Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,236,437	T→C	S269G (AGC→GGC)	ycgB ←	SpoVR family stationary phase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,236,437	0	T	C	100.0%	139.4 / NA	38	S269G (AGC→GGC)	ycgB	SpoVR family stationary phase protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

TTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCTCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTCGGCA  >  NC_000913/1236292‑1236583                                                                                                                                                   |                                                                                                                                                   ttGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCAcc                                                                                                                                                >  2:500792/1‑150 (MQ=255)    gtgtGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTAc                                                                                                                                            <  1:169007/151‑1 (MQ=255)         gCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGcaca                                                                                                                                       <  1:295689/151‑1 (MQ=255)           tgtgCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAAt                                                                                                                                     >  2:347324/1‑151 (MQ=255)            gtgCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATa                                                                                                                                    <  2:202799/151‑1 (MQ=255)              gCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACg                                                                                                                                  <  1:649196/151‑1 (MQ=255)                   aaCTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAgg                                                                                                                              >  1:600630/1‑150 (MQ=255)                    aCTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGAt                                                                                                                            <  2:399587/151‑1 (MQ=255)                             aaaacCGTCCCTTTATTTTCCCTCCACATTCCAGATGGTT‑AGGGTGGTGTTATGGCAAAAAGTCCCCCCGCCCTCCTTCCACCACC‑GGGTTTGTTTTTGCCGGTTAAAAAACTGGGCCACCTTACTCACAATACGCAGGATTTCACGCTGc                                                                                                                  <  2:385625/150‑1 (MQ=255)                                  cGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATg                                                                                                              >  1:265942/1‑151 (MQ=255)                                  cGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATg                                                                                                              >  2:646152/1‑151 (MQ=255)                                        gTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTcc                                                                                                         >  1:528464/1‑150 (MQ=255)                                        gTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCca                                                                                                        <  1:500816/151‑1 (MQ=255)                                           aCTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCcagca                                                                                                     <  1:121807/151‑1 (MQ=255)                                            cTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCcagc                                                                                                      >  2:605332/1‑149 (MQ=255)                                              ttCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCcagcag                                                                                                    <  2:8148/149‑1 (MQ=255)                                               tCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTg                                                                                                  <  2:219553/150‑1 (MQ=255)                                                      atcatACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCGCCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCAt                                                                                            <  2:241574/149‑1 (MQ=255)                                                         atACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTtc                                                                                       >  2:369442/1‑151 (MQ=255)                                                                 ggTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATaa                                                                                <  1:378991/150‑1 (MQ=255)                                                                       ggATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATagag                                                                         <  1:14867/151‑1 (MQ=255)                                                                       ggATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATagag                                                                         <  1:347338/151‑1 (MQ=255)                                                                           ggTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAgg                                                                     <  2:479450/151‑1 (MQ=255)                                                                               tAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGtt                                                                   >  1:233825/1‑149 (MQ=255)                                                                                 gTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCt                                                               >  2:338781/1‑151 (MQ=255)                                                                                    ccAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTtgt                                                            <  2:528439/151‑1 (MQ=255)                                                                                             cGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTcggac                                                   >  2:100890/1‑151 (MQ=255)                                                                                                    ccTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAgc                                            <  2:488792/151‑1 (MQ=255)                                                                                                        cGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAgcggcg                                        >  1:17707/1‑151 (MQ=255)                                                                                                          tt‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGgcgcg                                      >  2:753915/1‑151 (MQ=255)                                                                                                             catcaCCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCt                                    >  2:395205/1‑151 (MQ=255)                                                                                                              atcaCCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCtt                                   <  2:498093/151‑1 (MQ=255)                                                                                                                 aCCTGAGTCTGTTTTTGCGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAACATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTc                                  >  1:200856/1‑149 (MQ=255)                                                                                                                                  cGGATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTcc               >  2:740633/1‑151 (MQ=255)                                                                                                                                    gATAAAAATACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTc             <  2:673004/151‑1 (MQ=255)                                                                                                                                          aaTACTGGCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCtt       >  2:412297/1‑151 (MQ=255)                                                                                                                                                ggCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCTTCGCGCTTCg     >  1:305333/1‑147 (MQ=255)                                                                                                                                                ggCCCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTCGGCa  <  1:319883/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTT‑CATCACCTGAGTCTGTTTTTGCGGATAAAAATACTGGCTCACCTTACGCACAATACGCAGGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTCGGCA  >  NC_000913/1236292‑1236583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑