Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,455	T→C	S1088S (AGT→AGC)	rhsB →	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,455	0	T	C	100.0%	114.1 / NA	52	S1088S (AGT→AGC)	rhsB	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

GACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3622309‑3622567                                                                                                                                                   |                                                                                                                 gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCgtgg                                                                                                              >  1:614918/1‑151 (MQ=255)        gCCGCGTCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCGCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTccc                                                                                                       <  2:658708/151‑1 (MQ=255)            cATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCccgccg                                                                                                   >  2:632121/1‑151 (MQ=25)                 aGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCt                                                                                              >  1:242269/1‑151 (MQ=25)                     gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  1:218982/1‑151 (MQ=25)                     gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  2:547961/1‑151 (MQ=25)                     gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                          >  2:627008/1‑151 (MQ=25)                     gATTTACCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCCCCGGTGAGCGGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGCCGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGt                                                                                           <  2:256313/150‑1 (MQ=255)                       tttACCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAACCTGCCACCGGGGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                         <  1:397522/150‑1 (MQ=255)                             aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGc                                                                                   >  1:260584/1‑150 (MQ=25)                             aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  >  1:202509/1‑151 (MQ=25)                             aGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  >  1:232847/1‑151 (MQ=25)                              gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  1:364138/150‑1 (MQ=25)                              gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  2:126795/150‑1 (MQ=25)                              gCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTc                                                                                 >  2:129450/1‑151 (MQ=25)                                 ggggAGCTTCACGCCCCTCATCAGGGTCGAAGCTGCCACGGGTGAGCTGGCGAAAACGCGGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGGGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCg                                                                                <  2:420710/149‑1 (MQ=11)                                 ggggAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGa                                                                               >  2:54567/1‑150 (MQ=25)                                 ggggAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGCGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCCCGAc                                                                              >  2:570944/1‑151 (MQ=255)                                 ggggAGCTGCACGCCACTCACCGGGGTCGAACCTGCCACCGGGGAGCTGGCAAAACCGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                  <  2:723640/147‑1 (MQ=11)                                       cTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                        >  2:159635/1‑151 (MQ=25)                                         tCACGCCACTCATCAGGGTCGAACCTGCCGCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCGGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                        <  2:121002/149‑1 (MQ=255)                                           aCGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaa                                                                     >  2:184020/1‑150 (MQ=25)                                             gCCACTCATCAGGGTCGAAACTGCCCCCGTTGAGCTGGCGAAAACGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCCGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                       <  2:207633/146‑1 (MQ=255)                                             gCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAg                                                                   >  2:334070/1‑150 (MQ=25)                                               cACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                 >  1:446609/1‑150 (MQ=25)                                               cACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGa                                                                <  1:508813/151‑1 (MQ=25)                                               cACTCAGCGGGGTCGAAACTGCCACCGGTGAGCTGGCGAAGACGCAGCGCCGCGGCCTGGCGGTTGCCTTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGa                                                                <  2:167500/151‑1 (MQ=11)                                                   catcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATc                                                            >  2:729317/1‑151 (MQ=25)                                                   catcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATc                                                            <  1:262095/151‑1 (MQ=25)                                                               acctccccccGGTGAGCTGGCGAAAACGCAGCGCCGAAGCCTGGCGGATGCCCTTCCGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:355894/143‑1 (MQ=11)                                                               aaCTGCCCCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGCGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                <  1:727834/151‑1 (MQ=255)                                                                  tGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGAGAACAGGGGGGCAGCGAGGTGGTCCCGCGGGTGCTGGAGCGGATGCTCGGCAGGCTGGAGAGTGAAATGCTGGCTGGCCGGgtg                                             >  2:238773/1‑151 (MQ=11)                                                                   gCCCCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             <  2:597740/150‑1 (MQ=14)                                                                   gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgt                                              <  2:296080/149‑1 (MQ=14)                                                                    ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                           <  1:728879/151‑1 (MQ=14)                                                                    ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                             >  2:627771/1‑149 (MQ=14)                                                                     cACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                           >  2:441783/1‑150 (MQ=14)                                                                      aCCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgagt                                          >  2:732629/1‑150 (MQ=14)                                                                           tGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGa                                     >  2:678338/1‑150 (MQ=14)                                                                              gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                 <  1:736397/151‑1 (MQ=14)                                                                                 ggCGAAAACGCGGCGCCGCGGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgcc                                <  2:738914/149‑1 (MQ=9)                                                                                 ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgccg                               <  2:159167/150‑1 (MQ=14)                                                                                      aaaCGCAGCGCCGCGCCCGGGCGGATGCCCTTCAGCAGTCCGGCGGGGAAGAGGGTGGCGGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                         <  2:615513/151‑1 (MQ=11)                                                                                       aaCGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctgg                        >  1:327010/1‑151 (MQ=14)                                                                                          gCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                     >  1:73417/1‑151 (MQ=14)                                                                                            aGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                     <  1:129453/149‑1 (MQ=14)                                                                                                ccgcAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATc                >  2:391754/1‑150 (MQ=14)                                                                                                     gCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCg           <  1:378535/150‑1 (MQ=14)                                                                                                         gggggATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg      <  1:465601/148‑1 (MQ=14)                                                                                                         ggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCGGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt       <  2:368647/150‑1 (MQ=14)                                                                                                         ggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt       <  1:632158/150‑1 (MQ=14)                                                                                                              aTGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCg  >  2:573038/1‑150 (MQ=14)                                                                                                                                                   |                                                                                                                 GACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCG  >  NC_000913/3622309‑3622567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑