Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	648,555	A→G	R339R (CGT→CGC)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	648,555	0	A	G	100.0%	230.5 / NA	65	R339R (CGT→CGC)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/32);  total (33/32)

CGCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGT  >  NC_000913/648407‑648700                                                                                                                                                     |                                                                                                                                                  cGCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCg                                                                                                                                                 <  2:312181/151‑1 (MQ=255)  gCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCgg                                                                                                                                                >  2:576269/1‑151 (MQ=255)        agagCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGc                                                                                                                                          <  2:54558/151‑1 (MQ=255)        agagCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGc                                                                                                                                          >  2:264968/1‑151 (MQ=255)            ccGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGAtt                                                                                                                                      <  1:464179/151‑1 (MQ=255)              ggTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTgc                                                                                                                                    <  1:479413/151‑1 (MQ=255)                tCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTgcgc                                                                                                                                  >  1:84627/1‑151 (MQ=255)                 cAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCgca                                                                                                                                 <  2:354177/151‑1 (MQ=255)                  aGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCgcag                                                                                                                                <  2:548847/151‑1 (MQ=255)                      cGTTAACGTTGAACTGGGCGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGCTGCGGGCCAGCGATTGCGCAGCCTg                                                                                                                            >  2:175647/1‑151 (MQ=255)                          aaCGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCt                                                                                                                         <  2:732105/150‑1 (MQ=255)                          aaCGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTg                                                                                                                        >  2:634971/1‑151 (MQ=255)                                    tGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGctct                                                                                                              >  1:151748/1‑151 (MQ=255)                                    tGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGctc                                                                                                               >  2:309099/1‑150 (MQ=255)                                     gggTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTg                                                                                                             >  2:524682/1‑151 (MQ=255)                                           cAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACAt                                                                                                        <  1:125318/150‑1 (MQ=255)                                              tttCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATcc                                                                                                      >  1:414484/1‑149 (MQ=255)                                              tttCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATcc                                                                                                      >  1:755296/1‑149 (MQ=255)                                                 ccAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCcagcag                                                                                                 <  2:719282/151‑1 (MQ=255)                                                    gcgcgcTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGttt                                                                                              <  1:696875/151‑1 (MQ=255)                                                        gcTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCgg                                                                                          >  1:218847/1‑151 (MQ=255)                                                          tCAGTACCACCACGTCGCGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGa                                                                                         >  2:423309/1‑150 (MQ=255)                                                          tCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGAt                                                                                        >  1:311332/1‑151 (MQ=255)                                                           cAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGAt                                                                                        <  1:103945/150‑1 (MQ=255)                                                           cAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGAt                                                                                        <  1:446024/150‑1 (MQ=255)                                                           cAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATc                                                                                       <  2:12164/151‑1 (MQ=255)                                                           cAGTACCACCACGGCGAGACGAGCAACCGAGGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATc                                                                                       <  2:173339/151‑1 (MQ=255)                                                              tACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATc                                                                                       >  2:547591/1‑148 (MQ=255)                                                               accaccACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGAc                                                                                   <  2:554676/151‑1 (MQ=255)                                                                ccaccaCGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGAcc                                                                                  <  2:189946/151‑1 (MQ=255)                                                                 caccacGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCt                                                                                 <  2:522633/151‑1 (MQ=255)                                                                     acGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCttttt                                                                             <  2:673160/151‑1 (MQ=255)                                                                      cGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTc                                                                            <  2:702041/151‑1 (MQ=255)                                                                          tagaCGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGCTGGCGCTGATTTCGATGTGATTGGGGTGGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTGTGCGGATCAGACATTTTTCGt                                                                          >  2:544782/2‑149 (MQ=255)                                                                             aCGATCAACCGATGCGCCTTTCGATCCCCAGTTAGCGTGCTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGCCCTTTTTCGTGCAg                                                                      <  2:139508/150‑1 (MQ=255)                                                                                aTCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCa                                                                  <  1:692188/151‑1 (MQ=255)                                                                                   aaCCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAAcc                                                               >  1:348736/1‑151 (MQ=255)                                                                                      cGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc                                                            >  2:638752/1‑151 (MQ=255)                                                                                          gcgcCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgtc                                                         <  2:726988/150‑1 (MQ=255)                                                                                           cgcCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc                                                       >  1:731266/1‑151 (MQ=255)                                                                                           cgcCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc                                                       >  2:461348/1‑151 (MQ=255)                                                                                            gcCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgcg                                                      >  2:197125/1‑151 (MQ=255)                                                                                              cTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt                                                    >  2:628889/1‑151 (MQ=255)                                                                                                   gAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAc                                               >  2:68330/1‑151 (MQ=255)                                                                                                    aaCCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAcc                                              <  1:747002/151‑1 (MQ=255)                                                                                                    aaCCCCAGTTAGCGTACTGGTTGGCGCTGATTTAGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAcc                                              >  2:333739/1‑151 (MQ=255)                                                                                                     aCCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCg                                             <  1:614544/151‑1 (MQ=255)                                                                                                      ccccAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGc                                            <  2:600555/151‑1 (MQ=255)                                                                                                           gTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAGCCATCGTCGCGGTAATACCGCCAAgg                                       <  2:359158/151‑1 (MQ=255)                                                                                                             tAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGc                                     >  2:68364/1‑151 (MQ=255)                                                                                                              aGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCg                                    >  1:594271/1‑151 (MQ=255)                                                                                                                  tACTGGTTGGGGCTGATTTCGTTGTGATTGGGGTTGCGGGCGAGCGATTGCGCAGCATGGCTGTTAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACGTTTTTCGTGCAGGTCAACCATCGTCGCGGTACTTCCGCCAAGGGCGa                                   <  2:407240/148‑1 (MQ=255)                                                                                                                     tGGTTGGCGCTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCgg                             >  1:235235/1‑151 (MQ=255)                                                                                                                           gcgcTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGaa                       <  1:189859/151‑1 (MQ=255)                                                                                                                             gcTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAat                      >  1:185966/1‑150 (MQ=255)                                                                                                                             gcTGATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAat                      >  1:417677/1‑150 (MQ=255)                                                                                                                                gATTTCGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcg                  >  1:420390/1‑151 (MQ=255)                                                                                                                                     cGATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCt             <  1:305720/151‑1 (MQ=255)                                                                                                                                      gATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCt             >  1:610040/1‑150 (MQ=255)                                                                                                                                      gATGTGATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACAACCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGCCACCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCTAATAGCGCGGc              <  2:219643/149‑1 (MQ=255)                                                                                                                                        tgtgAGTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGTTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCGAGGGCGAAGTCGGCGCGAATATCGCGGCTAc           <  2:54847/150‑1 (MQ=255)                                                                                                                                           gATTGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCa        <  1:461369/150‑1 (MQ=255)                                                                                                                                             ttGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCAttt     >  2:232523/1‑151 (MQ=255)                                                                                                                                             ttGGGGTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCAttt     >  1:520381/1‑151 (MQ=255)                                                                                                                                                gggTTGCGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGt  >  1:482229/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGCCGTCAGAGCCGGTCAGCACGTTAACGTTGAACTGGGTGTCAATTTCCAGCGCGCTCAGTACCACCACGTCGAGACGATCAACCGATGCGCCTTTCGAACCCCAGTTAGCGTACTGGTTGGCGCTGATTTCGATGTGATTGGGGTTACGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGT  >  NC_000913/648407‑648700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑