Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,198,749	A→G	I19V (ATT→GTT)	lit →	T4 phage exclusion protein; cell death peptidase, e14 prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,198,749	0	A	G	100.0%	22.7 / NA	7	I19V (ATT→GTT)	lit	T4 phage exclusion protein; cell death peptidase, e14 prophage
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/2);  total (5/2)

ACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAC  >  NC_000913/1198622‑1198824                                                                                                                                |                                                                            aCATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCa                                                          >  2:562998/1‑147 (MQ=255)          aaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACg                                             >  2:165716/1‑151 (MQ=255)                       aTCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacgac                                 >  1:472550/1‑150 (MQ=255)                                 gTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATta                       <  1:563027/150‑1 (MQ=255)                                      tactacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAAttt                 >  1:284936/1‑151 (MQ=255)                                                                                        ttGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAc  >  1:205889/1‑116 (MQ=255)                                                                                        ttGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAc  <  2:205881/116‑1 (MQ=255)                                                                                                                                |                                                                            ACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAC  >  NC_000913/1198622‑1198824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑