Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,391	T→C	T419A (ACA→GCA)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,391	0	T	C	100.0%	194.1 / NA	53	T419A (ACA→GCA)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/22);  total (31/22)

AGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCG  >  NC_000913/1307249‑1307538                                                                                                                                               |                                                                                                                                                    aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAAcc                                                                                                                                              <  1:557209/150‑1 (MQ=255)          cGGGTATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGtt                                                                                                                                    <  1:647922/151‑1 (MQ=255)               aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGcc                                                                                                                                >  1:165795/1‑150 (MQ=255)               aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCa                                                                                                                               <  1:742802/151‑1 (MQ=255)                   aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCg                                                                                                                            >  2:543767/1‑150 (MQ=255)                      aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCa                                                                                                                        <  2:766972/151‑1 (MQ=255)                       gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAg                                                                                                                       <  1:565572/151‑1 (MQ=255)                          gTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                                                                    <  2:350934/151‑1 (MQ=255)                          gTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                                                                    <  1:22364/151‑1 (MQ=255)                               cgcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctct                                                                                                               >  1:291406/1‑151 (MQ=255)                                gcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCtt                                                                                                              <  1:378430/151‑1 (MQ=255)                                   aaaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                                                           >  1:71829/1‑151 (MQ=255)                                                  gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCTCGCTCTTGGTATGCAGTAACCCg                                                                                              <  2:658707/149‑1 (MQ=255)                                                  gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCg                                                                                              <  1:35386/149‑1 (MQ=255)                                                   aaCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                                                           >  1:362488/1‑151 (MQ=255)                                                         ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                                                                     >  2:590360/1‑151 (MQ=255)                                                          gcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACt                                                                                    <  1:575669/151‑1 (MQ=255)                                                            gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                                                                   >  1:107998/1‑150 (MQ=255)                                                            gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                                                                   >  1:766994/1‑150 (MQ=255)                                                                    cACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAttt                                                                           <  2:766552/150‑1 (MQ=255)                                                                      ccAAAACCTTTATCGTCGATTGCCAGGGCAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                                                                         >  2:129050/1‑150 (MQ=255)                                                                       cAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                                                                       >  2:724522/1‑151 (MQ=255)                                                                             cTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagc                                                                 >  2:230751/1‑151 (MQ=255)                                                                                tATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagc                                                              >  2:333747/1‑151 (MQ=255)                                                                                 aTCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                                                             >  1:407177/1‑151 (MQ=255)                                                                                   cgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                                                            <  2:569587/150‑1 (MQ=255)                                                                                       gATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt                                                       <  1:553799/151‑1 (MQ=255)                                                                                             cAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt                                                  >  1:486362/1‑150 (MQ=255)                                                                                              aGGGTAATCTCGAACTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                                                 >  2:252888/1‑150 (MQ=255)                                                                                               gggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaaa                                               <  2:554473/151‑1 (MQ=255)                                                                                                ggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa                                                >  2:415599/1‑149 (MQ=255)                                                                                                ggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAg                                              <  1:708014/151‑1 (MQ=255)                                                                                                   aaTCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAAt                                           >  2:191311/1‑151 (MQ=255)                                                                                                               tAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGcc                               >  1:280611/1‑151 (MQ=255)                                                                                                               tAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGcc                               >  2:755599/1‑151 (MQ=255)                                                                                                                aGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGccc                              >  1:586895/1‑151 (MQ=255)                                                                                                                   cacaGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGc                           <  2:71828/151‑1 (MQ=255)                                                                                                                      aGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCg                         >  1:311074/1‑150 (MQ=255)                                                                                                                       gACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGAc                       <  1:213669/151‑1 (MQ=255)                                                                                                                          tACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGAc                       >  2:348636/1‑148 (MQ=255)                                                                                                                              cATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCa                     >  1:708872/1‑146 (MQ=255)                                                                                                                              cATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCa                     <  2:708829/146‑1 (MQ=255)                                                                                                                                 atCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAg             <  1:264409/151‑1 (MQ=255)                                                                                                                                   cAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGt            <  1:597622/150‑1 (MQ=255)                                                                                                                                      ggTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtt        >  1:130356/1‑151 (MQ=255)                                                                                                                                      ggTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtt        >  1:733092/1‑151 (MQ=255)                                                                                                                                       gTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttt       >  2:363164/1‑151 (MQ=255)                                                                                                                                        ttAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtttt      >  2:374153/1‑151 (MQ=255)                                                                                                                                         tAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttttt     >  2:302980/1‑151 (MQ=255)                                                                                                                                         tAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttttt     >  2:169004/1‑151 (MQ=255)                                                                                                                                           aCTGCGCCAACCAGACTCAGTTCGCCATCGACCAGCACGCTCTTTGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGACAGCAGTTCCGTAAAGAATGCGTGGCTGGCCCAGCCGAGCAGCATTGAGTCAtatt      <  2:642454/148‑1 (MQ=255)                                                                                                                                            cTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTcg  >  2:599981/1‑151 (MQ=255)                                                                                                                                            cTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTcg  >  2:569053/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    AGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCG  >  NC_000913/1307249‑1307538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑