Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,721	A→G	F351F (TTT→TTC)	abgT ←	p‑aminobenzoyl‑glutamate transporter; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,721	0	A	G	100.0%	158.9 / NA	43	F351F (TTT→TTC)	abgT	p‑aminobenzoyl‑glutamate transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/29);  total (14/29)

ATGCATAAGAAAGAGGAAAGCAACGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGAAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAC  >  NC_000913/1400575‑1400866                                                                                                                                                   |                                                                                                                                                  aTGCATAAGAAAGAGGAAAGCAACGCCAGACCGACAAACACCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGgaaaa                                                                                                                                               <  2:143939/151‑1 (MQ=255)       aagaaagaGGAAAGCAACGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATc                                                                                                                                          <  1:129148/150‑1 (MQ=255)                  aaGCAACGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATccc                                                                                                                              <  1:51024/151‑1 (MQ=255)                   aGCAACGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCg                                                                                                                             >  1:697899/1‑151 (MQ=255)                        cGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCAtc                                                                                                                        >  2:400282/1‑151 (MQ=255)                        cGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCAtc                                                                                                                        <  1:319905/151‑1 (MQ=255)                         gCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCAtct                                                                                                                       <  2:532557/151‑1 (MQ=255)                          ccAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCAtctc                                                                                                                      <  2:150172/151‑1 (MQ=255)                             gACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCttt                                                                                                                   <  2:335593/151‑1 (MQ=255)                                cGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCa                                                                                                                 <  2:493661/150‑1 (MQ=255)                                 gACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATc                                                                                                               <  2:274444/151‑1 (MQ=255)                                           gggATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATc                                                                                                      >  2:408839/1‑150 (MQ=255)                                                          ccTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAAt                                                                                      >  2:91969/1‑151 (MQ=255)                                                               aCTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGcc                                                                                 <  2:705895/151‑1 (MQ=255)                                                                  ttCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTg                                                                               <  2:558994/150‑1 (MQ=255)                                                                   tCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGa                                                                              <  1:759600/150‑1 (MQ=255)                                                                         atatCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGaa                                                                       <  1:180053/151‑1 (MQ=255)                                                                         atatCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGa                                                                        <  1:190425/150‑1 (MQ=255)                                                                         atatCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGa                                                                        <  1:672093/150‑1 (MQ=255)                                                                                  aGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCggg                                                              >  1:732576/1‑151 (MQ=255)                                                                                            cGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGcc                                                    <  1:400303/151‑1 (MQ=255)                                                                                               tGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCAt                                                  >  2:18793/1‑150 (MQ=255)                                                                                               tGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCAt                                                  >  2:389351/1‑150 (MQ=255)                                                                                                  aTTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGc                                              <  1:262391/151‑1 (MQ=255)                                                                                                  aTTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGc                                              <  1:26761/151‑1 (MQ=255)                                                                                                   tttCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGcc                                             >  1:447867/1‑151 (MQ=255)                                                                                                      ccccATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAg                                           <  1:504914/150‑1 (MQ=255)                                                                                                      ccccATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGc                                          <  1:75737/151‑1 (MQ=255)                                                                                                      ccccATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGc                                          >  1:245245/1‑151 (MQ=255)                                                                                                      ccccATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGc                                          <  2:496947/151‑1 (MQ=255)                                                                                                               gCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGacaaca                                 <  2:300904/151‑1 (MQ=255)                                                                                                                  ccAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGAcaacaa                                <  2:245235/149‑1 (MQ=255)                                                                                                                  ccAGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACaaa                               <  1:34783/150‑1 (MQ=255)                                                                                                                    aGTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGa                             <  2:27593/150‑1 (MQ=255)                                                                                                                     gTTAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGaaa                           <  2:411038/151‑1 (MQ=255)                                                                                                                      ttAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGaaa                           >  1:51946/1‑150 (MQ=255)                                                                                                                       tAAACATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGaaaa                          <  2:241305/150‑1 (MQ=255)                                                                                                                           cATGGCGACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGa                     <  2:12075/151‑1 (MQ=255)                                                                                                                                 gACAAATTGGGCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATc               >  1:440000/1‑151 (MQ=255)                                                                                                                                         gggCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCAc       >  2:245455/1‑151 (MQ=255)                                                                                                                                         gggCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCAc       <  1:28307/151‑1 (MQ=255)                                                                                                                                          ggCGAGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACg      >  1:631018/1‑151 (MQ=255)                                                                                                                                              aGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAc  >  2:163655/1‑151 (MQ=255)                                                                                                                                              aGGGGGAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAc  <  1:163662/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATGCATAAGAAAGAGGAAAGCAACGCCAGACCGACAAACGCCGGGATGCCGCTAAGCCCTGAACTTTCCAGTATATCGGTCAGCCCCACGGCGATGAATTTCCCCATGTTGCTCCAGTTAAACATGGCGACAAATTGGGCGAGGGGAAAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAC  >  NC_000913/1400575‑1400866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑