Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,619,262 C→T A24V (GCC→GTC)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,619,2620CT95.0% 43.8 / ‑6.1 21A24V (GCC→GTC) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/3);  minor base G (1/0);  total (18/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGATACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTC  >  NC_000913/3619123‑3619407
                                                                                                                                           |                                                                                                                                                 
gACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGATACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGGACg                                                                                                                                          >  1:518231/1‑149 (MQ=255)
              cttcGCTTTTTCGTATGAAGATACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCAcc                                                                                                                          >  2:391761/1‑151 (MQ=255)
                         cGTATGAAGATACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGGACGTATTGGTGCCCCCACCGGCGTGGcc                                                                                                                 >  1:556731/1‑149 (MQ=255)
                                               aataatAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTATCATTGTTCAGGGGTCAGTCGGGGTACGGATTGGGTCCCCCACCGGCGTGGCCTGTTGCGCGTGGCCccgcggtg                                                                                           >  2:408411/1‑147 (MQ=17)
                                                    taGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCCTTGTTCCGGGTTCAGGCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGGGTGCCCCGGGGGGGTggc                                                                                       >  2:407987/1‑146 (MQ=17)
                                                           ggATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCAt                                                                             <  2:556703/151‑1 (MQ=255)
                                                                 tACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTc                                                                       >  1:625941/1‑151 (MQ=255)
                                                                   cGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGTCGGGGGACGTATTTGTGCCCCCACCGGCGTTGCCCGTTCGGGGTGGCCCGGCGGAGTGCCGTCCCGCCCTcc                                                                           >  2:576948/1‑145 (MQ=14)
                                                                    gATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGTCGGGGGACGTATTGGTGCCCCCACCGGCGTGGGCTGTTCGGTGTGCCCCGGCGGAGGGACGTCCGGCCATCCGGTCaa                                                                     >  2:103269/1‑150 (MQ=255)
                                                                        aGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGGGACGTCCGGCCATCCGGTCAATcccc                                                                >  1:556291/1‑151 (MQ=255)
                                                                                                         aCGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGTCGGGGGACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGGGACGTCCCGCCATCCGGGCAATCCCCTGGTCGGGGCAAAGGGCCCTCCCGGGGAAAcc                                >  2:402685/1‑150 (MQ=11)
                                                                                                             aGTATGGCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACAt                           <  1:317974/151‑1 (MQ=18)
                                                                                                                   gCGGTAGCATTGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCt                     >  1:430797/1‑151 (MQ=18)
                                                                                                                             tGTTCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgcccggcccg           >  2:275781/1‑151 (MQ=18)
                                                                                                                               ttCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTg        >  1:633328/1‑151 (MQ=18)
                                                                                                                                tCAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTg        >  1:361633/1‑151 (MQ=18)
                                                                                                                                tCAGGGTTCAGACGGGGTACGGATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGGCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTTCTCGGTGGAAAGGACCTTCCCGGTGAAACCGACATCACCCTGCCCAGCCCGCt         >  2:255620/1‑150 (MQ=11)
                                                                                                                                 cAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTg        <  2:472232/150‑1 (MQ=18)
                                                                                                                                 cAGGGTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGc       >  2:558141/1‑151 (MQ=18)
                                                                                                                                    ggTTCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGt    >  2:668109/1‑151 (MQ=18)
                                                                                                                                       tCAGTCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTc  >  2:607437/1‑150 (MQ=18)
                                                                                                                                           |                                                                                                                                                 
GACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGATACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTC  >  NC_000913/3619123‑3619407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: