Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,554	C→T	E251K (GAA→AAA)	ydfU ←	Qin prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,554	0	C	T	100.0%	262.9 / NA	65	E251K (GAA→AAA)	ydfU	Qin prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/29);  total (36/29)

CCATTCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCG  >  NC_000913/1643413‑1643699                                                                                                                                              |                                                                                                                                                  ccATTCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGActc                                                                                                                                          <  2:81483/151‑1 (MQ=255)   aTTCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTg                                                                                                                                        >  1:417022/1‑151 (MQ=255)    ttCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTgg                                                                                                                                       >  2:715738/1‑151 (MQ=255)     tCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTgg                                                                                                                                       >  1:160041/1‑150 (MQ=255)       cGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATc                                                                                                                                    >  1:465138/1‑151 (MQ=255)        gCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATc                                                                                                                                    >  2:405990/1‑150 (MQ=255)          ccTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAAc                                                                                                                                 >  1:618469/1‑151 (MQ=255)          ccTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAAc                                                                                                                                 <  1:690853/151‑1 (MQ=255)                cGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGAt                                                                                                                           <  1:60258/151‑1 (MQ=255)                   gACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCt                                                                                                                        >  2:741864/1‑151 (MQ=255)                     ccAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCtt                                                                                                                       >  1:147976/1‑150 (MQ=255)                      cAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTac                                                                                                                     >  1:401972/1‑151 (MQ=255)                          cAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTaccac                                                                                                                  <  2:617960/150‑1 (MQ=255)                                  gCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCtttt                                                                                                          >  1:126429/1‑150 (MQ=255)                                      aTCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCtgt                                                                                                     <  2:755964/151‑1 (MQ=255)                                       tcgtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCtgtg                                                                                                    >  2:624521/1‑151 (MQ=255)                                       tcgtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCtgtg                                                                                                    >  1:514778/1‑151 (MQ=255)                                           cgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTgtggtg                                                                                                 <  2:313275/150‑1 (MQ=255)                                            ggCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGAt                                                                                               >  2:597796/1‑151 (MQ=255)                                                 gCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTttg                                                                                          <  2:626392/151‑1 (MQ=255)                                                 gCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTttg                                                                                          <  2:546817/151‑1 (MQ=255)                                                       cGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGGTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCtg                                                                                    <  2:188182/151‑1 (MQ=255)                                                        gcagcaAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCtgg                                                                                   <  2:552684/151‑1 (MQ=255)                                                          agcaagcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCtggtg                                                                                 >  2:154495/1‑151 (MQ=255)                                                          agcaagcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCtggtg                                                                                 >  2:241115/1‑151 (MQ=255)                                                            caagcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTggg                                                                               >  2:590914/1‑151 (MQ=255)                                                              agcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCg                                                                             <  2:180270/151‑1 (MQ=255)                                                              agcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCg                                                                             <  2:510417/151‑1 (MQ=255)                                                                cacaCGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGt                                                                            >  1:378336/1‑150 (MQ=255)                                                                  cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTa                                                                           >  1:444623/1‑149 (MQ=255)                                                                  cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTa                                                                           >  1:606392/1‑149 (MQ=255)                                                                  cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTa                                                                           >  2:443396/1‑149 (MQ=255)                                                                  cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTa                                                                           >  1:26372/1‑149 (MQ=255)                                                                   acGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAgg                                                                        <  1:175997/151‑1 (MQ=255)                                                                    cGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGc                                                                       >  1:665605/1‑151 (MQ=255)                                                                     ggCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCa                                                                      <  1:346094/151‑1 (MQ=255)                                                                     ggCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCa                                                                      <  2:253256/151‑1 (MQ=255)                                                                         gtgtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAAc                                                                    <  1:24284/149‑1 (MQ=255)                                                                           gtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGt                                                                 <  1:273691/150‑1 (MQ=255)                                                                           gtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGt                                                                 <  1:234652/150‑1 (MQ=255)                                                                                 aCCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGAt                                                          <  2:241977/151‑1 (MQ=255)                                                                                     caCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGAttttt                                                      >  2:459857/2‑151 (MQ=255)                                                                                      aCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGAtttttt                                                     >  1:48031/1‑151 (MQ=255)                                                                                       cGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTtg                                                    >  1:639850/1‑151 (MQ=255)                                                                                        gtgtgtATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTtgt                                                   <  1:405640/151‑1 (MQ=255)                                                                                        gtgtgtATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTtgt                                                   <  1:339931/151‑1 (MQ=255)                                                                                           tgtATCTCTCCTTAACCCAGCGGCGACGATTAGACCGCTTCATGAACGATTTCGGAGAATCTGGACCAACGGTGATGATTACCACCGCCTTTTTCCGCGGTGCTTTATGCTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTgcgc                                                 >  2:93874/1‑150 (MQ=255)                                                                                            gtATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGATTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCgt                                               >  1:748305/1‑151 (MQ=255)                                                                                            gtATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCgt                                               <  2:444602/151‑1 (MQ=255)                                                                                               tttCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCgttgt                                            >  1:510030/1‑151 (MQ=255)                                                                                                 tctcCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCgttgtt                                           <  2:615836/150‑1 (MQ=255)                                                                                                   tcCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTc                                         >  2:479028/1‑150 (MQ=255)                                                                                                       tAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAata                                     <  1:294275/150‑1 (MQ=255)                                                                                                        aaCCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAatat                                    <  1:147438/150‑1 (MQ=255)                                                                                                         aCCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGc                                  >  1:736599/1‑151 (MQ=255)                                                                                                               cggcgACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCtggtgg                            <  1:267017/151‑1 (MQ=255)                                                                                                                     cGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTc                       >  1:616275/1‑150 (MQ=255)                                                                                                                     cGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTc                       >  1:204663/1‑150 (MQ=255)                                                                                                                       tttAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGc                    >  1:372469/1‑151 (MQ=255)                                                                                                                        ttAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGc                    <  2:642071/150‑1 (MQ=255)                                                                                                                                       aaaGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGt    <  1:174931/151‑1 (MQ=255)                                                                                                                                        aaGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTc   <  1:9355/151‑1 (MQ=255)                                                                                                                                        aaGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTc   >  2:536619/1‑151 (MQ=255)                                                                                                                                         aGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTc   >  2:304612/1‑150 (MQ=255)                                                                                                                                         aGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCg  >  1:395030/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                  CCATTCCGCCCTGCCCGTGACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCG  >  NC_000913/1643413‑1643699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑