Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,748	T→C	K283E (AAG→GAG)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,748	0	T	C	100.0%	137.8 / NA	37	K283E (AAG→GAG)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/14);  total (23/14)

TGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAAT  >  NC_000913/700604‑700895                                                                                                                                                 |                                                                                                                                                    tGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAAc                                                                                                                                                 >  2:292585/1‑149 (MQ=255)   aTAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAAc                                                                                                                                                 >  2:470330/1‑147 (MQ=255)             cGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCg                                                                                                                                   >  1:124410/1‑151 (MQ=255)               aTGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCgtt                                                                                                                                 <  2:735716/151‑1 (MQ=255)                  gTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCgttgtt                                                                                                                              <  1:178237/151‑1 (MQ=255)                         ccAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGc                                                                                                                       >  1:597497/1‑151 (MQ=255)                           aGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAg                                                                                                                     <  1:649895/151‑1 (MQ=255)                               gacgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGtt                                                                                                                 <  1:292046/151‑1 (MQ=255)                                     cgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGc                                                                                                           <  2:691809/151‑1 (MQ=255)                                       acATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCg                                                                                                          >  1:556278/1‑150 (MQ=255)                                          tACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAt                                                                                                        >  2:39968/1‑149 (MQ=255)                                           aCTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGt                                                                                                     <  2:619983/151‑1 (MQ=255)                                                       ccGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGc                                                                                          <  1:379663/150‑1 (MQ=255)                                                         gACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaa                                                                                       <  1:536906/151‑1 (MQ=255)                                                                 aCTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCg                                                                               <  1:420883/151‑1 (MQ=255)                                                                   tATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAATGTTGCCg                                                                               <  2:349070/149‑1 (MQ=255)                                                                   tATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGc                                                                              >  2:660784/1‑150 (MQ=255)                                                                       gCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTgg                                                                         >  2:670932/1‑151 (MQ=255)                                                                          tttGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGc                                                                        >  1:728932/1‑149 (MQ=255)                                                                             gTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGt                                                                   >  2:309534/1‑151 (MQ=255)                                                                              ttCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGtt                                                                  >  2:630561/1‑151 (MQ=255)                                                                               tCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTc                                                                 >  1:720073/1‑151 (MQ=255)                                                                                 gcgGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCAc                                                               >  1:674413/1‑151 (MQ=255)                                                                                   ggCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCAccc                                                             >  2:396693/1‑151 (MQ=255)                                                                                   ggCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCAccc                                                             >  2:701959/1‑151 (MQ=255)                                                                                    gCTTCGCTGATAGTTTTGAAGGTGCAGTCGTCCAGCGGCACGCGCCTTTTGTAACCCGGATCTAACAGATTCAACACCCGATGGTCAATGGAAGCGGGGGCAGCTATAGCTTCCAGGCAGACAAAGCTGCCg                                                                               >  2:651558/1‑132 (MQ=255)                                                                                        tGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCgg                                                        >  1:377507/1‑151 (MQ=255)                                                                                               aGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                 >  1:162480/1‑151 (MQ=255)                                                                                                  tttGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                               <  2:721185/150‑1 (MQ=255)                                                                                                     gATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                            <  1:491460/150‑1 (MQ=255)                                                                                                                      gCGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAc                          >  1:707827/1‑151 (MQ=255)                                                                                                                       cGGCACGCGGCTCTGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcg                         >  1:215984/1‑151 (MQ=255)                                                                                                                                   ctGGTAGCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgttgc               <  2:62029/149‑1 (MQ=255)                                                                                                                                        aGCCCTGCTCTAAGAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAAt        >  1:229629/1‑151 (MQ=255)                                                                                                                                         gCCCTGCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAAt        >  2:85999/1‑150 (MQ=255)                                                                                                                                              gCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa   <  2:492615/150‑1 (MQ=255)                                                                                                                                              gCTCTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAAt  >  2:601416/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAAT  >  NC_000913/700604‑700895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑