Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,109,893 C→T R187C (CGC→TGC)  opgG → OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,109,8930CT97.9% 184.6 / ‑3.2 48R187C (CGC→TGC) opgGOPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/17);  minor base A (0/1);  total (30/18)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGA  >  NC_000913/1109746‑1110037
                                                                                                                                                   |                                                                                                                                                
ccGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCt                                                                                                                                               >  1:59962/1‑151 (MQ=255)
    tCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCa                                                                                                                                            >  1:24906/1‑150 (MQ=255)
            aaaGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCt                                                                                                                                   >  2:721999/1‑151 (MQ=255)
             aaGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTg                                                                                                                                  <  1:543465/151‑1 (MQ=255)
              aGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTgg                                                                                                                                 >  1:374976/1‑151 (MQ=255)
                     aaCGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGc                                                                                                                          <  2:620157/151‑1 (MQ=255)
                           gAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCa                                                                                                                     >  2:15334/1‑150 (MQ=255)
                              aTCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAAc                                                                                                                 >  1:536659/1‑151 (MQ=255)
                               tCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAAcc                                                                                                                >  1:433942/1‑151 (MQ=255)
                                 gTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGa                                                                                                              <  1:723353/151‑1 (MQ=255)
                                  tCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGAc                                                                                                             >  1:692186/1‑151 (MQ=255)
                                  tCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGAc                                                                                                             >  2:51631/1‑151 (MQ=255)
                                            cGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACg                                                                                                    <  1:431063/150‑1 (MQ=255)
                                                         ttCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATg                                                                                      >  1:247/1‑151 (MQ=255)
                                                              cgTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTg                                                                                 >  1:493498/1‑151 (MQ=255)
                                                              cgTGATTGGTGCAGGGCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTg                                                                                 >  2:332708/1‑151 (MQ=255)
                                                                tGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCt                                                                               >  1:21238/1‑151 (MQ=255)
                                                                    tGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGa                                                                            <  2:534065/150‑1 (MQ=255)
                                                                      gTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTc                                                                         >  1:177792/1‑151 (MQ=255)
                                                                        gCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGAGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTcgc                                                                       <  2:254408/151‑1 (MQ=255)
                                                                           ggtcaggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTcgccgc                                                                    <  1:190700/151‑1 (MQ=255)
                                                                                ggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg                                                               <  1:398548/151‑1 (MQ=255)
                                                                                 gtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg                                                               >  1:641644/1‑150 (MQ=255)
                                                                                  tttATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  1:275768/1‑151 (MQ=255)
                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  2:592857/1‑149 (MQ=255)
                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  2:155696/1‑149 (MQ=255)
                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACa                                                            <  2:521234/150‑1 (MQ=255)
                                                                                      tGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAgg                                                          >  2:668470/1‑150 (MQ=255)
                                                                                         ccTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCt                                                      >  2:91412/1‑151 (MQ=255)
                                                                                             tCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTAc                                                   >  2:296604/1‑150 (MQ=255)
                                                                                             tCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTAc                                                   <  1:507655/150‑1 (MQ=255)
                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACa                                                  <  2:653950/148‑1 (MQ=255)
                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAAt                                               <  1:625870/151‑1 (MQ=255)
                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAAt                                               >  1:442061/1‑151 (MQ=255)
                                                                                                    gcgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGt                                           >  1:163904/1‑151 (MQ=255)
                                                                                                     cgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTg                                          <  2:746401/151‑1 (MQ=255)
                                                                                                      ggCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTgg                                         >  2:617648/1‑151 (MQ=255)
                                                                                                         cTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGtt                                       >  2:65280/1‑150 (MQ=255)
                                                                                                          tGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTAt                                     >  1:391202/1‑151 (MQ=255)
                                                                                                              aaTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGcc                                  >  1:304383/1‑150 (MQ=255)
                                                                                                              aaTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCa                                 <  1:342698/151‑1 (MQ=255)
                                                                                                               aTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGcc                                  >  2:370946/1‑149 (MQ=255)
                                                                                                                            tGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGacaca                     <  2:60780/149‑2 (MQ=255)
                                                                                                                             gCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGtt                  <  1:25990/151‑1 (MQ=255)
                                                                                                                             gCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGt                   <  2:640912/150‑1 (MQ=255)
                                                                                                                               cATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTtgt                <  1:741280/151‑1 (MQ=255)
                                                                                                                                              ttCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGa  >  2:618567/1‑150 (MQ=255)
                                                                                                                                              ttCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGa  >  1:104625/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGA  >  NC_000913/1109746‑1110037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑