Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,514,041	A→G	T71A (ACG→GCG)	ydcU →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,514,041	0	A	G	100.0%	201.5 / NA	58	T71A (ACG→GCG)	ydcU	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

GGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCGGGAAA  >  NC_000913/1513895‑1514187                                                                                                                                                   |                                                                                                                                                   ggCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCg                                                                                                                                                  <  1:172675/149‑1 (MQ=255)  gCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTGACGATTTCACCATGTCGGGAACGCCGGAACTGGCGCTg                                                                                                                                               >  1:412925/1‑151 (MQ=255)       aTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGa                                                                                                                                           >  1:158936/1‑150 (MQ=255)                tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGCTTTTTTACTTTTGACGGTTTTCCCCTTTCGGTAACGCCGGAAGTGGCGCTGGCGGATATCCGt                                                                                                                                   >  2:48376/1‑149 (MQ=255)                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCt                                                                                                                              >  2:670790/1‑149 (MQ=255)                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGATGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCt                                                                                                                              <  2:366156/149‑1 (MQ=255)                        tttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGttt                                                                                                                          >  1:39664/1‑150 (MQ=255)                          tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTaa                                                                                                                        <  1:225420/150‑1 (MQ=255)                             aCTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTAGTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGATTTATAATTTTTACGATTTCACCATGTCTGTAACGCCGGAAAATGGGCTGGGCGATAGCCGTGCGCAGTTTaa                                                                                                                        >  2:556171/1‑147 (MQ=255)                                   gctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGa                                                                                                                <  1:170457/149‑1 (MQ=255)                                   gctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAt                                                                                                              <  1:430454/151‑1 (MQ=255)                                   gctTGGCCCGCTAAATGGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATTCTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAt                                                                                                              <  2:141096/151‑1 (MQ=255)                                     ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAtt                                                                                                             >  1:399460/1‑150 (MQ=255)                                           cGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcat                                                                                                      <  1:207903/151‑1 (MQ=255)                                             cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatc                                                                                                     >  1:417764/1‑150 (MQ=255)                                                 tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGAcatcct                                                                                                   >  2:389305/1‑146 (MQ=255)                                                  gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                                                                <  1:673367/150‑1 (MQ=255)                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGATGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                                                              >  2:177435/1‑150 (MQ=255)                                                        tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAAAGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                                                                          >  2:643343/1‑150 (MQ=255)                                                        tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTGCGCAc                                                                                          <  1:607403/150‑1 (MQ=255)                                                            aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGa                                                                                     <  2:131871/151‑1 (MQ=255)                                                               gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                                                                <  1:315472/137‑1 (MQ=255)                                                               gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                                                                >  2:315459/1‑137 (MQ=255)                                                               gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACt                                                                                   <  2:210348/150‑1 (MQ=255)                                                                 cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTAt                                                                                 >  1:72753/1‑150 (MQ=255)                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATgg                                                                               <  2:96695/150‑1 (MQ=255)                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGc                                                                              <  1:496334/151‑1 (MQ=255)                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAATACGACATCATTCTCCGCACGCTGACTTTgg                                                                               >  2:19300/1‑150 (MQ=255)                                                                    tttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCCTGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCt                                                                             >  1:392777/1‑151 (MQ=255)                                                                        ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTgg                                                                         <  1:515414/151‑1 (MQ=255)                                                                         gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGc                                                                        <  1:566857/151‑1 (MQ=255)                                                                             gctgctGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTc                                                                    >  1:435138/1‑151 (MQ=255)                                                                                gctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCAc                                                                  <  1:223791/150‑1 (MQ=255)                                                                                gctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCAc                                                                  >  1:5757/1‑150 (MQ=255)                                                                                   gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                                                               <  2:40500/150‑1 (MQ=255)                                                                                         gTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCa                                                          <  2:321107/149‑1 (MQ=255)                                                                                              ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCa                                                    <  2:51050/150‑1 (MQ=255)                                                                                               gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt                                                  <  1:310119/151‑1 (MQ=255)                                                                                                    gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGc                                             >  1:122425/1‑151 (MQ=255)                                                                                                       tttATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCtt                                           <  2:631417/150‑1 (MQ=255)                                                                                                          ataCTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTcc                                       >  2:669385/1‑151 (MQ=255)                                                                                                           taCTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTcc                                       >  2:598276/1‑150 (MQ=255)                                                                                                            aCTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCa                                      >  1:533309/1‑150 (MQ=255)                                                                                                                ttGACGATTTCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATgg                                  <  1:301417/150‑1 (MQ=255)                                                                                                                        ttCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGtat                          <  2:656445/150‑1 (MQ=255)                                                                                                                         tCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTatat                        >  1:46514/1‑151 (MQ=255)                                                                                                                         tCACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTatat                        >  1:371293/1‑151 (MQ=255)                                                                                                                          cACCATGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTatat                        <  2:660319/150‑1 (MQ=255)                                                                                                                               tGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGgcgcg                  >  2:40445/1‑151 (MQ=255)                                                                                                                               tGTCGGTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGgcgc                   <  2:287641/150‑1 (MQ=255)                                                                                                                                    gTAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCtata             <  1:375698/151‑1 (MQ=255)                                                                                                                                     tAACGCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATAc            >  2:300693/1‑151 (MQ=255)                                                                                                                                         gCCGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGc        <  2:254822/151‑1 (MQ=255)                                                                                                                                           cGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCgg      <  1:605885/151‑1 (MQ=255)                                                                                                                                           cGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCgg      <  2:634586/151‑1 (MQ=255)                                                                                                                                           cGGAACTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCgg      <  1:55085/151‑1 (MQ=255)                                                                                                                                               aCTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCGGGaaa  <  1:643382/151‑1 (MQ=255)                                                                                                                                               aCTGGCGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGGATATGGCGCGCTATACCAGCGGGa    <  2:670679/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGCTTTTCCAATGGCGTGGTATATGGCGCGCTATACCAGCGGGAAA  >  NC_000913/1513895‑1514187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑