Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	39,079	C→T	A13T (GCC→ACC)	caiB ←	crotonobetainyl CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	39,079	0	C	T	100.0%	201.5 / NA	53	A13T (GCC→ACC)	caiB	crotonobetainyl CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/31);  total (22/31)

GAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGCCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGATAAGACGCATCAGCGTC  >  NC_000913/38933‑39223                                                                                                                                                   |                                                                                                                                                 gagTTGCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCACAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAAc                                                                                                                                              >  2:117193/1‑151 (MQ=255)  agTTGCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACg                                                                                                                                             >  1:131423/1‑151 (MQ=255)     tGCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGcc                                                                                                                                          >  2:721116/1‑151 (MQ=255)      gCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCACTCCGGTCAACGGccc                                                                                                                                         >  1:18504/1‑151 (MQ=255)       cGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCg                                                                                                                                        <  1:232411/151‑1 (MQ=255)          gTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAAt                                                                                                                                     <  1:476077/151‑1 (MQ=255)                cGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGc                                                                                                                               >  2:378716/1‑151 (MQ=255)                cGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGc                                                                                                                               <  1:629239/151‑1 (MQ=255)                 ggTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGc                                                                                                                               >  2:143270/1‑150 (MQ=255)                    tGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATgg                                                                                                                           >  2:19379/1‑151 (MQ=255)                       aCGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGt                                                                                                                         >  2:743172/1‑150 (MQ=255)                        cgcgAATGGTGTCGGCCCAGTCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAg                                                                                                                       <  2:18504/151‑1 (MQ=255)                         gcgAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAga                                                                                                                      <  1:305900/151‑1 (MQ=255)                          cgAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAgat                                                                                                                     <  1:477586/151‑1 (MQ=255)                           gAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAgatg                                                                                                                    >  1:650412/1‑151 (MQ=255)                                gtgtCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCa                                                                                                               <  2:252970/151‑1 (MQ=255)                                    cGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCattat                                                                                                           <  1:721160/151‑1 (MQ=255)                                    cGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCattat                                                                                                           <  1:129001/151‑1 (MQ=255)                                    cGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCatta                                                                                                            <  1:552241/150‑1 (MQ=255)                                       cccAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTg                                                                                                        <  1:32349/151‑1 (MQ=255)                                         cAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCt                                                                                                      <  2:165881/151‑1 (MQ=255)                                           ggCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTcc                                                                                                    <  2:709845/151‑1 (MQ=255)                                            gCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCt                                                                                                   >  2:149696/1‑151 (MQ=255)                                               aCGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGa                                                                                                 >  1:496005/1‑150 (MQ=255)                                                 gTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGaaaa                                                                                              <  1:293740/151‑1 (MQ=255)                                                     tcGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAAttt                                                                                          >  1:106985/1‑151 (MQ=255)                                                      cGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGGAAAAttt                                                                                          <  2:112431/150‑1 (MQ=255)                                                      cGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAAttt                                                                                          <  2:281066/150‑1 (MQ=255)                                                      cGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCACGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTa                                                                                         <  2:253471/151‑1 (MQ=255)                                                          ccAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGt                                                                                      >  1:99280/1‑150 (MQ=255)                                                                   ttCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGAc                                                                            >  2:734567/1‑151 (MQ=255)                                                                     ccGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACt                                                                           <  1:563670/150‑1 (MQ=255)                                                                      cgcgCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGc                                                                         <  1:139299/151‑1 (MQ=255)                                                                       gcgcCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGcc                                                                        <  1:438885/151‑1 (MQ=255)                                                                           cccATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACTCGCAATCCGGTCAACGGCCCGAATTTCGGCTGGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGcc                                                                        >  2:63664/1‑147 (MQ=255)                                                                             cATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATgc                                                                  <  1:540589/151‑1 (MQ=255)                                                                                tCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATgcgg                                                                <  1:751214/150‑1 (MQ=255)                                                                                       aCATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACg                                                        >  2:620643/1‑151 (MQ=255)                                                                                          tttGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCtt                                                     >  2:523166/1‑151 (MQ=255)                                                                                            tGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCttt                                                    <  1:32671/150‑1 (MQ=255)                                                                                             gCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTAt                                                  <  1:204851/151‑1 (MQ=255)                                                                                              cccGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATc                                                 >  2:499924/1‑151 (MQ=255)                                                                                                   cAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGcc                                            >  1:40347/1‑151 (MQ=255)                                                                                                    aaaCGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCt                                           >  1:422213/1‑151 (MQ=255)                                                                                                    aaaCGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCt                                           >  2:326466/1‑151 (MQ=255)                                                                                                     aaCGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTa                                          <  1:315909/151‑1 (MQ=255)                                                                                                        ggTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACa                                        <  2:659818/150‑1 (MQ=255)                                                                                                           ccGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGACGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTc                                     >  2:143511/1‑150 (MQ=255)                                                                                                                         aCCGGAGAAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGc                       <  1:206372/150‑1 (MQ=255)                                                                                                                             gagaAGACAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGAt                  <  1:720772/151‑1 (MQ=255)                                                                                                                              agaagaCAACGCGCAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGAt                  <  1:657714/150‑1 (MQ=255)                                                                                                                                           cAATCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGATAAGACGCATCAGCg    <  2:102026/151‑1 (MQ=255)                                                                                                                                             aTCCGGTCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGATAAGACGCATCAGCGTc  <  2:745882/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGTCGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGCCAACGGCCCGAATTTCGGCATGGGTAGATGATCCATTATTTGCTCCTGAAAAATTTATGTAGCGCATGACTGCCGGATGCGGCGTAAACGCTTTATCCGGCCTACATTCGTGCTCCCGTAGGCCTGATAAGACGCATCAGCGTC  >  NC_000913/38933‑39223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑