Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,150,087	A→G	V60A (GTA→GCA)	eptC ←	LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,150,087	0	A	G	100.0%	148.9 / NA	40	V60A (GTA→GCA)	eptC	LPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

GGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAA  >  NC_000913/4149945‑4150233                                                                                                                                               |                                                                                                                                                   ggtTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGAt                                                                                                                                             <  1:745942/150‑1 (MQ=255)   tttCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCa                                                                                                                                           >  2:479734/1‑150 (MQ=255)     tCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAAcc                                                                                                                                        >  2:468825/1‑151 (MQ=255)      cGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACca                                                                                                                                       <  1:608612/151‑1 (MQ=255)          cATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACcac                                                                                                                                      <  2:170957/148‑1 (MQ=255)               caAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACt                                                                                                                              <  2:88556/151‑1 (MQ=255)               caAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACt                                                                                                                              >  1:29883/1‑151 (MQ=255)                  aCAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGaa                                                                                                                           <  1:247712/151‑1 (MQ=255)                     gAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATa                                                                                                                         >  1:145251/1‑150 (MQ=255)                      aaCGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAAc                                                                                                                       <  2:66237/151‑1 (MQ=255)                      aaCGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAAc                                                                                                                       <  2:149666/151‑1 (MQ=255)                        cGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAAc                                                                                                                       >  1:47408/1‑149 (MQ=255)                               cGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTcg                                                                                                              <  1:63295/151‑1 (MQ=255)                                gagaACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTcgc                                                                                                             >  2:289064/1‑151 (MQ=255)                                 agaACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTcgcg                                                                                                            <  1:82825/151‑1 (MQ=255)                                        cTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCg                                                                                                     >  1:231884/1‑151 (MQ=255)                                                aGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGcc                                                                                             >  1:284717/1‑151 (MQ=255)                                                       gtagtaGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATaa                                                                                      <  2:432501/151‑1 (MQ=255)                                                         agtagCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAAcc                                                                                    >  1:66962/1‑151 (MQ=255)                                                            agCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACt                                                                                 >  1:686150/1‑151 (MQ=255)                                                                   cgccgcCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTaa                                                                          <  2:437288/151‑1 (MQ=255)                                                                            agagGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGcc                                                                 <  1:457681/151‑1 (MQ=255)                                                                             gagGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCt                                                                <  2:350265/151‑1 (MQ=255)                                                                                    cccATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCag                                                         <  1:463848/151‑1 (MQ=255)                                                                                         aGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTa                                                     <  1:737861/150‑1 (MQ=255)                                                                                          gCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTaga                                                   <  1:292907/151‑1 (MQ=255)                                                                                              cacGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGaaa                                               <  1:104552/151‑1 (MQ=255)                                                                                                cGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGaaaa                                              >  1:275834/1‑150 (MQ=255)                                                                                                    gATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCa                                          >  1:15946/1‑150 (MQ=255)                                                                                                     aTTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCaaa                                        <  1:220631/151‑1 (MQ=255)                                                                                                        aCTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGt                                     <  1:382261/151‑1 (MQ=255)                                                                                                             ggCAATAATTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGc                                <  2:272493/151‑1 (MQ=255)                                                                                                                aataatTTTAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGt                             <  2:252701/151‑1 (MQ=255)                                                                                                                        tAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAAcc                     >  2:501064/1‑151 (MQ=255)                                                                                                                        tAATCCGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAAGAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAAc                      <  2:249714/150‑1 (MQ=255)                                                                                                                            ccGCTTCGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAgg                 <  2:237646/151‑1 (MQ=255)                                                                                                                                  cGGAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCttt            <  2:54682/150‑1 (MQ=255)                                                                                                                                   ggAAAGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTcc          >  2:368830/1‑151 (MQ=255)                                                                                                                                      aaGAGGAATGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGc       >  1:751765/1‑151 (MQ=255)                                                                                                                                            aaTGCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTaaaa  >  2:223278/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                   GGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAA  >  NC_000913/4149945‑4150233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑