 (MQ=255)                                                          ggCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTcc                                                                                       <  2:530523/151‑1 (MQ=255)                                                                 gtCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGt                                                                                <  1:117029/151‑1 (MQ=255)                                                                 gtCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGt                                                                                <  1:31165/151‑1 (MQ=255)                                                                        cgcAATCACCGTTATAGAACTTCTCGGTAGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCTCGTCCGTGCCGTCaaa                                                                            <  2:461713/148‑1 (MQ=255)                                                                                ccGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGtttt                                                                 >  2:675063/1‑151 (MQ=255)                                                                                  gTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGttttt                                                                >  2:391666/1‑150 (MQ=255)                                                                                   ttATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGc                                                              >  2:319855/1‑151 (MQ=255)                                                                                     ataGCACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGACGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGGTATATGTTGCGCCTGCCCCGGCTTATTAACCTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCt                                                             <  2:323396/150‑1 (MQ=255)                                                                                     ataGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCt                                                             <  1:203560/150‑1 (MQ=255)                                                                                       aGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGc                                                          >  2:658867/1‑151 (MQ=255)                                                                                        gAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCa                                                         <  2:271863/151‑1 (MQ=255)                                                                                         aaCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaa                                                        <  2:714077/151‑1 (MQ=255)                                                                                          aCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaaa                                                       <  2:526900/151‑1 (MQ=255)                                                                                          aCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaa                                                        >  2:478523/1‑150 (MQ=255)                                                                                             tctcGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACg                                                     <  1:594674/150‑1 (MQ=255)                                                                                                cGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACgg                                                    <  2:15966/148‑1 (MQ=255)                                                                                                   tGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGAcc                                              >  1:437535/1‑151 (MQ=255)                                                                                                         cATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTcc                                         >  2:267984/1‑150 (MQ=255)                                                                                                          aTCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTcc                                         >  1:286417/1‑149 (MQ=255)                                                                                                            ccTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgc                                     <  2:724211/151‑1 (MQ=255)                                                                                                                aCGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCt                                  >  2:217666/1‑150 (MQ=255)                                                                                                                   accgacGTAGCTGAAGTCGCTCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTaa                                >  1:286049/1‑149 (MQ=255)                                                                                                                      gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcg                                    >  1:685383/1‑142 (MQ=255)                                                                                                                      gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcg                                    <  2:685341/142‑1 (MQ=255)                                                                                                                      gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGttt                           >  2:511779/1‑151 (MQ=255)                                                                                                                       acGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGtttt                          >  2:304706/1‑151 (MQ=255)                                                                                                                       acGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTAGCAAACGGCAGACCGTTCCAGGCGCTAAAGtttt                          >  2:690804/1‑151 (MQ=255)                                                                                                                        cGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTc                         >  1:100196/1‑151 (MQ=255)                                                                                                                         gTAGCTGAAGACGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTcc                        <  2:624875/151‑1 (MQ=255)                                                                                                                           aGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGTGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCa                       <  1:433044/150‑1 (MQ=255)                                                                                                                           aGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAg                      <  1:235344/151‑1 (MQ=255)                                                                                                                               gAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGtt                    <  1:4975/149‑1 (MQ=255)                                                                                                                                    cGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCa             <  2:309667/151‑1 (MQ=255)                                                                                                                                     gCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATcc              >  2:291539/1‑149 (MQ=255)                                                                                                                                              gTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCa    <  1:172712/150‑1 (MQ=255)                                                                                                                                               ttCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGc  >  2:381213/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGC  >  NC_000913/3591462‑3591754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑