Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,916	A→G	T53A (ACT→GCT)	trpR →	transcriptional repressor, tryptophan‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,916	0	A	G	100.0%	270.1 / NA	76	T53A (ACT→GCT)	trpR	transcriptional repressor, tryptophan‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/45);  total (31/45)

ACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGG  >  NC_000913/4632768‑4633060                                                                                                                                                     |                                                                                                                                                 acaaTCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCt                                                                                                                                                <  2:654630/151‑1 (MQ=255)  caaTCACCCTATTCAGCAGCGATGGCAGAACAGCTTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAACCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTc                                                                                                                                               <  2:271045/151‑1 (MQ=255)      cACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTcgcg                                                                                                                                            <  1:509317/150‑1 (MQ=255)         ccTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCgcgtgcg                                                                                                                                        >  1:131650/1‑151 (MQ=255)             ttCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTAtt                                                                                                                                    >  1:494864/1‑151 (MQ=255)               cagcagCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGt                                                                                                                                  >  1:44868/1‑151 (MQ=255)                  cagcGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGa                                                                                                                               <  2:646244/151‑1 (MQ=255)                    gcGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAag                                                                                                                             <  2:331318/151‑1 (MQ=255)                     cGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGa                                                                                                                               >  1:446518/1‑148 (MQ=255)                       aTGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGc                                                                                                                          <  2:10783/151‑1 (MQ=255)                          gCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGt                                                                                                                       >  2:634850/1‑151 (MQ=255)                            aGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTg                                                                                                                     <  2:220962/151‑1 (MQ=255)                                cAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGcgcg                                                                                                                 <  1:152727/151‑1 (MQ=255)                                 aGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCgcgg                                                                                                                <  2:525728/151‑1 (MQ=255)                                      cACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGaa                                                                                                            <  2:147540/150‑1 (MQ=255)                                       aCCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAAt                                                                                                          <  1:187017/151‑1 (MQ=255)                                        ccAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATg                                                                                                         >  1:70179/1‑151 (MQ=255)                                          aGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAg                                                                                                       <  2:588727/151‑1 (MQ=255)                                          aGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAg                                                                                                       <  2:607551/151‑1 (MQ=255)                                            gAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGcc                                                                                                     >  2:108692/1‑151 (MQ=255)                                             aGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCa                                                                                                    <  1:254448/151‑1 (MQ=255)                                                ggTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGc                                                                                                  <  2:138055/150‑1 (MQ=255)                                                   tACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTg                                                                                               >  1:590690/1‑150 (MQ=255)                                                      gTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGtt                                                                                           >  2:296273/1‑151 (MQ=255)                                                          tGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTaaa                                                                                        <  2:273787/150‑1 (MQ=255)                                                          tGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTaaa                                                                                        <  2:468504/150‑1 (MQ=255)                                                           gTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTaaaaa                                                                                      <  1:378447/151‑1 (MQ=255)                                                           gTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTaaaa                                                                                       <  2:177998/150‑1 (MQ=255)                                                           gTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTaaa                                                                                        <  2:322378/149‑1 (MQ=255)                                                               aCCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGa                                                                                  <  2:49747/151‑1 (MQ=255)                                                                ccTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGaa                                                                                 <  1:419898/151‑1 (MQ=255)                                                                   gCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAAc                                                                                <  1:104740/149‑1 (MQ=255)                                                                   gCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAAc                                                                                <  1:112171/149‑1 (MQ=255)                                                                      tAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCgg                                                                            <  1:103938/150‑1 (MQ=255)                                                                       aaGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGgcg                                                                          <  1:609450/151‑1 (MQ=255)                                                                         gAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCa                                                                        <  2:553005/151‑1 (MQ=255)                                                                         gAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCa                                                                        <  2:71660/151‑1 (MQ=255)                                                                           aTGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAgg                                                                      >  1:422873/1‑151 (MQ=255)                                                                            tGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGc                                                                     >  1:756246/1‑151 (MQ=255)                                                                               cTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATc                                                                  >  2:389132/1‑151 (MQ=255)                                                                                  ccAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATcgc                                                                >  2:343556/1‑150 (MQ=255)                                                                                   cAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGcga                                                              <  1:617205/151‑1 (MQ=255)                                                                                      aaCGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGcgacga                                                           <  1:478634/151‑1 (MQ=255)                                                                                         gATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGAtt                                                         >  1:295797/1‑150 (MQ=255)                                                                                            ctcCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGAtt                                                         >  2:484118/1‑147 (MQ=255)                                                                                              ccATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTAcg                                                      <  1:48591/148‑1 (MQ=255)                                                                                               cATTTACCGTTGTTAAACCTGATGCTGCCGCCAGATGAGCCCGAAGCGTTGGGGGCTCGCGTGCGTATTGCCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGGGGGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTaggatcg                                                  >  2:396659/1‑145 (MQ=255)                                                                                               cATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTa                                                        <  1:396680/145‑1 (MQ=255)                                                                                                   tACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGAt                                               <  1:434977/150‑1 (MQ=255)                                                                                                     ccGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCt                                             >  2:674097/1‑150 (MQ=255)                                                                                                     ccGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTa                                            <  2:747798/151‑1 (MQ=255)                                                                                                         tgttAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAAc                                          >  2:290717/1‑149 (MQ=255)                                                                                                           ttAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGcc                                      >  2:461440/1‑151 (MQ=255)                                                                                                            tAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGcc                                      <  1:346454/150‑1 (MQ=255)                                                                                                             aaaCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCt                                     <  2:674038/150‑1 (MQ=255)                                                                                                             aaaCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTg                                    <  2:413065/151‑1 (MQ=255)                                                                                                             aaaCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTg                                    >  2:405259/1‑151 (MQ=255)                                                                                                               aCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGaa                                  <  1:325766/151‑1 (MQ=255)                                                                                                               aCCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGa                                   >  1:365748/1‑150 (MQ=255)                                                                                                                        tGACGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGcc                         >  1:287870/1‑151 (MQ=255)                                                                                                                          aCGCCAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCg                       >  1:745332/1‑151 (MQ=255)                                                                                                                             ccAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCg                    <  2:304985/151‑1 (MQ=255)                                                                                                                              cAGATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCg                    >  2:334023/1‑150 (MQ=255)                                                                                                                                gATGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGc                 <  1:754115/151‑1 (MQ=255)                                                                                                                                 aTGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCt                >  1:118696/1‑151 (MQ=255)                                                                                                                                  tGAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTg               >  1:222738/1‑151 (MQ=255)                                                                                                                                   gAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTgc              <  1:357422/151‑1 (MQ=255)                                                                                                                                   gAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTgc              <  1:684022/151‑1 (MQ=255)                                                                                                                                   gAGCGCGAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTg               <  2:5632/150‑1 (MQ=255)                                                                                                                                       gcgAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGcc           <  2:487917/150‑1 (MQ=255)                                                                                                                                         gAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAg         >  2:487841/1‑150 (MQ=255)                                                                                                                                         gAAGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGt        >  2:710063/1‑151 (MQ=255)                                                                                                                                           aGCGTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTgg      >  2:729984/1‑151 (MQ=255)                                                                                                                                              gTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTg   <  1:143813/151‑1 (MQ=255)                                                                                                                                              gTTGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTg   >  1:734028/1‑151 (MQ=255)                                                                                                                                               ttGGGGGCTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTgg  >  2:658503/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 ACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGG  >  NC_000913/4632768‑4633060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑