Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,550,829 C→T L156L (CTG→CTA gntP ← fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,550,8290CT100.0% 223.4 / NA 58L156L (CTG→CTAgntPfructuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/31);  total (27/31)

GCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAACAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCG  >  NC_000913/4550682‑4550976
                                                                                                                                                   |                                                                                                                                                   
gcgcTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGt                                                                                                                                                  >  1:26645/1‑151 (MQ=255)
  gcTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTg                                                                                                                                                 >  1:540601/1‑150 (MQ=255)
            gCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGt                                                                                                                                       <  2:194387/150‑1 (MQ=255)
            gCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGt                                                                                                                                       <  1:36557/150‑1 (MQ=255)
               gAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTg                                                                                                                                   <  2:18683/151‑1 (MQ=255)
                aGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGGCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTgg                                                                                                                                  >  2:293094/1‑151 (MQ=255)
                     cTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAgct                                                                                                                             >  1:520143/1‑151 (MQ=255)
                         ggCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAgctgct                                                                                                                          >  1:677827/1‑150 (MQ=255)
                           cAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACt                                                                                                                       >  1:482234/1‑151 (MQ=255)
                            aGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTg                                                                                                                      >  1:502623/1‑151 (MQ=255)
                            aGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTg                                                                                                                      <  1:454688/151‑1 (MQ=255)
                             ggATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTCCGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGc                                                                                                                     <  2:315892/151‑1 (MQ=255)
                               aTCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCg                                                                                                                   <  1:670246/151‑1 (MQ=255)
                                  aGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGa                                                                                                                <  1:245376/151‑1 (MQ=255)
                                      cTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGc                                                                                                            >  1:285458/1‑151 (MQ=255)
                                        gcgcAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCa                                                                                                          <  1:77590/151‑1 (MQ=255)
                                          gcAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGt                                                                                                        >  1:517789/1‑151 (MQ=255)
                                            aGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGttt                                                                                                      <  1:317773/151‑1 (MQ=255)
                                                       gggATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGa                                                                                            <  2:629809/150‑1 (MQ=255)
                                                       gggATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGa                                                                                            >  2:428118/1‑150 (MQ=255)
                                                       gggATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGa                                                                                            <  1:186722/150‑1 (MQ=255)
                                                         gATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAAtt                                                                                         >  2:365584/1‑151 (MQ=255)
                                                              tCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGc                                                                                    >  1:18652/1‑151 (MQ=255)
                                                                  cAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTcg                                                                                <  1:18381/151‑1 (MQ=255)
                                                                        gCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCggcggc                                                                           >  1:645509/1‑150 (MQ=255)
                                                                        gCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCggcggc                                                                           >  2:206313/1‑150 (MQ=255)
                                                                           aTAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCggcggcca                                                                         >  1:383719/1‑147 (MQ=255)
                                                                              gATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTACAGGAACGGAATTTTAGCTTCGGCGGcaataac                                                                     <  2:731168/150‑1 (MQ=255)
                                                                               atatAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGcaataacaa                                                                   <  1:71759/151‑1 (MQ=255)
                                                                                       cATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAgc                                                           <  1:710702/151‑1 (MQ=255)
                                                                                        aTCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAgcg                                                          <  1:219932/151‑1 (MQ=255)
                                                                                        aTCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAgc                                                           >  2:534865/1‑150 (MQ=255)
                                                                                          ccccATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAgcggc                                                        >  1:662814/1‑151 (MQ=255)
                                                                                            ccATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGgcgc                                                      <  2:124546/151‑1 (MQ=255)
                                                                                            ccATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAACGgcgc                                                      >  2:406245/1‑151 (MQ=255)
                                                                                                    gcgcCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGa                                              <  1:563891/151‑1 (MQ=255)
                                                                                                      gtcATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGCATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGAt                                             >  1:83267/3‑150 (MQ=255)
                                                                                                       ccATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATaa                                           <  1:656856/151‑1 (MQ=255)
                                                                                                       ccATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATaa                                           <  1:430863/151‑1 (MQ=255)
                                                                                                        cATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGATACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATaa                                           >  2:624164/1‑150 (MQ=255)
                                                                                                                ttCACCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACtt                                  >  1:413645/1‑151 (MQ=255)
                                                                                                                   aCCAGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCAt                               <  2:371840/151‑1 (MQ=255)
                                                                                                                    ccAGCGCCACCGGACACGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCCGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATa                              <  1:168577/151‑1 (MQ=255)
                                                                                                                      aGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCAt                               >  1:728144/1‑148 (MQ=255)
                                                                                                                      aGCGCCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCAt                               >  1:563085/1‑148 (MQ=255)
                                                                                                                        cgcCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAAc                          <  2:20003/151‑1 (MQ=255)
                                                                                                                         gcCACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACa                         <  1:678277/151‑1 (MQ=255)
                                                                                                                           cACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATc                       >  2:304483/1‑151 (MQ=255)
                                                                                                                           cACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATc                       <  2:520119/151‑1 (MQ=255)
                                                                                                                           cACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTACATGATAAAGGCCACTTCATAGAACATCg                      <  2:18652/151‑1 (MQ=255)
                                                                                                                           cACCGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGAGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATc                       >  2:143379/1‑151 (MQ=255)
                                                                                                                             ccGGACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGc                     <  2:54720/151‑1 (MQ=255)
                                                                                                                               ggACCCGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCa                   <  2:451217/151‑1 (MQ=255)
                                                                                                                                   ccGGCTGCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGAcc               <  1:16069/151‑1 (MQ=255)
                                                                                                                                         gCGGTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAAt         <  2:756236/151‑1 (MQ=255)
                                                                                                                                            gTGGGAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATc        >  1:726988/1‑149 (MQ=255)
                                                                                                                                                gAATAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCg  <  1:201298/151‑1 (MQ=255)
                                                                                                                                                gAATAGTGAATGTGCGGGAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGACCATCGCCAGACCAAAAATCAAGcc   >  2:706402/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGCTCAAGATTGCCGAGGAACTTCGGCAGGATCAGACCTGCGCAGATTACACTTGGGATCGTCACCAGTACGCCATAGATATAAACCATCCCCATATCCGCGCCATAAGCATTCACCAGCGCCACCGGACCCGGCTGCGGTGGGAACAGTGAATGTGCGGTAGTGGCAGCTGCTACTGCCGGGATCGCCAGTTTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCG  >  NC_000913/4550682‑4550976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: