Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	671,974	G→A	D71D (GAC→GAT)	lptE ←	LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	671,974	0	G	A	100.0%	231.8 / NA	59	D71D (GAC→GAT)	lptE	LPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

TGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACG  >  NC_000913/671826‑672117                                                                                                                                                     |                                                                                                                                                tGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATc                                                                                                                                               >  1:308640/1‑151 (MQ=255)           cGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTg                                                                                                                                     <  1:369828/151‑1 (MQ=255)                        cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCa                                                                                                                         >  2:278411/1‑150 (MQ=255)                         ggCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCa                                                                                                                         >  1:719582/1‑149 (MQ=255)                          gCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGc                                                                                                                      >  1:761658/1‑151 (MQ=255)                             ggggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAAc                                                                                                                   >  2:515315/1‑151 (MQ=255)                              gggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACt                                                                                                                  <  2:446422/151‑1 (MQ=255)                              gggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACt                                                                                                                  >  1:402956/1‑151 (MQ=255)                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTc                                                                                                                 <  1:559832/151‑1 (MQ=255)                                   gaaCGCGGTCGCGTTAACCGTCATGATCATCTGATGATCTGCTGTTTGACCGTTACGGAGTACCGATGCGTGATCTTTAGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCGTACGCGTGGTTTCTTTATCGAGCAACTCGaca                                                                                                             <  2:731816/150‑1 (MQ=255)                                     acacGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAcc                                                                                                           >  2:380527/1‑151 (MQ=255)                                     acacGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAcc                                                                                                           >  1:485529/1‑151 (MQ=255)                                       acGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTgg                                                                                                                                    <  1:335684/124‑1 (MQ=255)                                       acGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTgg                                                                                                                                    >  2:335670/1‑124 (MQ=255)                                            cGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAg                                                                                                     <  2:274330/150‑1 (MQ=255)                                             gCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAc                                                                                                   <  1:399800/151‑1 (MQ=255)                                                 tAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                                                                                                >  1:110294/1‑150 (MQ=255)                                                  aaCCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc                                                                                              >  2:46335/1‑151 (MQ=255)                                                     cGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTgg                                                                                           >  1:322948/1‑151 (MQ=255)                                                      gTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGt                                                                                          >  1:131111/1‑151 (MQ=255)                                                       tCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGtt                                                                                         >  1:150625/1‑151 (MQ=255)                                                                   gATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccg                                                                                 <  2:218497/147‑1 (MQ=255)                                                                      aCTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGc                                                                           <  1:135398/150‑1 (MQ=255)                                                                      aCTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCt                                                                          >  1:596565/1‑151 (MQ=255)                                                                        tctGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCtt                                                                         >  2:374563/1‑150 (MQ=255)                                                                          tgctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAAt                                                                      >  1:113797/1‑151 (MQ=255)                                                                           gctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAAt                                                                      <  1:420105/150‑1 (MQ=255)                                                                            ctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATgg                                                                    >  2:181052/1‑151 (MQ=255)                                                                              gTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGcc                                                                  >  2:471053/1‑151 (MQ=255)                                                                              gTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGcc                                                                  <  2:71157/151‑1 (MQ=255)                                                                                        tACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGAt                                                         <  1:385720/150‑1 (MQ=255)                                                                                         aCGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCg                                                       <  2:573851/151‑1 (MQ=255)                                                                                          cGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGc                                                      >  1:625886/1‑151 (MQ=255)                                                                                           ggAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGcc                                                     >  1:186453/1‑151 (MQ=255)                                                                                           ggAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGcc                                                     <  1:616171/151‑1 (MQ=255)                                                                                           ggAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGcc                                                     >  1:741843/1‑151 (MQ=255)                                                                                                aCCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGt                                                <  2:346818/151‑1 (MQ=255)                                                                                                aCCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGt                                                <  1:211006/151‑1 (MQ=255)                                                                                                    aTGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGTATCGCCTGAGTCCAg                                            <  1:464829/151‑1 (MQ=255)                                                                                                     tGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAgg                                           >  2:563335/1‑151 (MQ=255)                                                                                                      gCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGa                                          <  2:612132/151‑1 (MQ=255)                                                                                                       cGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGAt                                         <  2:277176/151‑1 (MQ=255)                                                                                                                tCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCtt                                >  2:284903/1‑151 (MQ=255)                                                                                                                 cgcgATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCtt                                <  1:380548/150‑1 (MQ=255)                                                                                                                   cgATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCAt                             >  1:365860/1‑151 (MQ=255)                                                                                                                    gATGCTCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCAt                             >  1:521165/1‑150 (MQ=255)                                                                                                                         tCACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTgg                        >  1:517081/1‑150 (MQ=255)                                                                                                                          cACTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTgga                       >  1:259971/1‑150 (MQ=255)                                                                                                                           aCTTTACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTggaag                     <  1:474770/151‑1 (MQ=255)                                                                                                                             tttACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTggaagg                    >  1:635494/1‑150 (MQ=255)                                                                                                                               tACCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAAc                 >  1:234940/1‑151 (MQ=255)                                                                                                                                aCCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAAcc                <  1:225241/151‑1 (MQ=255)                                                                                                                                aCCCAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAAcc                <  1:663085/151‑1 (MQ=255)                                                                                                                                  ccAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTg              <  1:351822/151‑1 (MQ=255)                                                                                                                                   cAAACGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAATGAACCtac             <  2:141828/151‑3 (MQ=255)                                                                                                                                    aaaCGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCg            <  2:277566/151‑1 (MQ=255)                                                                                                                                    aaaCGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCg            <  2:191047/151‑1 (MQ=255)                                                                                                                                    aaaCGCAAGGATGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCg            >  2:617964/1‑151 (MQ=255)                                                                                                                                              aTGGAACATCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACg  >  1:369948/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                TGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACG  >  NC_000913/671826‑672117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑