Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,679	A→G	V271A (GTG→GCG)	citE ←	citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,679	0	A	G	100.0%	242.7 / NA	68	V271A (GTG→GCG)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/30);  total (38/30)

ACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTT  >  NC_000913/649531‑649826                                                                                                                                                     |                                                                                                                                                    aCCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACcgcg                                                                                                                                                   >  1:746930/1‑151 (MQ=255)     gTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGa                                                                                                                                               <  2:641619/151‑1 (MQ=255)     gTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGa                                                                                                                                               <  1:569262/151‑1 (MQ=255)        cTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGc                                                                                                                                            >  2:172129/1‑151 (MQ=255)          tGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAgg                                                                                                                                             <  1:491765/148‑1 (MQ=255)           gTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAGGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCtt                                                                                                                                         >  2:511034/1‑151 (MQ=255)            tCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTc                                                                                                                                        >  1:38884/1‑151 (MQ=255)             cGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTc                                                                                                                                        <  2:287116/150‑1 (MQ=255)              gTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTcgc                                                                                                                                      >  2:728680/1‑151 (MQ=255)                  agaTTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAgc                                                                                                                                  >  1:765101/1‑151 (MQ=255)                   gaTTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAgcg                                                                                                                                 >  1:733891/1‑151 (MQ=255)                     ttGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAgcggc                                                                                                                               >  1:525279/1‑151 (MQ=255)                           aaTTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCtt                                                                                                                          >  2:62920/1‑150 (MQ=255)                                tCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCgg                                                                                                                    >  1:734013/1‑151 (MQ=255)                                     gTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCt                                                                                                               <  1:309808/151‑1 (MQ=255)                                     gTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCt                                                                                                               >  2:533227/1‑151 (MQ=255)                                     gTCATTTTGATTGCCATATTCTGCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGCGCGCGGTCGATAACCGGACCGTCCACCATCTTGCCGTTGAGGCAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCtt                                                                                                                 <  2:70920/149‑1 (MQ=255)                                              aTTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcgg                                                                                                      >  1:478082/1‑151 (MQ=255)                                                tGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGAcgccg                                                                                                      >  2:391684/1‑147 (MQ=255)                                                      aTTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGt                                                                                               <  2:746885/150‑1 (MQ=255)                                                         cttcGCGGATTCCGGAAAGTTGTGCACGGGAGAGCACCAGACGGGCGCGATCGCTAACCGGACCATCCACCATCTTGCCGTTCAGGGAAACCGCGCCGATAACTTCGCGTGCGGCGGCTATAGCGGCTTATAAGTCGAGGCCGGCGAGAt                                                                                            >  2:509106/1‑150 (MQ=255)                                                         cttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGGGt                                                                                               >  2:297930/1‑147 (MQ=255)                                                         cttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGAt                                                                                            >  2:281117/1‑150 (MQ=255)                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAc                                                                                        >  1:438232/1‑151 (MQ=255)                                                             gcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCggcgggc                                                                                                 >  2:688494/1‑141 (MQ=255)                                                                gATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCActtct                                                                                    <  1:452002/151‑1 (MQ=255)                                                                    ccGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttctttct                                                                                <  1:696340/151‑1 (MQ=255)                                                                     cGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttctttct                                                                                <  2:382442/150‑1 (MQ=255)                                                                     cGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttcttt                                                                                  >  1:306173/1‑148 (MQ=255)                                                                        aaaGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGt                                                                            <  2:243364/151‑1 (MQ=255)                                                                           gTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTTGGTCgg                                                                         >  2:545021/1‑151 (MQ=255)                                                                              cTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGt                                                                        <  2:502858/149‑1 (MQ=255)                                                                              cTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTg                                                                       >  1:216688/1‑150 (MQ=255)                                                                                gCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCg                                                                     <  1:693516/150‑1 (MQ=255)                                                                                gCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGt                                                                    <  1:129486/151‑1 (MQ=255)                                                                                gCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGt                                                                    <  1:62921/151‑1 (MQ=255)                                                                                  aCGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTag                                                                  <  2:391028/151‑1 (MQ=255)                                                                                    gggAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGGGCGTa                                                                   >  2:264879/1‑148 (MQ=255)                                                                                       agagCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGGCGGGGCGGAGAgg                                                               >  1:506823/1‑149 (MQ=255)                                                                                         agCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAgggt                                                             >  1:267707/1‑147 (MQ=255)                                                                                           cACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgc                                                         <  2:586749/151‑1 (MQ=255)                                                                                              cAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgcagc                                                      <  1:745616/151‑1 (MQ=255)                                                                                                 aCGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGa                                                   <  1:215256/151‑1 (MQ=255)                                                                                                  cGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgcagc                                                      >  1:366502/1‑147 (MQ=255)                                                                                                         gatcgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGCCGGTGCGCAGAGGTTGTGCAGCCGATCAAt                                              >  1:14600/1‑148 (MQ=255)                                                                                                           tcgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCCCTTCTTTCTGGGTCGGGGCGGAGAGGTTGTGCAGCAGATCAATCTGa                                          >  2:439427/1‑150 (MQ=255)                                                                                                            cgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGAcg                                        <  2:686984/151‑1 (MQ=255)                                                                                                             gatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGAcgc                                       >  1:247216/1‑151 (MQ=255)                                                                                                              atAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGGTCCACCTCTTTCTTGGTCGGTGCGTAGAGGGTGTGCAGCAGATCAATCt                                            >  2:232302/1‑145 (MQ=255)                                                                                                                aaCCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCggg                                    <  1:728724/151‑1 (MQ=255)                                                                                                                 aCCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGt                                   <  1:557682/151‑1 (MQ=255)                                                                                                                  ccGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGTTCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGtt                                  <  1:222472/151‑1 (MQ=255)                                                                                                                  ccGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGtt                                  <  2:625459/151‑1 (MQ=255)                                                                                                                  ccGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGtt                                  <  2:528623/151‑1 (MQ=255)                                                                                                                     gACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGTATCAGCGGCTTCTACGACGCGGCGGGCGTGAACCACTTCCTTCTGGGTCGGAGCGTAGAGGTTGTGCAGCAGATCAATATGAAGCGGGTTGa                                >  1:373959/1‑150 (MQ=255)                                                                                                                      aCCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCCAGGCCTTCGCGAGCGGCGGCTTCCGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTCTTTCTGGGGCGGCGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGGTGATc                              >  2:373008/1‑151 (MQ=255)                                                                                                                       ccGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCTTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCa                             <  1:23379/151‑1 (MQ=255)                                                                                                                       ccGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCa                             <  1:24811/151‑1 (MQ=255)                                                                                                                            caccaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTGCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGAt                        <  2:520098/151‑1 (MQ=255)                                                                                                                             accaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCt                                            <  2:320308/130‑1 (MQ=255)                                                                                                                             accaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGGTGTGGAGCAGATCAATCt                                            >  1:320321/1‑130 (MQ=255)                                                                                                                               caTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGAttt                      >  1:493762/1‑150 (MQ=255)                                                                                                                                 tCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGGGCGGAGGGGGTGTGCAGCAGATCAATCTGACGCGGGGTGGTTAGCGCTTTTcc                   >  2:257130/1‑151 (MQ=255)                                                                                                                                   ttGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCg                  >  1:689308/1‑150 (MQ=255)                                                                                                                                     gCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTc                <  1:533253/150‑1 (MQ=255)                                                                                                                                        gTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTTTTTTTCGGACGGTGCGTAGAGGGTGTGCAGCCGATCAACTTGACGCCGGTTGATCAGCGCTTTGCCGTCaa              >  2:208344/1‑149 (MQ=255)                                                                                                                                          tCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCGGATCAATCTGACGCGGGTTGATCCGCGGTTTGCCGTCAAAAcc          >  1:434929/1‑151 (MQ=255)                                                                                                                                             gggAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGGCGCGGGTTGATCAGCGATTTGCCGTCAAAGccc         >  1:129446/1‑149 (MQ=255)                                                                                                                                                  aCCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGtt  >  1:2974/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTT  >  NC_000913/649531‑649826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑