Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,193	G→A	L39F (CTT→TTT)	yidH ←	DUF202 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,193	0	G	A	100.0%	134.9 / NA	37	L39F (CTT→TTT)	yidH	DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

TCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAC  >  NC_000913/3856058‑3856340                                                                                                                                        |                                                                                                                                                    tCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCcgccgc                                                                                                                                      >  2:238539/1‑151 (MQ=255)       gCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 >  2:211813/1‑150 (MQ=255)       gCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 >  1:72931/1‑150 (MQ=255)        cATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 <  2:421725/149‑1 (MQ=255)        cATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAAt                                                                                                                               >  2:144656/1‑151 (MQ=255)         aTCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATc                                                                                                                              <  1:687625/151‑1 (MQ=255)            gCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCt                                                                                                                            >  1:277174/1‑150 (MQ=255)                   cATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAg                                                                                                                     <  1:248235/150‑1 (MQ=255)                            aCCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATcc                                                                                                            >  2:685524/1‑150 (MQ=255)                              cAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACg                                                                                                         >  2:555647/1‑151 (MQ=255)                              cAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACg                                                                                                         >  2:477645/1‑151 (MQ=255)                                       aaGCCATACATCGCAAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGaaaa                                                                                                 <  2:580232/149‑1 (MQ=255)                                         gCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGt                                                                                               >  2:275669/1‑150 (MQ=255)                                           cATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACg                                                                                            >  2:344762/1‑151 (MQ=255)                                            aTACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCAAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGc                                                                                           >  2:484809/1‑151 (MQ=255)                                              aCATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTc                                                                                         >  2:759992/1‑151 (MQ=255)                                                aTCGCTACCCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCAt                                                                                       <  2:400567/151‑1 (MQ=255)                                                             cgGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGaa                                                                          <  2:590934/151‑1 (MQ=255)                                                                   aCAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGt                                                                     >  1:710354/1‑150 (MQ=255)                                                                        cacaACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAg                                                               <  1:290906/151‑1 (MQ=255)                                                                             caacaacGCCAGGAGTTCGCGAATGACGGGCGTGGGGAAAGCTGGCGCAAGTTGGTCAAACCCAACCCCCGACGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGGACGCTGATTAGCCAGCGAGAAGCGGTAATCAGgcgc                                                           <  2:94697/150‑1 (MQ=255)                                                                             caacaacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCt                                                          >  1:415921/1‑151 (MQ=255)                                                                                   cGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCg                                                    <  1:760037/151‑1 (MQ=255)                                                                                       agcagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGc                                                >  2:235119/1‑151 (MQ=255)                                                                                            ttCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCggg                                             >  2:109168/1‑149 (MQ=255)                                                                                                   aTGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCa                                    <  1:685566/151‑1 (MQ=255)                                                                                                           cGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCt                            <  2:245912/151‑1 (MQ=255)                                                                                                             tGGCGCAATCTGGCCCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGGACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAATCGGTAATCAGGCGCTTCTCCGAGGCGGGGAATGTTCAatgatccta                           >  2:38503/1‑144 (MQ=255)                                                                                                                   aaTCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGc                     >  1:604360/1‑150 (MQ=255)                                                                                                                      cTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTTGATGTTTCTCCtttttgatt                 >  2:455805/1‑145 (MQ=255)                                                                                                                        ggCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAAt                  <  1:628141/148‑1 (MQ=255)                                                                                                                        ggCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTc               <  2:242428/151‑1 (MQ=255)                                                                                                                         gcgcAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCa              <  1:723628/151‑1 (MQ=255)                                                                                                                            cAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCCTTGTTGCTCCTTTGCAATTTCACt            >  2:758724/1‑150 (MQ=255)                                                                                                                               gTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACg        <  1:82870/151‑1 (MQ=255)                                                                                                                                tttaTCAAACCCAGCCGACGGCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTAATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTa           <  2:566387/144‑1 (MQ=255)                                                                                                                                     cAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAc  <  1:331727/151‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                    TCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAC  >  NC_000913/3856058‑3856340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑