Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,775	G→A	G455S (GGT→AGT)	ftsI →	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,775	0	G	A	100.0%	146.0 / NA	38	G455S (GGT→AGT)	ftsI	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

GTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCG  >  NC_000913/92633‑92921                                                                                                                                               |                                                                                                                                                   gtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACgt                                                                                                                                            <  2:459362/151‑1 (MQ=255)   cTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACgtgt                                                                                                                                          >  1:144382/1‑151 (MQ=255)         tAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTccc                                                                                                                                    >  1:549796/1‑151 (MQ=255)                        tctcTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCg                                                                                                                     >  2:467113/1‑151 (MQ=255)                            tttCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGCCGCTCACAAAAGTTGACCACGCGGTTCACAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCAcc                                                                                                                 >  2:623883/1‑150 (MQ=255)                                    aCGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGc                                                                                                          >  1:202170/1‑150 (MQ=255)                                               gTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGaaa                                                                                              <  2:12681/151‑1 (MQ=255)                                                          aCAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGTCCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATCATGGAAATCGTGGCGCt                                                                                    <  2:597194/150‑1 (MQ=255)                                                                     gAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggcggcgg                                                                        >  1:525984/1‑151 (MQ=255)                                                                       gTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggcggcggcg                                                                      <  1:486179/151‑1 (MQ=255)                                                                           aCGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggcggcggcggc                                                                    <  2:544813/149‑1 (MQ=255)                                                                            cGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTg                                                                 <  1:221287/151‑1 (MQ=255)                                                                                  tATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCatat                                                                                                      <  2:345393/108‑1 (MQ=255)                                                                                  tATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCatat                                                                                                      >  1:345407/1‑108 (MQ=255)                                                                                  tATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAggcg                                                           >  2:751063/1‑151 (MQ=255)                                                                                   aTCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAggcg                                                           <  2:186785/150‑1 (MQ=255)                                                                                     cGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGgcggcg                                                        <  2:603792/151‑1 (MQ=255)                                                                                      ggCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGa                                                       <  1:584248/151‑1 (MQ=255)                                                                                             aCGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAg                                                 <  2:523282/150‑1 (MQ=255)                                                                                               ggCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCt                                              <  1:607543/151‑1 (MQ=255)                                                                                               ggCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCt                                              <  2:17596/151‑1 (MQ=255)                                                                                                        cGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg                                     <  1:312901/151‑1 (MQ=255)                                                                                                               tGTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTa                               >  2:455555/1‑150 (MQ=255)                                                                                                                gTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaa                             <  1:477059/151‑1 (MQ=255)                                                                                                                gTCGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaa                              <  1:363676/150‑1 (MQ=255)                                                                                                                  cGATTACCAAAGTTGACCCCCCGGTTCCCATTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAGGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa                            <  2:683961/150‑1 (MQ=255)                                                                                                                  cGATTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAc                           <  1:178417/151‑1 (MQ=255)                                                                                                                    aTTACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCg                         >  1:722357/1‑151 (MQ=255)                                                                                                                      tACCAAAGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGt                       >  2:614648/1‑151 (MQ=255)                                                                                                                           aaGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACcgc                  <  1:238800/151‑1 (MQ=255)                                                                                                                           aaGTTGACCCCCCGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACcgc                  <  2:747147/151‑1 (MQ=255)                                                                                                                                     cccGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTc        >  1:226741/1‑151 (MQ=255)                                                                                                                                      ccGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCg       >  1:574607/1‑151 (MQ=255)                                                                                                                                       cGGTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCgg      <  2:226732/151‑1 (MQ=255)                                                                                                                                        ggTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCggg     >  1:628514/1‑151 (MQ=255)                                                                                                                                        ggTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCggg     <  1:349248/151‑1 (MQ=255)                                                                                                                                         gTTCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGc    >  2:752956/1‑151 (MQ=255)                                                                                                                                           tCCCAGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCg  >  2:203707/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                   GTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCG  >  NC_000913/92633‑92921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑