Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,431	A→G	S42P (TCC→CCC)	pflB ←	formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,431	0	A	G	100.0%	150.7 / NA	43	S42P (TCC→CCC)	pflB	formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

TTGATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGT  >  NC_000913/953283‑953575                                                                                                                                                     |                                                                                                                                                 ttgATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCt                                                                                                                                                >  1:4842/1‑151 (MQ=255)        aGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCTCCAGCCAGTAAGGGCTCGTCAcc                                                                                                                                         <  2:449668/151‑1 (MQ=255)          ccAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCt                                                                                                                                       >  2:194761/1‑151 (MQ=255)           cAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTc                                                                                                                                      <  2:339152/151‑1 (MQ=255)             tcgtcgtGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGt                                                                                                                                    >  1:395580/2‑151 (MQ=255)                           gATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCGGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGttt                                                                                                                      >  2:632503/1‑151 (MQ=255)                              ggtggAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGttttt                                                                                                                    <  1:297253/150‑1 (MQ=255)                                tggAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCtg                                                                                                                 >  2:442601/1‑151 (MQ=255)                                       aaCAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCtgaatga                                                                                                            >  2:513708/1‑149 (MQ=255)                                          aGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCa                                                                                                       <  1:562359/151‑1 (MQ=255)                                           gCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCAc                                                                                                      >  2:734946/1‑151 (MQ=255)                                             ggTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACgg                                                                                                    >  2:330058/1‑151 (MQ=255)                                              gtgtCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACgg                                                                                                    >  1:406629/1‑150 (MQ=255)                                                      gTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACt                                                                                           <  2:571150/151‑1 (MQ=255)                                                        cAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTc                                                                                         <  1:596770/151‑1 (MQ=255)                                                          aCTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAt                                                                                       <  2:427810/151‑1 (MQ=255)                                                           cTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAtt                                                                                      >  2:592782/1‑151 (MQ=255)                                                           cTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAtt                                                                                      >  1:359891/1‑151 (MQ=255)                                                                  gTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAg                                                                               <  1:761447/151‑1 (MQ=255)                                                                   tgtgTGCGGATTTCCAGGTTAACGCCGTCCATTACTTTGTCCCACAGGGTGGAGTTCGCTTCAGTAGCGCCAGCCAGGAAGTGCTGATCACCCTCGTACGGAGTGTATTTTTCCTGACTAAAGTCACGAACGTTTTCTTCATTCTTCCAGt                                                                              >  2:420614/4‑151 (MQ=255)                                                                        gCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCAcc                                                                          <  1:451990/150‑1 (MQ=255)                                                                          ggTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCttt                                                                       >  2:443065/1‑151 (MQ=255)                                                                           gTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTg                                                                      <  2:646663/151‑1 (MQ=255)                                                                                    tttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCt                                                                                    >  2:322157/1‑128 (MQ=255)                                                                                    tttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCt                                                                                    <  1:322170/128‑1 (MQ=255)                                                                                     ttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCtt                                                            >  1:230212/1‑151 (MQ=255)                                                                                          gCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTccc                                                         >  1:629140/1‑149 (MQ=255)                                                                                                 aTTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTgg                                                <  1:51440/151‑1 (MQ=255)                                                                                                        tGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACtt                                         >  1:45142/1‑151 (MQ=255)                                                                                                         gTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACttt                                        >  2:662383/1‑151 (MQ=255)                                                                                                            ccACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTc                                      >  2:56810/1‑150 (MQ=255)                                                                                                              acaGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCAtt                                   >  2:34009/1‑151 (MQ=255)                                                                                                               caGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCAtt                                   >  1:481085/1‑150 (MQ=255)                                                                                                                aGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTaa                                 <  1:330071/151‑1 (MQ=255)                                                                                                                     ggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCg                            <  2:424453/151‑1 (MQ=255)                                                                                                                        ggtCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTCTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGa                          <  2:478592/150‑1 (MQ=255)                                                                                                                            gCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGt                     <  2:239794/151‑1 (MQ=255)                                                                                                                                cAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTaa                   <  2:684935/149‑1 (MQ=255)                                                                                                                                cAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAac                  <  2:580800/150‑1 (MQ=255)                                                                                                                                    aGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCGTTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACaccta             <  2:370605/151‑1 (MQ=255)                                                                                                                                          agccagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACcttct       >  1:321343/1‑151 (MQ=255)                                                                                                                                           gccagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACcttctt      <  2:321330/151‑1 (MQ=255)                                                                                                                                             cagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTaa    <  1:330685/151‑1 (MQ=255)                                                                                                                                               ggaagggCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGt  <  1:497013/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTGATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGT  >  NC_000913/953283‑953575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑