Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	914,373	A→G	V162A (GTC→GCC)	ybjE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	914,373	0	A	G	100.0%	161.9 / NA	42	V162A (GTC→GCC)	ybjE	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/25);  total (17/25)

ACCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGACAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGC  >  NC_000913/914228‑914520                                                                                                                                                  |                                                                                                                                                    aCCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCa                                                                                                                                                   >  2:86490/1‑148 (MQ=255) aCCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCa                                                                                                                                                   <  1:86491/148‑1 (MQ=255)    ggAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGc                                                                                                                                             <  2:613826/151‑1 (MQ=255)     gAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCt                                                                                                                                            <  1:345515/151‑1 (MQ=255)      aaaGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCtt                                                                                                                                           <  1:701218/151‑1 (MQ=255)      aaaGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCt                                                                                                                                            <  2:766608/150‑1 (MQ=255)             aTACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTc                                                                                                                                    <  2:765540/151‑1 (MQ=255)                ccAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATg                                                                                                                                 >  1:463072/1‑151 (MQ=255)                            ggAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTgcgc                                                                                                                     >  1:134854/1‑151 (MQ=255)                              aGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCaa                                                                                                                   <  2:48332/151‑1 (MQ=255)                                  cATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGa                                                                                                               <  1:755989/151‑1 (MQ=255)                                      gCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTTCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATAc                                                                                                           >  1:115959/1‑151 (MQ=255)                                        cAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCa                                                                                                         <  2:291664/151‑1 (MQ=255)                                            gcgGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGa                                                                                                     <  2:662526/151‑1 (MQ=255)                                             cgGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACg                                                                                                      >  1:618866/1‑149 (MQ=255)                                                                  gAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACgt                                                                               >  2:460052/1‑151 (MQ=255)                                                                   aaTAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACgtg                                                                              <  1:296928/151‑1 (MQ=255)                                                                      aaaGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTAt                                                                           <  1:42738/151‑1 (MQ=255)                                                                        aGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTc                                                                         <  1:440083/151‑1 (MQ=255)                                                                             ttaattaaACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTgg                                                                    >  1:16533/1‑151 (MQ=255)                                                                                attaaACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCtt                                                                 <  1:134350/151‑1 (MQ=255)                                                                                   aaaCCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTc                                                                <  2:170600/149‑1 (MQ=255)                                                                                   aaaCCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCgg                                                              <  1:166553/151‑1 (MQ=255)                                                                                         ccaATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCgt                                                        <  1:608985/151‑1 (MQ=255)                                                                                          caATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCgtg                                                       >  1:154689/1‑151 (MQ=255)                                                                                            aTTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGtgtt                                                     >  1:406518/1‑151 (MQ=255)                                                                                                    aCTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGaaa                                             <  2:424880/151‑1 (MQ=255)                                                                                                        aCAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCa                                         <  2:480051/151‑1 (MQ=255)                                                                                                          aaCCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGt                                       >  2:136334/1‑151 (MQ=255)                                                                                                           accaccaccACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTc                                      <  2:126263/151‑1 (MQ=255)                                                                                                             caccaccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTcc                                     >  1:89719/1‑150 (MQ=255)                                                                                                              accaccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCAc                                   >  1:729858/1‑151 (MQ=255)                                                                                                                  ccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAg                               <  1:249599/151‑1 (MQ=255)                                                                                                                     cGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGAcc                            <  1:288056/151‑1 (MQ=255)                                                                                                                        cGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAAt                         >  1:43979/1‑151 (MQ=255)                                                                                                                          aCAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATgg                       >  2:36223/1‑151 (MQ=255)                                                                                                                            aaTCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCa                     <  2:167735/151‑1 (MQ=255)                                                                                                                                   ccccGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCa               <  1:108269/150‑1 (MQ=255)                                                                                                                                   ccccGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCa               <  2:133215/150‑1 (MQ=255)                                                                                                                                       ggCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCac          >  1:753201/1‑151 (MQ=255)                                                                                                                                            ttAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCactact      >  1:222228/1‑150 (MQ=255)                                                                                                                                               aGGGCAATCTGCTTTAAGGACATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGCAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTAcgc  >  1:54841/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGACAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGC  >  NC_000913/914228‑914520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑