Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,356,140	T→C	N324S (AAC→AGC)	sapA ←	antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,140	0	T	C	100.0%	247.9 / NA	66	N324S (AAC→AGC)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/23);  total (43/23)

CAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGG  >  NC_000913/1355998‑1356287                                                                                                                                               |                                                                                                                                                      cAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATc                                                                                                                                               <  1:757253/151‑1 (MQ=255) cAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATc                                                                                                                                               >  1:493342/1‑151 (MQ=255)  aaCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCg                                                                                                                                              >  2:348335/1‑151 (MQ=255)  aaCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCg                                                                                                                                              >  2:100292/1‑151 (MQ=255)    cGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                            >  1:238515/1‑151 (MQ=255)     gACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                            >  1:198750/1‑150 (MQ=255)      aCTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                            >  1:631870/1‑149 (MQ=255)      aCTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAg                                                                                                                                          <  1:403259/151‑1 (MQ=255)        ttCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTg                                                                                                                                        >  1:452528/1‑151 (MQ=255)         tCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGt                                                                                                                                         <  2:652611/149‑1 (MQ=255)              tGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAgcg                                                                                                                                  >  2:757708/1‑151 (MQ=255)              tGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAgcg                                                                                                                                  >  1:697359/1‑151 (MQ=255)                    cgcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGc                                                                                                                            >  2:125551/1‑151 (MQ=255)                    cgcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGc                                                                                                                            >  1:372039/1‑151 (MQ=255)                     gcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCg                                                                                                                           >  1:257260/1‑151 (MQ=255)                       gATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGa                                                                                                                         <  2:718848/151‑1 (MQ=255)                          ttCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACa                                                                                                                       <  2:119344/150‑1 (MQ=255)                           tCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAg                                                                                                                      <  2:692084/150‑1 (MQ=255)                              ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                                  >  2:579885/1‑151 (MQ=255)                               cGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                                  >  2:45805/1‑150 (MQ=255)                               cGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                                  >  1:119027/1‑150 (MQ=255)                                ggATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGat                                                                                                                >  2:88428/1‑151 (MQ=255)                                   ttGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGattat                                                                                                             >  2:451622/1‑151 (MQ=255)                                       aTTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTAttt                                                                                                           >  1:161277/1‑149 (MQ=255)                                            gTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcggc‑g                                                                                                   <  1:100293/151‑1 (MQ=255)                                            gTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcggc‑g                                                                                                   >  1:425166/1‑151 (MQ=255)                                              aaTTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGt                                                                                                 <  1:244837/151‑1 (MQ=255)                                               aTTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGtt                                                                                                <  1:574814/151‑1 (MQ=255)                                                     gCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGGTTTgggg                                                                                           >  2:724690/1‑149 (MQ=255)                                                         cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGcggc‑gggcgcgcggtggt                                                                                      >  2:360104/1‑139 (MQ=255)                                                           ttGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGGTTGGC‑Ggtggt                                                                                     >  1:301560/1‑147 (MQ=255)                                                            tGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑Ggtggt                                                                                     >  1:159497/1‑146 (MQ=255)                                                              tCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGC‑gggggtta                                                                                   >  1:111923/1‑142 (MQ=255)                                                               cATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑gggggttaa                                                                                  >  2:589222/1‑141 (MQ=255)                                                                      ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGGTATATg                                                                                >  1:9749/1‑143 (MQ=255)                                                                      ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCACCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAg                                                                        <  1:211487/151‑1 (MQ=255)                                                                       cACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAgg                                                                       <  1:403483/151‑1 (MQ=255)                                                                          gAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGTTAAATGCCAGATAgg                                                                       >  1:569853/1‑148 (MQ=255)                                                                                   ggTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATcc                                                           <  1:161757/151‑1 (MQ=255)                                                                                   ggTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGTTTAATGCCAGATAAGCGGCGTTCATcc                                                           >  1:11921/1‑151 (MQ=255)                                                                                            gagGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGCGGGGGTTAAATG‑CAGATAAGCGACGTTTATCCCCGGAAGc                                                   >  2:97431/1‑150 (MQ=255)                                                                                                 cgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGtt                                             <  2:514642/151‑1 (MQ=255)                                                                                                 cgcagTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTTC‑GGTGGTTAATCCCCGAATGGCGACGTTTATCCCCGGAcgccgc                                                >  2:765800/1‑148 (MQ=255)                                                                                                    cgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGG‑GGGGGTAAATGCCCGATAAGGGACGTTCTTCCCAGGACGCAGCggtta                                           >  2:765689/1‑146 (MQ=255)                                                                                                     gTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGC‑GGTGTTTAATGCCAGATAAGCGGCGTTCATCCCCGGACCCAGCGTTaaa                                          >  1:673151/1‑150 (MQ=255)                                                                                                        tCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAAAGCCAAAAAGGCGGAGTTTATCCCCGGGCGCAGCGTTaaa                                          >  1:556053/1‑147 (MQ=255)                                                                                                          aGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGGTAAAAGCCAGAAAGGCGACGTTCCTCCCCGGACGCAGCGTTTAACGcca                                     >  2:517929/1‑148 (MQ=255)                                                                                                           gCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAgc                                   <  2:411251/151‑1 (MQ=255)                                                                                                            ccGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGGTAAAAGGCACATAAGGGACGGTCATCCCAGGACCCAGCGTTtaa                                          >  2:725321/1‑143 (MQ=255)                                                                                                             cGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGTTAAAAGCCAGATAGGCGACGTTCATCCCCGGACGCAGCGGTAAACGCaa                                     >  1:477738/1‑147 (MQ=255)                                                                                                                 ccATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgt                             <  2:198742/151‑1 (MQ=255)                                                                                                                    taataGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcg                           <  2:334513/150‑1 (MQ=255)                                                                                                                         gATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGc                     <  1:552680/151‑1 (MQ=255)                                                                                                                              aTTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGGTTGTC‑GGGGGTAAAAGCCCGAAAAGCGCCGTTCATCCCAAGGCGGAGCGGTGAAAGCAAAAAGGGGTCGTCCCCCaaaa                 >  1:728534/1‑150 (MQ=255)                                                                                                                                tGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATg               <  2:631083/150‑1 (MQ=255)                                                                                                                                  cATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGAt            <  1:37867/151‑1 (MQ=255)                                                                                                                                  cATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGAt            <  1:217702/151‑1 (MQ=255)                                                                                                                                   aTCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGC‑GGTGTTAAATGCCAGAAAGGCGACGTTCATTCCCAGACGCAGGGGTAAACGCAAGCGCGGGGCGTCACGCAAAATgggt            >  1:710329/1‑148 (MQ=255)                                                                                                                                   aTCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATa           <  1:725365/151‑1 (MQ=255)                                                                                                                                   aTCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGATTTGC‑GGGGGTAAAAGTCAGATAAGCGGCGTTCATCCCCGGACGCAGCGTTAAAAGCAAGCGCGGGGCGTCACGCaaaa                 >  1:507027/1‑145 (MQ=255)                                                                                                                                       ggCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGC‑GGGGGTAAATACCAAACTTGCGACGTTCAATCCCGAACGCCGCGTTAAAAGAAAGTGCCGGGCGTCTCGCaaaaaaccaga          >  2:395509/1‑141 (MQ=255)                                                                                                                                       ggCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCaaa                  <  1:395530/140‑1 (MQ=255)                                                                                                                                        gcgcTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctgg      <  2:108172/151‑1 (MQ=255)                                                                                                                                          gcTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGG‑GGGGTTAAAAGCCAGAAAAGCGACGGTCATCCCCGGACCCAGCGGTTAAAGCAAACGCGGGTCGTCACGCAAAAAGGGTAgctgg      >  2:99360/1‑149 (MQ=255)                                                                                                                                           cTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGG‑GGTGGTAAAAAGCAAATAGGAGGGGTTCTTCCCCAGACGCCGGGTTTAACGCAAGGGCGGGGTGGCCCGCCAAATGGAAAggtggct    >  1:687282/1‑150 (MQ=255)                                                                                                                                            tGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGGGTTAAATGCCAGAAAGGCGGCGGTCATCCCAGGACGCAGCGGTAAAAGCAAGCGCGGGTGGTCACGCAAAATGGGTAGctggctgg  >  1:619137/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                      CAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTGGC‑GGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGG  >  NC_000913/1355998‑1356287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑