Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,899,764	C→T	G272S (GGC→AGC)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,764	0	C	T	100.0%	166.2 / NA	41	G272S (GGC→AGC)	yieL	putative xylanase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/16);  total (25/16)

TCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGC  >  NC_000913/3899616‑3899911                                                                                                                                                     |                                                                                                                                                    tCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTAt                                                                                                                                                   >  1:478332/1‑151 (MQ=255)       aGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCAt                                                                                                                                             <  2:123041/151‑1 (MQ=255)           gggCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCttt                                                                                                                                         <  2:664305/151‑1 (MQ=255)            ggCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCttt                                                                                                                                         >  2:704246/1‑150 (MQ=255)               cgcgACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACgg                                                                                                                                     >  2:468887/1‑151 (MQ=255)                      ccTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTa                                                                                                                               <  2:154850/150‑1 (MQ=255)                             ccGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCtt                                                                                                                       <  2:373980/151‑1 (MQ=255)                                        gTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCg                                                                                                            <  1:661441/151‑1 (MQ=255)                                          cGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCggg                                                                                                          >  2:235303/1‑151 (MQ=255)                                          cGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCggg                                                                                                          >  2:228096/1‑151 (MQ=255)                                           gTGGTAACACCACTGAAGGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTTCCCGCCTTGTGAAAGCCCTGCCAGCGCTCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCggg                                                                                                          <  2:655371/150‑1 (MQ=255)                                              gTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGata                                                                                                       >  2:563307/1‑150 (MQ=255)                                                   accacTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCg                                                                                                 >  2:610547/1‑151 (MQ=255)                                                     cacTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGtt                                                                                               <  2:8206/151‑1 (MQ=255)                                                      acTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtc                                                                                              >  2:179024/1‑151 (MQ=255)                                                          aaTGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtcatca                                                                                          <  2:249628/151‑1 (MQ=255)                                                            tgtgGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGt                                                                                        >  2:471758/1‑151 (MQ=255)                                                             gtgGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGtt                                                                                       <  2:110099/151‑1 (MQ=255)                                                             gtgGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGt                                                                                        <  1:528211/150‑1 (MQ=255)                                                                     ccagccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCt                                                                               >  1:509201/1‑151 (MQ=255)                                                                            aaGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCtt                                                                        >  2:45663/1‑151 (MQ=255)                                                                                tttCCAGGTGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGc                                                                    >  2:201088/1‑151 (MQ=255)                                                                                    cAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTc                                                                >  2:517144/1‑151 (MQ=255)                                                                                          attcattcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCgg                                                           >  2:50996/1‑150 (MQ=255)                                                                                          attcattcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGa                                                          <  1:228105/151‑1 (MQ=255)                                                                                                 ccGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaaa                                                      >  1:725992/1‑148 (MQ=255)                                                                                                  cGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGAc                                                  >  2:259760/1‑151 (MQ=255)                                                                                                    gAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACtt                                                >  1:610975/1‑151 (MQ=255)                                                                                                    gAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACtt                                                >  2:183409/1‑151 (MQ=255)                                                                                                      aaCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCAAGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                               >  2:292969/1‑150 (MQ=255)                                                                                                          aGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTc                                          >  2:707750/1‑151 (MQ=255)                                                                                                           gcgcCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCt                                         <  1:300400/151‑1 (MQ=255)                                                                                                                 ggTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCt                                    <  2:395992/150‑1 (MQ=255)                                                                                                                 ggTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTg                                   <  1:58737/151‑1 (MQ=255)                                                                                                                      ccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGc                               >  1:582059/1‑150 (MQ=255)                                                                                                                       cGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCAc                             >  1:27567/1‑151 (MQ=255)                                                                                                                       cGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCAc                             <  2:509177/151‑1 (MQ=255)                                                                                                                                        gCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaata             >  2:490986/1‑150 (MQ=255)                                                                                                                                         ccAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaataat           <  1:517168/151‑1 (MQ=255)                                                                                                                                               cgcgGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCtt     >  2:632369/1‑151 (MQ=255)                                                                                                                                                  ggCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGc  >  2:722108/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGC  >  NC_000913/3899616‑3899911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑