Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,334,684	C→T	A303T (GCA→ACA)	eptA ←	lipid A phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,334,684	0	C	T	100.0%	214.2 / NA	55	A303T (GCA→ACA)	eptA	lipid A phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/31);  total (24/31)

ACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGCCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCGGGTAGCCGTT  >  NC_000913/4334543‑4334831                                                                                                                                              |                                                                                                                                                    aCTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCtcttc                                                                                                                                            <  2:306837/151‑1 (MQ=255)     aTCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTg                                                                                                                                        >  2:433147/1‑151 (MQ=255)          gTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTg                                                                                                                                   <  2:418624/151‑1 (MQ=255)              aTTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTc                                                                                                                                >  1:640786/1‑150 (MQ=255)                 cAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCGCCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCAcg                                                                                                                             <  2:176283/150‑1 (MQ=255)                  aGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCAcgcg                                                                                                                           >  1:469977/1‑151 (MQ=255)                   gcgcGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCgg                                                                                                                          <  1:108195/151‑1 (MQ=255)                    cgcgGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGc                                                                                                                         >  2:87892/1‑151 (MQ=255)                     gcgGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGc                                                                                                                         >  1:236263/1‑150 (MQ=255)                     gcgGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGc                                                                                                                         >  1:37074/1‑150 (MQ=255)                             gTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCgag                                                                                                                <  2:512451/151‑1 (MQ=255)                             gTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCg                                                                                                                  >  1:680881/1‑149 (MQ=255)                                   gTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATg                                                                                                          <  1:192673/151‑1 (MQ=255)                                    tGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGc                                                                                                         >  2:727838/1‑151 (MQ=255)                                        gCACGCGGTCGCAGGCACCTTTCAAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCATGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACAt                                                                                                           <  2:302511/145‑1 (MQ=255)                                                  gCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACt                                                                                           <  1:314598/151‑1 (MQ=255)                                                    aGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACtgc                                                                                         >  1:506597/1‑151 (MQ=255)                                                       cACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACtgccgt                                                                                      <  2:390720/151‑1 (MQ=255)                                                         ccTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACtgccgt                                                                                      <  1:635350/149‑1 (MQ=255)                                                              aCAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTtgccgtg                                                                               <  1:451325/151‑1 (MQ=255)                                                                 gccgccATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTtgccgtgccg                                                                            <  2:466158/151‑1 (MQ=255)                                                                 gccgccATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTtgccgtgccg                                                                            <  2:327701/151‑1 (MQ=255)                                                                 gccgccATCCTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTtgccgtg                                                                               <  2:133569/148‑1 (MQ=255)                                                                    gccATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCa                                                                          >  2:744509/1‑150 (MQ=255)                                                                     ccATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAg                                                                        <  1:248480/151‑1 (MQ=255)                                                                       aTCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGAt                                                                      <  1:575056/151‑1 (MQ=255)                                                                        tCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATg                                                                     >  2:230189/1‑151 (MQ=255)                                                                         cGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGc                                                                    <  1:232078/151‑1 (MQ=255)                                                                           ttGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCgg                                                                  >  2:745089/1‑151 (MQ=255)                                                                             gTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGt                                                                 <  1:138943/150‑1 (MQ=255)                                                                                aTTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTa                                                             <  1:142669/151‑1 (MQ=255)                                                                                       aGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCGGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAAt                                                      <  2:332370/151‑1 (MQ=255)                                                                                         cACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAg                                                    <  1:500037/151‑1 (MQ=255)                                                                                           cGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGAc                                                  >  1:269286/1‑151 (MQ=255)                                                                                               gATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCAc                                               <  1:464731/150‑1 (MQ=255)                                                                                                      gctcgctGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCtt                                       >  1:286158/1‑151 (MQ=255)                                                                                                            tGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGcc                                  >  2:28489/1‑150 (MQ=255)                                                                                                            tGAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGcc                                  >  2:351074/1‑150 (MQ=255)                                                                                                             gAATGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAg                                <  1:195305/151‑1 (MQ=255)                                                                                                              aaTGATATCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGc                               <  2:37073/151‑1 (MQ=255)                                                                                                                  atatCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCcgcg                           <  2:211856/151‑1 (MQ=255)                                                                                                                  atatCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCcgcg                           <  1:57846/151‑1 (MQ=255)                                                                                                                    atCCAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCggg                         >  2:387644/1‑151 (MQ=255)                                                                                                                       cAGCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTa                      <  2:435338/151‑1 (MQ=255)                                                                                                                         gCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGt                    >  1:487190/1‑151 (MQ=255)                                                                                                                         gCACGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGt                    >  2:52144/1‑151 (MQ=255)                                                                                                                           aCGCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGttt                  >  2:548118/1‑151 (MQ=255)                                                                                                                             gCCTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTc                 >  1:516620/1‑150 (MQ=255)                                                                                                                              cctgcctgGTGCTGTGTCAGCTCGTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTGAGTTTCAc               <  2:706616/151‑1 (MQ=255)                                                                                                                              ccTTCCTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCAc               >  1:328818/1‑151 (MQ=255)                                                                                                                                  ccTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCgg           <  1:656178/151‑1 (MQ=255)                                                                                                                                   cTGGTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCggg          >  2:715555/1‑151 (MQ=255)                                                                                                                                     ggTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCGGGTa        <  2:435261/151‑1 (MQ=255)                                                                                                                                      gTGCTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGTCGAAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCTGCCGCGGGTTAGTTTCACGCGGGTAg       <  2:660547/151‑1 (MQ=255)                                                                                                                                         cTGTGTCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCGGGTAGcc     >  2:697887/1‑150 (MQ=255)                                                                                                                                           gtgtCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCGGGTAGCCGtt  >  2:337371/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    ACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACGCGGTCGCAGGCACCTTTACAGCCGCCATCGTTGTCATTCCACAGCACGTTGATGCCCGCTCGCTGAATGATATCCAGCACGCCTTCCTGGTGCTGTGCCAGCTCTTCTTTGTAGTGCTCACGCGGCATATCCGAGAACATGCACGGTACTGAAACTGCCGTTGCCGTGCCGCAAGATGCGGTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTCACGCGGGTAGCCGTT  >  NC_000913/4334543‑4334831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑