Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,715,662	T→C	T245A (ACC→GCC)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,715,662	0	T	C	100.0%	220.1 / NA	61	T245A (ACC→GCC)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/43);  total (18/43)

GGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGCATT  >  NC_000913/3715516‑3715803                                                                                                                                                   |                                                                                                                                              ggcggTTTTACCTATTCCGTCACTCTAGCCCAGGAAATAAGAGGCGAAGACGGCATAACCTGTGGCGCAACGCGCCAGAAACGCTTCGTCGCGCCAACCATTTTCCACCCGCGTATGGGCGATCCCCAGCCCCAGCGCAACATCGGCGccc                                                                                                                                           >  2:259728/1‑151 (MQ=255)  gcggTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCa                                                                                                                                          <  1:69879/151‑1 (MQ=255)    ggTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCAtg                                                                                                                                        >  1:125861/1‑151 (MQ=255)       tttCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGc                                                                                                                                     >  1:294049/1‑151 (MQ=255)         tCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCgg                                                                                                                                   >  1:295785/1‑151 (MQ=255)         tCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCg                                                                                                                                    <  1:184900/150‑1 (MQ=255)          cGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGt                                                                                                                                  >  2:225488/1‑151 (MQ=255)           gCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTg                                                                                                                                 <  2:503411/151‑1 (MQ=255)           gCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTg                                                                                                                                 <  1:412868/151‑1 (MQ=255)              aTTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCCCCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGcca                                                                                                                              <  1:183008/151‑1 (MQ=255)                tCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGccacc                                                                                                                            >  2:87330/1‑151 (MQ=255)                 ccGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGccacc                                                                                                                            <  1:407043/150‑1 (MQ=255)                     cACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTc                                                                                                                       >  1:630843/1‑151 (MQ=255)                      aCTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTcc                                                                                                                      <  2:463674/151‑1 (MQ=255)                         ctcGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCa                                                                                                                     <  1:427674/149‑1 (MQ=255)                           cGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCAtttt                                                                                                                 <  2:145184/151‑1 (MQ=255)                           cGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCAttt                                                                                                                  <  2:653996/150‑1 (MQ=255)                           cGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCAt                                                                                                                    <  2:515482/148‑1 (MQ=255)                           cGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCAt                                                                                                                    <  2:497156/148‑1 (MQ=255)                             cccAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTAt                                                                                                               <  2:56277/151‑1 (MQ=255)                                   aaaTAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCaa                                                                                                         <  2:70535/151‑1 (MQ=255)                                       aaGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGaa                                                                                                     <  2:25930/151‑1 (MQ=255)                                        agagGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGaaa                                                                                                    <  2:666227/151‑1 (MQ=255)                                          agGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATc                                                                                                  <  1:679550/151‑1 (MQ=255)                                             cGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGAc                                                                                               <  2:57096/151‑1 (MQ=255)                                               aaGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACgg                                                                                             >  1:650191/1‑151 (MQ=255)                                                   cGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTc                                                                                         >  1:721412/1‑151 (MQ=255)                                                   cGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTc                                                                                         <  1:293904/151‑1 (MQ=255)                                                         aaCCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCg                                                                                   >  1:146068/1‑151 (MQ=255)                                                          aCCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGc                                                                                  >  2:567199/1‑151 (MQ=255)                                                          aCCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGc                                                                                  <  2:191717/151‑1 (MQ=255)                                                              gtggtgCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCAtt                                                                               <  2:187737/150‑1 (MQ=255)                                                                ggtgCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTgg                                                                             <  1:161115/150‑1 (MQ=255)                                                                ggtgCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGa                                                                            <  2:683785/151‑1 (MQ=255)                                                                     aaCGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAAt                                                                       <  2:249921/151‑1 (MQ=255)                                                                     aaCGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAAt                                                                       >  1:752981/1‑151 (MQ=255)                                                                              gAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCa                                                               <  1:112055/150‑1 (MQ=255)                                                                               aaaCGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGc                                                             <  2:112220/151‑1 (MQ=255)                                                                                  cGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCttt                                                          >  1:53980/1‑151 (MQ=255)                                                                                      tcgtcgTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCttttt                                                        <  2:720530/149‑1 (MQ=255)                                                                                      tcgtcgTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTc                                                       <  1:503837/150‑1 (MQ=255)                                                                                        gtcgtGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCg                                                    <  1:34396/151‑1 (MQ=255)                                                                                           gtGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCt                                                  <  2:468774/150‑1 (MQ=255)                                                                                             gCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTc                                               <  2:718160/151‑1 (MQ=255)                                                                                             gCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTc                                               <  2:318277/151‑1 (MQ=255)                                                                                                  ccATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCa                                          <  1:385659/151‑1 (MQ=255)                                                                                                      tttCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTg                                       <  1:86409/150‑1 (MQ=255)                                                                                                         ccaccaGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAg                                    <  2:473733/150‑1 (MQ=255)                                                                                                          caccaGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGaa                                  <  2:726292/151‑1 (MQ=255)                                                                                                               gCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTa                             <  2:514183/151‑1 (MQ=255)                                                                                                               gCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTa                             <  1:14738/151‑1 (MQ=255)                                                                                                                 gTATGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTa                             >  2:213126/1‑149 (MQ=255)                                                                                                                   aTGGGCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGa                          <  2:746056/150‑1 (MQ=255)                                                                                                                     gggCGATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAg                       <  1:665378/151‑1 (MQ=255)                                                                                                                         gATCCCCAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGcccc                   >  2:231346/1‑151 (MQ=255)                                                                                                                             cccAGCATCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAACGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCt               >  2:723788/1‑151 (MQ=255)                                                                                                                                   aTCAGCGCAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCgg         >  2:723956/1‑151 (MQ=255)                                                                                                                                       gcgcAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGc     <  2:440625/151‑1 (MQ=255)                                                                                                                                        cgcAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGCa    <  1:271152/151‑1 (MQ=255)                                                                                                                                        cgcAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGCa    <  1:225912/151‑1 (MQ=255)                                                                                                                                          cAACATCGGCGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGCAtt  >  1:103383/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                              GGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTCCATTTTATCGCCAAAGAAATCGACGGTTTCCGATCGCATTGGATCAATGCAGATCAGCTTTTTCCCGCTGTCACGCAGTGCAGAAAAGTAAGAAAGCCCCTGCTCATCGGATGCATT  >  NC_000913/3715516‑3715803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑