Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	496,700	T→C	R527R (CGT→CGC)	htpG →	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	496,700	0	T	C	100.0%	248.7 / NA	65	R527R (CGT→CGC)	htpG	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/39);  total (26/39)

AACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGTCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTG  >  NC_000913/496552‑496847                                                                                                                                                     |                                                                                                                                                    aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCt                                                                                                                                                   <  2:648781/151‑1 (MQ=255)   ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTg                                                                                                                                                  <  2:104767/150‑1 (MQ=255)           gTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGt                                                                                                                                         >  2:335011/1‑151 (MQ=255)           gTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGt                                                                                                                                         >  1:671604/1‑151 (MQ=255)              ggTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTg                                                                                                                                      <  2:411821/151‑1 (MQ=255)               gtgtCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGATAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGa                                                                                                                                     <  2:253935/151‑1 (MQ=255)                 gtCTAAAGGTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGAc                                                                                                                                    <  2:531585/150‑1 (MQ=255)                     aaaGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCg                                                                                                                                  <  2:53434/148‑1 (MQ=255)                     aaaGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGAt                                                                                                                                >  2:593731/1‑150 (MQ=255)                      aaGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATAc                                                                                                                              <  1:115712/151‑1 (MQ=255)                      aaGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATAc                                                                                                                              >  1:23472/1‑151 (MQ=255)                            aCGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGc                                                                                                                        <  1:74371/151‑1 (MQ=255)                             cGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCg                                                                                                                       >  1:392938/1‑151 (MQ=255)                             cGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCg                                                                                                                       <  1:393342/151‑1 (MQ=255)                                    cTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGttt                                                                                                                >  1:646804/1‑151 (MQ=255)                                      tGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGttt                                                                                                                <  2:683200/149‑1 (MQ=255)                                       gAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTcga                                                                                                             <  1:640269/151‑1 (MQ=255)                                       gAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTcg                                                                                                              >  2:473381/1‑150 (MQ=255)                                       gAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTcg                                                                                                              <  1:260142/150‑1 (MQ=255)                                        aaaaaCTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTcgac                                                                                                            >  1:415410/1‑151 (MQ=255)                                        aaaaaCTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCCTGAAAGATGTCCGCCTGCCTCACCGTCTGACCGATACGCCAGCGATCGTTTcgac                                                                                                            <  2:350113/151‑1 (MQ=255)                                               ggCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGAcgcg                                                                                                     <  2:358048/151‑1 (MQ=255)                                               ggCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGAcg                                                                                                       <  1:102742/149‑1 (MQ=255)                                                  tGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGAc                                                                                                  >  2:384582/1‑151 (MQ=255)                                                   gACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACg                                                                                                 >  2:187838/1‑151 (MQ=255)                                                       aaGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAAt                                                                                             <  1:370342/151‑1 (MQ=255)                                                            gatgaGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGATGAAATGAGCa                                                                                        <  2:386276/151‑1 (MQ=255)                                                            gatgaGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGc                                                                                         <  1:568010/150‑1 (MQ=255)                                                            gatgaGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGc                                                                                         <  2:58470/150‑1 (MQ=255)                                                              tgaGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACt                                                                                      >  1:84677/1‑151 (MQ=255)                                                                agagCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCa                                                                                    <  1:224224/151‑1 (MQ=255)                                                                 gagCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAg                                                                                   <  2:46740/151‑1 (MQ=255)                                                                  agCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTc                                                                                     >  2:453130/1‑148 (MQ=255)                                                                  agCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGa                                                                                  <  2:615932/151‑1 (MQ=255)                                                                   gcgcGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAg                                                                                   <  1:431873/149‑1 (MQ=255)                                                                       gAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCg                                                                             <  1:761322/151‑1 (MQ=255)                                                                               cGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGtt                                                                     <  1:183835/151‑1 (MQ=255)                                                                                      aGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTgcg                                                              <  1:219578/151‑1 (MQ=255)                                                                                       gCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTgcgg                                                             <  2:175670/151‑1 (MQ=255)                                                                                        cACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTgcggc                                                            <  1:386125/151‑1 (MQ=255)                                                                                              cTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGcc                                                       >  1:307059/1‑150 (MQ=255)                                                                                                  gTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGaaa                                                  <  1:217231/151‑1 (MQ=255)                                                                                                             gtgtGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGGTGCGGCGGGCCATAAAGTGCCAGAAGt                                       <  2:618486/151‑1 (MQ=255)                                                                                                             gtgtGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAg                                        >  1:96315/1‑150 (MQ=255)                                                                                                              tgtgAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTg                                      >  2:576332/1‑151 (MQ=255)                                                                                                               gtgAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTg                                      <  2:482397/150‑1 (MQ=255)                                                                                                               gtgAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTg                                      >  2:296563/1‑150 (MQ=255)                                                                                                                 gAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGaa                                    <  1:100532/150‑1 (MQ=255)                                                                                                                 gAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGaa                                    <  2:153189/150‑1 (MQ=255)                                                                                                                 gAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGGCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAAGGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGACCAGAAAGTGCCAGAAGTGaa                                    <  2:710364/150‑1 (MQ=255)                                                                                                                   aaGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATa                                 <  2:99631/151‑1 (MQ=255)                                                                                                                     gCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATAc                                >  1:381402/1‑150 (MQ=255)                                                                                                                     gCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACa                               <  1:292878/151‑1 (MQ=255)                                                                                                                       ccTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATc                             >  1:731000/1‑151 (MQ=255)                                                                                                                        cTGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCt                            >  1:550497/1‑151 (MQ=255)                                                                                                                         tGCTCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCtt                           >  2:80035/1‑151 (MQ=255)                                                                                                                            tCGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCg                         >  1:563545/1‑150 (MQ=255)                                                                                                                             cGGCGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCg                         <  1:439273/149‑1 (MQ=255)                                                                                                                                cGAGCGCGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTg                    >  1:595249/1‑151 (MQ=255)                                                                                                                                   gcgcGTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAAc                 >  1:285398/1‑151 (MQ=255)                                                                                                                                       gTGAAAGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCgg             >  1:369502/1‑151 (MQ=255)                                                                                                                                          aaaGATGTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATc          <  1:227363/151‑1 (MQ=255)                                                                                                                                                gTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTa     <  1:280283/150‑1 (MQ=255)                                                                                                                                                gTCCGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTAc    >  2:552587/1‑151 (MQ=255)                                                                                                                                                  ccGCCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCCGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTg  >  2:550437/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTCCGTCTGACTCACCGTCTGACCGATACGCCAGCGATCGTTTCGACCGACGCGGACGAAATGAGCACTCAGATGGCGAAACTGTTCGCTGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTG  >  NC_000913/496552‑496847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑