Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,682	A→G	V44A (GTA→GCA)	ychF ←	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,682	0	A	G	100.0%	107.2 / NA	32	V44A (GTA→GCA)	ychF	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/25);  total (7/25)

CTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCTT  >  NC_000913/1257546‑1257827                                                                                                                                         |                                                                                                                                                  cTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTc                                                                                                                                     >  2:404092/1‑151 (MQ=255)   ggTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCg                                                                                                                                    <  1:132398/150‑1 (MQ=255)      tACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTc                                                                                                                                >  1:422649/1‑151 (MQ=255)       aCCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCa                                                                                                                               <  2:214315/151‑1 (MQ=255)         ccAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAAt                                                                                                                             >  1:86801/1‑151 (MQ=255)          cAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATg                                                                                                                            <  2:16977/151‑1 (MQ=255)                ttCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGc                                                                                                                        >  2:460150/1‑149 (MQ=255)                 tCGCCTTTCGATTCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGGGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCa                                                                                                                       <  2:223682/149‑1 (MQ=255)                 tCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCa                                                                                                                       <  1:729183/149‑1 (MQ=255)                               gcCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCTGTTGATCCAGGCGAGGATCAGGCATTGGTGCGAGGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTGGGcc                                                                                                       <  2:48158/151‑1 (MQ=255)                                           aCCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCg                                                                                           <  2:637044/151‑1 (MQ=255)                                            ccGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCgg                                                                                          <  1:125175/151‑1 (MQ=255)                                                  atatCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTgg                                                                                    <  2:418421/151‑1 (MQ=255)                                                      cGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCa                                                                                 <  1:119397/150‑1 (MQ=255)                                                       gACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAgc                                                                               <  1:511947/151‑1 (MQ=255)                                                           aaTTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGt                                                                           <  2:554567/151‑1 (MQ=255)                                                                cATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAAc                                                                      <  2:476875/151‑1 (MQ=255)                                                                           gAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGAttt                                                           <  2:424043/151‑1 (MQ=255)                                                                               cGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAGTACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCg                                                       <  2:10800/151‑1 (MQ=255)                                                                                    gCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGtt                                                  <  2:612266/151‑1 (MQ=255)                                                                                          gTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCaa                                            <  1:310736/151‑1 (MQ=255)                                                                                          gTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCa                                             >  2:520563/1‑150 (MQ=255)                                                                                           ttttACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCaa                                            >  1:305774/1‑150 (MQ=255)                                                                                               aCGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcg                                        <  1:442774/150‑1 (MQ=255)                                                                                                cGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcgac                                      >  1:201428/1‑151 (MQ=255)                                                                                                    ttCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGAt                                   <  2:737990/150‑1 (MQ=255)                                                                                                     tCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATa                                  <  2:133203/150‑1 (MQ=255)                                                                                                            gTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCATGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGGTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATCCCGCAttt                          <  2:351998/151‑1 (MQ=255)                                                                                                               gATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGa                        <  2:351614/150‑1 (MQ=255)                                                                                                                      gCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATg                <  1:574460/151‑1 (MQ=255)                                                                                                                                   aTTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCt   <  1:224931/151‑1 (MQ=255)                                                                                                                                     tGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCtt  <  2:652208/150‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                  CTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCTT  >  NC_000913/1257546‑1257827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑