Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,951	C→T	intergenic (+302/‑28)	abgR → / → smrA	putative DNA‑binding transcriptional regulator of abgABT operon/DNA endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,951	0	C	T	100.0%	139.2 / NA	35	intergenic (+302/‑28)	abgR/smrA	putative DNA‑binding transcriptional regulator of abgABT operon/DNA endonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/5);  total (30/5)

AAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATC  >  NC_000913/1405813‑1406095                                                                                                                                           |                                                                                                                                                 aaaTATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAAtt                                                                                                                                      <  2:445282/151‑1 (MQ=255)                tattaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAAc                                                                                                                       >  2:327905/1‑151 (MQ=255)                 attaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAAc                                                                                                                       >  2:670506/1‑150 (MQ=255)                  ttaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACAt                                                                                                                     >  1:448323/1‑151 (MQ=255)                        tCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCt                                                                                                               >  1:191181/1‑151 (MQ=255)                          gTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTg                                                                                                             >  2:138782/1‑151 (MQ=255)                           tgaatgaaTAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTg                                                                                                             >  1:28252/1‑150 (MQ=255)                                      tCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGc                                                                                                 >  1:168080/1‑151 (MQ=255)                                      tCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGc                                                                                                 >  2:122666/1‑151 (MQ=255)                                           cATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGttt                                                                                            >  2:755146/1‑151 (MQ=255)                                           cATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGttt                                                                                            >  1:404345/1‑151 (MQ=255)                                             tAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                          >  1:349822/1‑151 (MQ=255)                                              aaGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                          <  1:68722/150‑1 (MQ=255)                                                  aTTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACg                                                                                     >  1:259150/1‑151 (MQ=255)                                                                   ggCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTcc                                                                      >  1:687148/1‑149 (MQ=255)                                                                    gCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGc                                                                   >  1:563566/1‑151 (MQ=255)                                                                     cAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGcc                                                                  >  2:354549/1‑151 (MQ=255)                                                                               aaCTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGt                                                        >  2:328512/1‑151 (MQ=255)                                                                                     ttaCTGGCAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTAcc                                                  >  1:418313/1‑151 (MQ=255)                                                                                      taCTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCg                                                 >  1:480927/1‑151 (MQ=255)                                                                                             aaTTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTcc                                           >  1:583254/1‑150 (MQ=255)                                                                                              aTTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCAc                                         >  2:133631/1‑151 (MQ=255)                                                                                               tttGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACt                                        >  1:216428/1‑151 (MQ=255)                                                                                                 tGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTg                                       >  2:77647/1‑150 (MQ=255)                                                                                                    gTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCAt                                   >  1:398969/1‑151 (MQ=255)                                                                                                       aTACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATcc                                 >  1:373620/1‑150 (MQ=255)                                                                                                          cGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAAcg                             >  1:180941/1‑151 (MQ=255)                                                                                                             tgttgGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATCGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGt                          >  2:1664/1‑151 (MQ=255)                                                                                                                          cGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTgcgc             <  1:327918/151‑1 (MQ=255)                                                                                                                             tCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCTCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGcgccgc          >  2:300738/1‑151 (MQ=255)                                                                                                                                ggTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCTCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGc       <  1:300751/151‑1 (MQ=255)                                                                                                                                ggTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGc       >  2:455627/1‑151 (MQ=255)                                                                                                                                    aTCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTAt   >  1:145571/1‑151 (MQ=255)                                                                                                                                    aTCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTAt   >  2:575242/1‑151 (MQ=255)                                                                                                                                      cATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATc  <  1:670546/150‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                 AAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATC  >  NC_000913/1405813‑1406095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑