Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,250	C→T	V108I (GTA→ATA)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,250	0	C	T	100.0%	217.9 / NA	57	V108I (GTA→ATA)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/36);  total (21/36)

CGCCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGT  >  NC_000913/649103‑649382                                                                                                                                                    |                                                                                                                                     cgccgcTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCa                                                                                                                                    <  1:699450/150‑1 (MQ=255) cgccgcTCGGTCCCAGAGGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCa                                                                                                                                    <  2:361108/150‑1 (MQ=255)   ccgcTCTGTCCCAGAGGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgc                                                                                                                                  <  2:318361/150‑1 (MQ=255)   ccgcTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcg                                                                                                                                 >  1:429877/1‑151 (MQ=255)   ccgcTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAg                                                                                                                                   <  2:694248/149‑1 (MQ=255)      ctctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggcg                                                                                                                              >  1:243373/1‑151 (MQ=255)      ctctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggc                                                                                                                               <  1:736776/150‑1 (MQ=255)                 agccaccatccccccgTGATGGTGGATCTGCACCGGTTCCGCCAGCAGGCCACGGGGGATCTCTACCGGCAGTGGACCACGCAGGCCGGACGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCa                                                                                                                       <  2:83994/134‑1 (MQ=255)                 aTGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATc                                                                                                                    <  1:439495/150‑1 (MQ=255)                    cacaCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTc                                                                                                                <  1:122572/151‑1 (MQ=255)                      cacGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCa                                                                                                               <  1:10653/150‑1 (MQ=255)                       acGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAgg                                                                                                             >  1:80124/1‑151 (MQ=255)                         gACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCa                                                                                                               <  1:232532/147‑1 (MQ=255)                         gACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAggg                                                                                                            >  2:222463/1‑150 (MQ=255)                                    gagTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGcc                                                                                                 >  1:495766/1‑150 (MQ=255)                                    gagTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCa                                                                                                <  2:235226/151‑1 (MQ=255)                                     agTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGcc                                                                                                 >  2:375876/1‑149 (MQ=255)                                      gTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAgg                                                                                              <  2:590657/151‑1 (MQ=255)                                      gTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAgg                                                                                              <  1:696187/151‑1 (MQ=255)                                        ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAggg                                                                                             <  2:92426/150‑1 (MQ=255)                                              gCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGt                                                                                       <  1:544810/150‑1 (MQ=255)                                               cACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttt                                                                                     <  2:665151/151‑1 (MQ=255)                                                          ccAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCAt                                                                          >  2:179531/1‑151 (MQ=255)                                                           cagcagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATc                                                                         >  2:287271/1‑151 (MQ=255)                                                             gcagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCtt                                                                       <  1:445636/151‑1 (MQ=255)                                                             gcagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCtt                                                                       <  1:335503/151‑1 (MQ=255)                                                              cagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTc                                                                      <  2:369869/151‑1 (MQ=255)                                                              cagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTc                                                                      <  1:295705/151‑1 (MQ=255)                                                              cagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTc                                                                      <  1:135836/151‑1 (MQ=255)                                                               agACCACGGGAGATCTCGTCCGCCAGTGGAGAACGAAGGACGGAGGTATAAATGCGGGTAACCACGGCCTGGCGAATGGGTTCTATCAGCGGCGCATGGAAATTACTCAGGGAGGTGGACGCCAGGGTCAGGTTTTTAAAGTCCATCTTcg                                                                     >  2:144738/1‑151 (MQ=255)                                                                  ccACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgcg                                                                   >  1:520122/1‑150 (MQ=255)                                                                  ccACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgcg                                                                   <  2:303432/150‑1 (MQ=255)                                                                    aCGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATg                                                                >  2:111211/1‑151 (MQ=255)                                                                     cGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGa                                                               <  1:486870/151‑1 (MQ=255)                                                                      gggAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGAc                                                              <  1:35755/151‑1 (MQ=255)                                                                                 tCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCAcc                                                    >  1:116106/1‑150 (MQ=255)                                                                                        gTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGAc                                            <  2:722862/151‑1 (MQ=255)                                                                                             ccACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtc                                        >  2:11852/1‑150 (MQ=255)                                                                                                  cAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggtca                                  <  2:267670/151‑1 (MQ=255)                                                                                                  cAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggtca                                  <  1:65705/151‑1 (MQ=255)                                                                                                   aGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCAc                                 >  2:205832/1‑151 (MQ=255)                                                                                                    ggCCGGAGGTATAAATGCGGGTAACCACGCCCTGCCGCATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTACATCACCATATTGACGGTCAGGTCAcc                                >  2:49676/1‑151 (MQ=255)                                                                                                      ccGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGc                              <  1:484927/151‑1 (MQ=255)                                                                                                       cGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGcc                             >  2:262618/1‑151 (MQ=255)                                                                                                        ggAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCa                            <  1:11984/151‑1 (MQ=255)                                                                                                         gAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCAc                           >  2:657532/1‑151 (MQ=255)                                                                                                           ggTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACgg                         <  2:310801/151‑1 (MQ=255)                                                                                                           ggTATAAAGGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGTTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACg                          <  1:414272/150‑1 (MQ=255)                                                                                                                aaacgggggTAACCACGCCCTGGCGAAGGTGTTCTATAAGCGGCGTATGGCAATCACTCAGGGAGCGGGACGCCTGGGTCAGGTTTTTAAGGCCCATCTCCGCGAGGACCTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGc                    <  2:69822/145‑1 (MQ=255)                                                                                                                aaaTGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATAGTGACGGTCAGGTCACCGCCACGGAAAGc                    >  1:207111/1‑151 (MQ=255)                                                                                                                  aTGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGt                  <  1:428226/151‑1 (MQ=255)                                                                                                                     cGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGAt               <  1:573833/151‑1 (MQ=255)                                                                                                                      gggTATCCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTCCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGAt               <  2:185959/150‑1 (MQ=255)                                                                                                                         tAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATgga            >  2:468962/1‑150 (MQ=255)                                                                                                                           aCCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATggaagg         <  1:364723/151‑1 (MQ=255)                                                                                                                                 cccTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCg   >  2:714515/1‑151 (MQ=255)                                                                                                                                  ccTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGt  >  2:42976/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                     CGCCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGT  >  NC_000913/649103‑649382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑