Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	493,696	G→A	L535L (CTG→CTA)	dnaX →	DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	493,696	0	G	A	100.0%	201.4 / NA	52	L535L (CTG→CTA)	dnaX	DNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

AAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTCAACG  >  NC_000913/493551‑493840                                                                                                                                                  |                                                                                                                                                 aaaaaGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTc                                                                                                                                               <  2:622047/149‑1 (MQ=255)       aagTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTGGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTTCCAAAACTAGTCGAACAGGt                                                                                                                                       <  2:419827/151‑1 (MQ=255)       aagTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCACGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGt                                                                                                                                       <  1:420902/151‑1 (MQ=255)              cGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGtt                                                                                                                                 >  2:754915/1‑150 (MQ=255)                  aCGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAAt                                                                                                                             >  1:514293/1‑150 (MQ=255)                   cGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATg                                                                                                                            <  2:719247/150‑1 (MQ=255)                    gCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACTAAAACTAGTCGAACAGGTGGCGTTAAATGcc                                                                                                                          >  2:320126/1‑151 (MQ=255)                     ccGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCt                                                                                                                         >  2:704371/1‑151 (MQ=255)                     ccGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCt                                                                                                                         >  2:543205/1‑151 (MQ=255)                             gcTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAag                                                                                                                   >  1:490943/1‑149 (MQ=255)                                   aaaaGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGc                                                                                                           >  1:38798/1‑151 (MQ=255)                                    aaaGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCg                                                                                                          >  2:587229/1‑151 (MQ=255)                                         gcTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAAc                                                                                                     >  2:680817/1‑151 (MQ=255)                                            ggAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACg                                                                                                    <  2:36601/149‑1 (MQ=255)                                            ggAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGc                                                                                                   <  1:25734/150‑1 (MQ=255)                                              aaCATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCa                                                                                                  <  2:683757/149‑1 (MQ=255)                                              aaCATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCa                                                                                                  >  1:683799/1‑149 (MQ=255)                                                cATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTAt                                                                                              <  1:651689/151‑1 (MQ=255)                                                 aTGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATg                                                                                             >  1:535632/1‑151 (MQ=255)                                                   gAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGt                                                                                            <  1:587265/150‑1 (MQ=255)                                                          cGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCAttt                                                                                    >  1:650261/1‑151 (MQ=255)                                                           gCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTg                                                                                   >  2:332446/1‑151 (MQ=255)                                                                      cggcgAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCtctc                                                                         >  1:286549/1‑150 (MQ=255)                                                                       ggcgAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCtctc                                                                         >  2:236374/1‑149 (MQ=255)                                                                        gcgAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGc                                                                      >  2:413474/1‑151 (MQ=255)                                                                                aGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTg                                                              <  2:409822/151‑1 (MQ=255)                                                                                aGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTg                                                              <  1:453985/151‑1 (MQ=255)                                                                                 gCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGa                                                             <  2:578398/151‑1 (MQ=255)                                                                                     cAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGaaca                                                          >  2:248847/1‑150 (MQ=255)                                                                                             aTTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTg                                                 >  1:252722/1‑151 (MQ=255)                                                                                             aTTGAGCGCGACCCGTGGGCGGCACAGGTGAGCAAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTg                                                 >  1:98977/1‑151 (MQ=255)                                                                                                gAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGcac                                              >  2:232580/1‑151 (MQ=255)                                                                                                    gcgACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGc                                           >  1:11191/1‑150 (MQ=255)                                                                                                    gcgACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCa                                          <  1:611616/151‑1 (MQ=255)                                                                                                      gACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCaa                                         >  1:166850/1‑150 (MQ=255)                                                                                                         ccGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAAc                                     >  1:365870/1‑151 (MQ=255)                                                                                                           gTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACt                                    >  2:715013/1‑150 (MQ=255)                                                                                                           gTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTg                                   >  1:681015/1‑151 (MQ=255)                                                                                                              ggcggcACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCt                                <  2:286537/151‑1 (MQ=255)                                                                                                                  gcACAGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAg                            <  2:264934/151‑1 (MQ=255)                                                                                                                      aGGTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGtt                        >  1:374784/1‑151 (MQ=255)                                                                                                                       ggTGAGCCAACTTTCGCTGCCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTg                       <  2:299011/151‑1 (MQ=255)                                                                                                                       ggTGAGCCAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTg                       <  1:346586/151‑1 (MQ=255)                                                                                                                             ccAACTTTCGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATg                 <  2:30372/151‑1 (MQ=255)                                                                                                                                     cGCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAgg         >  1:490386/1‑151 (MQ=255)                                                                                                                                      gCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGt        >  2:611313/1‑151 (MQ=255)                                                                                                                                      gCTACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGt        <  2:483112/151‑1 (MQ=255)                                                                                                                                        tACCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTc      >  1:245951/1‑151 (MQ=255)                                                                                                                                         aCCAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGtt       <  2:83861/149‑1 (MQ=255)                                                                                                                                          ccAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTCaa    <  2:744724/151‑1 (MQ=255)                                                                                                                                           cAAAACTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTCAAc   <  1:599383/151‑1 (MQ=255)                                                                                                                                            aaaaCTAGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTCAACg  <  1:133071/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 AAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCATGTTAAAAGGTTCAACG  >  NC_000913/493551‑493840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑