Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,811,334	A→G	R28R (CGT→CGC)	rpmG ←	50S ribosomal subunit protein L33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,811,334	0	A	G	100.0%	171.0 / NA	47	R28R (CGT→CGC)	rpmG	50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

GGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGAA  >  NC_000913/3811190‑3811475                                                                                                                                                 |                                                                                                                                              ggCAAGCAGATAACAAAAAGCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGtt                                                                                                                                           <  1:110848/149‑1 (MQ=255)     aGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGttct                                                                                                                                     <  2:754476/151‑1 (MQ=255)          aTAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGt                                                                                                                                 >  1:268335/1‑150 (MQ=255)                aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGt                                                                                                                           >  2:188080/1‑150 (MQ=255)                     ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGa                                                                                                                      >  2:46955/1‑150 (MQ=255)                      cccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGa                                                                                                                      >  2:255403/1‑149 (MQ=255)                               gCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGt                                                                                                            >  2:67104/1‑150 (MQ=255)                                 gAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTCTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTAcc                                                                                                         >  2:309817/1‑151 (MQ=255)                                              aCATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAc                                                                                             <  2:634544/150‑1 (MQ=255)                                               cATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAcct                                                                                           <  2:474708/151‑2 (MQ=255)                                               cATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAAcc                                                                                            >  1:678633/1‑150 (MQ=255)                                                aTCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAg                                                                                          <  2:442989/151‑1 (MQ=255)                                                  cAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCt                                                                                        >  2:707285/1‑151 (MQ=255)                                                  cAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCt                                                                                        >  2:185720/1‑151 (MQ=255)                                                   aaaGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCtt                                                                                       >  1:765986/1‑151 (MQ=255)                                                     aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:289509/151‑1 (MQ=255)                                                     aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:140842/151‑1 (MQ=255)                                                     aGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGa                                                                                     <  2:170083/151‑1 (MQ=255)                                                           aaTTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTtct                                                                               <  2:408490/151‑1 (MQ=255)                                                              tattTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCAc                                                                            >  2:2718/1‑151 (MQ=255)                                                               attTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCAc                                                                            <  1:687186/150‑1 (MQ=255)                                                                 ttGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGa                                                                          <  2:110127/150‑1 (MQ=255)                                                                    aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      >  2:12656/1‑151 (MQ=255)                                                                    aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      <  2:646063/151‑1 (MQ=255)                                                                    aTTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATAc                                                                      >  2:161678/1‑151 (MQ=255)                                                                      tttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCt                                                                    >  2:342901/1‑151 (MQ=255)                                                                       ttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCtt                                                                   >  1:726636/1‑151 (MQ=255)                                                                       ttCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCt                                                                    <  1:669193/150‑1 (MQ=255)                                                                         cGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCttt                                                                  <  2:765939/150‑1 (MQ=255)                                                                         cGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTa                                                                 <  2:30815/151‑1 (MQ=255)                                                                          gCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCttt                                                                  >  1:592604/1‑149 (MQ=255)                                                                             tcttTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGcc                                                              <  1:337950/150‑1 (MQ=255)                                                                                        cACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTc                                                  <  1:46956/151‑1 (MQ=255)                                                                                           gTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCctc                                               >  1:331921/1‑151 (MQ=255)                                                                                             gCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCctct                                              <  2:476651/150‑1 (MQ=255)                                                                                             gCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTa                                             <  2:669213/151‑1 (MQ=255)                                                                                                ggCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagt                                          >  2:25386/1‑151 (MQ=255)                                                                                                 gCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagta                                         <  1:547772/151‑1 (MQ=255)                                                                                                         cTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAagtacttagtac                                 >  1:422326/1‑151 (MQ=255)                                                                                                                gAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGc                          <  2:419354/151‑1 (MQ=255)                                                                                                                 aaTTTTTTCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGc                          >  2:509713/1‑150 (MQ=255)                                                                                                                        tCAGTTCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGc                   <  1:262025/150‑1 (MQ=255)                                                                                                                             tCCAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACGTTTCGCCACGGGCACGCa              <  2:508252/150‑1 (MQ=255)                                                                                                                               cAGTTTTTCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGtt           >  1:661380/1‑151 (MQ=255)                                                                                                                                   ttttCCGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGc       >  1:89879/1‑151 (MQ=255)                                                                                                                                        cGGCTTAGTGCGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGa   >  1:359060/1‑150 (MQ=255)                                                                                                                                         gtcTTAGTGCGTTTGTTCTTCGTTGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGaa  <  2:62900/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              GGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTACCAGCAGAAGAAACCAGCTTGATTTTCTCACGAATACCTTTAGCCATGATTTATTTCCTCTAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGAA  >  NC_000913/3811190‑3811475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑