Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,878	T→C	T36A (ACG→GCG)	ytfE ←	iron‑sulfur cluster repair protein RIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,878	0	T	C	100.0%	97.7 / NA	31	T36A (ACG→GCG)	ytfE	iron‑sulfur cluster repair protein RIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/27);  total (4/27)

CGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG  >  NC_000913/4431743‑4432015                                                                                                                                        |                                                                                                                                          cGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCcac                                                                                                                             <  1:762708/150‑1 (MQ=255)     gatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCAcagcag                                                                                                                        <  1:663686/151‑1 (MQ=255)                               gggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACag                                                                                              <  1:185435/151‑1 (MQ=255)                                  cgcTCCGCCGGTCTTTCTCAATCGGTTGTTCAGCGGGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCATTTCTTTACGTCCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACaga                                                                                             <  2:6829/149‑1 (MQ=255)                                  cgcTACGCCAGTCTTTCTCAATCGGTTGTGCAGCGAGCTTTGCCAGTTCAGCTTCAAGGACCTCAACATCCAGTTCTTTACCTGCCGCCGCGCGCCCCAGCGCATGCTTACCGCCACAGCAGTAATCCATATCATATTTCCGAAACagag                                                                                            <  2:374706/150‑1 (MQ=255)                                     tACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTg                                                                                         >  1:243007/1‑150 (MQ=255)                                      aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCGGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGa                                                                                        <  2:479037/150‑1 (MQ=255)                                          cAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAgcgc                                                                                   <  1:190881/151‑1 (MQ=255)                                                ttCTCAATCGTTTGTTCAGCGAGCTTTGCAAGTTCAGCTTCAATGTCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCGAGCGCCTGCTTGCCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAg                                                                                <  2:166145/148‑1 (MQ=255)                                                 tctcAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGaa                                                                             <  2:318031/150‑1 (MQ=255)                                                   tcAATCGGTTGTTCAGCAAGCTTTGCCAGTTCAGCTTCAATGACCTAAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAAt                                                                            <  1:540352/149‑1 (MQ=255)                                                   tcAATCGGTTGTTCAGCAAGCTTTGCCAGTTCAGCTTCAATAACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGaa                                                                             >  2:540324/1‑148 (MQ=255)                                                    cAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATag                                                                          <  1:740167/150‑1 (MQ=255)                                                        cGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAgc                                                                     <  1:143841/151‑1 (MQ=255)                                                         ggTTTTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATagaga                                                                       <  1:358826/148‑1 (MQ=255)                                                            tgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGcc                                                                  <  1:104145/150‑1 (MQ=255)                                                             gttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCa                                                                 <  2:401829/150‑1 (MQ=255)                                                                          tGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAgg                                                    <  1:334254/150‑1 (MQ=255)                                                                                                 cAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTg                             >  2:68539/1‑150 (MQ=255)                                                                                                      tCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                        <  2:413887/150‑1 (MQ=255)                                                                                                      tCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                       >  2:240427/1‑151 (MQ=255)                                                                                                       ccAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                       <  1:434356/150‑1 (MQ=255)                                                                                                           ttCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAtt                   <  1:129874/150‑1 (MQ=255)                                                                                                           ttCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAt                    <  1:385320/149‑1 (MQ=255)                                                                                                            tCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                  <  2:501871/150‑1 (MQ=255)                                                                                                            tCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                  <  2:199391/150‑1 (MQ=255)                                                                                                                 aCGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaat             <  2:672607/150‑1 (MQ=255)                                                                                                                 aCGGGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaata            <  2:400379/151‑1 (MQ=255)                                                                                                                    tGCCGCCGCGCGCGCCAGCGCATGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataag          <  2:409976/150‑1 (MQ=255)                                                                                                                     gccgccGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagat        <  2:250384/151‑1 (MQ=255)                                                                                                                           gcgcgcgcCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATg  <  2:398507/151‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          CGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG  >  NC_000913/4431743‑4432015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑