Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,316	C→G	A101P (GCC→CCC)	yaiS ←	putative PIG‑L family deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,316	0	C	G	100.0%	84.5 / NA	24	A101P (GCC→CCC)	yaiS	putative PIG‑L family deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (6/18);  total (6/18)

AGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTG  >  NC_000913/384171‑384443                                                                                                                                                  |                                                                                                                                agaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGaaa                                                                                                                            <  2:668316/151‑1 (MQ=255)   aaTTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatc                                                                                                                          <  2:264365/151‑1 (MQ=255)        gCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatcatat                                                                                                                      <  1:473552/150‑1 (MQ=255)           gAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCAtt                                                                                                                  <  1:10772/151‑1 (MQ=255)             aTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGa                                                                                                                <  2:284179/151‑1 (MQ=255)                       aggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATg                                                                                                      <  2:123827/151‑1 (MQ=255)                            aCCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAgcgc                                                                                                 <  2:337969/151‑1 (MQ=255)                                     gCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTc                                                                                          >  1:435576/1‑149 (MQ=255)                                     gCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTc                                                                                          >  1:474154/1‑149 (MQ=255)                                     gCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAg                                                                                        <  1:633662/151‑1 (MQ=255)                                        tGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGc                                                                                       <  2:398189/149‑1 (MQ=255)                                         gATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCaa                                                                                     <  1:677701/150‑1 (MQ=255)                                          aTAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCaaaa                                                                                   <  1:117083/151‑1 (MQ=255)                                             aaCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTa                                                                                <  2:235122/151‑1 (MQ=255)                                              aCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCaaa                                                                                    >  1:485296/1‑146 (MQ=255)                                              aCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCaaa                                                                                    <  2:485273/146‑1 (MQ=255)                                                                       cGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTa                                                       >  1:6889/1‑150 (MQ=255)                                                                            tcatGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCCGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAat                                                     >  1:387284/1‑147 (MQ=255)                                                                                               gCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttc                              <  2:216043/151‑1 (MQ=255)                                                                                                   gATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttcttc                           <  2:186775/150‑1 (MQ=255)                                                                                                                gAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGatt              >  1:346624/1‑150 (MQ=255)                                                                                                                      aTTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCAt       <  1:459407/151‑1 (MQ=255)                                                                                                                         tGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCt     <  1:186249/150‑1 (MQ=255)                                                                                                                           aTTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCtgtg  <  2:747068/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                AGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTG  >  NC_000913/384171‑384443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑