Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,592,873	T→C	T189A (ACT→GCT)	livF ←	branched‑chain amino acid ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,592,873	0	T	C	100.0%	171.3 / NA	45	T189A (ACT→GCT)	livF	branched‑chain amino acid ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/9);  total (36/9)

CTAAATACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCC  >  NC_000913/3592732‑3593020                                                                                                                                              |                                                                                                                                                    cTAAATACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTATCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAACGATAGCCATCCCCt                                                                                                                                             >  2:361905/1‑150 (MQ=255)    aaTACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGCGAAAGATAGCCATCCCCTGCTc                                                                                                                                         >  2:277564/1‑151 (MQ=255)          cACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCgcgc                                                                                                                                     >  2:173581/1‑149 (MQ=255)          cACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCgcgc                                                                                                                                     >  1:591217/1‑149 (MQ=255)                 cACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTc                                                                                                                             <  2:300990/150‑1 (MQ=255)                  aCCGCTTAATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGAACCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTAGCGCAGCTGCTCg                                                                                                                            >  2:359704/1‑150 (MQ=255)                      cTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                                                                                       >  2:135445/1‑151 (MQ=255)                        tCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                                                                                       <  2:647411/149‑1 (MQ=255)                          aTTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCg                                                                                                                   >  2:161973/1‑151 (MQ=255)                                  gcagcGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                                                                                           >  1:451098/1‑151 (MQ=255)                                         aTCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                                                                                    >  1:613721/1‑151 (MQ=255)                                            aCCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatgat                                                                                                  >  1:301372/1‑150 (MQ=255)                                                       aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCa                                                                                        >  1:719482/1‑149 (MQ=255)                                                         aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                                                                                    <  1:223469/151‑1 (MQ=255)                                                            aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCg                                                                                  >  2:184601/1‑150 (MQ=255)                                                                 aTGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                                                                            >  2:193500/1‑151 (MQ=255)                                                                    gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGATTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGc                                                                         >  1:631946/1‑151 (MQ=255)                                                                       gTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtc                                                                       >  2:452693/1‑150 (MQ=255)                                                                             cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc                                                                >  1:139825/1‑151 (MQ=255)                                                                             cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc                                                                <  1:432371/151‑1 (MQ=255)                                                                                cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGt                                                             >  1:601888/1‑151 (MQ=255)                                                                                cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGt                                                             >  1:295500/1‑151 (MQ=255)                                                                                 aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTa                                                            <  1:452714/151‑1 (MQ=255)                                                                                  cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGt                                                             >  2:401101/1‑149 (MQ=255)                                                                                   gTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGc                                                          >  1:672348/1‑151 (MQ=255)                                                                                         gcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAAcgc                                                    >  1:605179/1‑151 (MQ=255)                                                                                           gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCgg                                                  >  2:50017/1‑151 (MQ=255)                                                                                                      tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCtcatc                                        >  2:569935/1‑150 (MQ=255)                                                                                                      tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCtcatc                                        >  2:309037/1‑150 (MQ=255)                                                                                                         aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAgcg                                    <  2:118609/151‑1 (MQ=255)                                                                                                         aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAgcg                                    <  2:357000/151‑1 (MQ=255)                                                                                                           cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAgcgc                                   >  1:423224/1‑150 (MQ=255)                                                                                                            gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCAc                                 >  1:708093/1‑151 (MQ=255)                                                                                                                ggTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGAcc                             >  2:523317/1‑151 (MQ=255)                                                                                                                ggTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGAcc                             >  2:516984/1‑151 (MQ=255)                                                                                                                ggTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGAcc                             >  1:368331/1‑151 (MQ=255)                                                                                                                 gTTGGCGTTCTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGAcc                             >  1:756926/1‑150 (MQ=255)                                                                                                                    ggCGTTCTGCTCGACGAGAAAGGTAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATc                         <  1:110381/151‑1 (MQ=255)                                                                                                                          cTGCTCGACGAGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGc                   >  1:590813/1‑151 (MQ=255)                                                                                                                                gacgaGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATctgc             >  1:8426/1‑151 (MQ=255)                                                                                                                                gacgaGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATctgc             >  2:566275/1‑151 (MQ=255)                                                                                                                                gacgaGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATctgc             >  1:718682/1‑151 (MQ=255)                                                                                                                                  cgaGAAAGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATctgctg           >  2:439137/1‑151 (MQ=255)                                                                                                                                      aaaGATAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCa       <  1:681451/151‑1 (MQ=255)                                                                                                                                           tAGCCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCAccgcc  >  1:624123/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    CTAAATACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCC  >  NC_000913/3592732‑3593020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑