Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,696,856	T→C	D66G (GAC→GGC)	uidR ←	transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,696,856	0	T	C	100.0%	123.7 / NA	34	D66G (GAC→GGC)	uidR	transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

ACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCA  >  NC_000913/1696711‑1696999                                                                                                                                                  |                                                                                                                                                aCGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGt                                                                                                                                            <  2:409534/151‑1 (MQ=255)     aTCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAg                                                                                                                                         <  1:445624/150‑1 (MQ=255)        ccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGAt                                                                                                                                       >  2:145256/1‑149 (MQ=255)         cTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGAt                                                                                                                                       <  1:644898/148‑1 (MQ=255)         cTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATa                                                                                                                                      >  2:660507/1‑149 (MQ=255)                      ttCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCa                                                                                                                         >  2:20592/1‑149 (MQ=255)                       tCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAg                                                                                                                       <  2:162100/150‑1 (MQ=255)                         aaCCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGct                                                                                                                    <  2:119643/151‑1 (MQ=255)                            caccaGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGcttct                                                                                                                 <  1:219753/151‑1 (MQ=255)                                       gTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGa                                                                                                       >  2:358312/1‑150 (MQ=255)                                           tCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATgg                                                                                                  >  2:357939/1‑151 (MQ=255)                                                 ggCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGAt                                                                                              >  1:529002/1‑149 (MQ=255)                                                          ggTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCg                                                                                   <  1:135771/151‑1 (MQ=255)                                                            tGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCggg                                                                                 >  1:713149/1‑151 (MQ=255)                                                              agagagaCAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGAc                                                                               <  1:29832/151‑1 (MQ=255)                                                                agagaCAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt                                                                              >  2:463023/1‑150 (MQ=255)                                                                      aaTGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGgcg                                                                       >  2:288097/1‑151 (MQ=255)                                                                       aTGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGgcgc                                                                      <  2:622869/151‑1 (MQ=255)                                                                                                aaTGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCg                                              >  2:710332/1‑150 (MQ=255)                                                                                                  tGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAgg                                           >  1:766665/1‑151 (MQ=255)                                                                                                    aaTCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCa                                         <  1:584468/151‑1 (MQ=255)                                                                                                           tCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACtgtg                                    <  1:550232/149‑1 (MQ=255)                                                                                                            cAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGaa                                  >  1:293071/1‑150 (MQ=255)                                                                                                                  gTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCAtt                           <  2:723117/151‑1 (MQ=255)                                                                                                                  gTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCAtt                           <  2:214890/151‑1 (MQ=255)                                                                                                                    tCCCGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCAttt                          <  1:22641/150‑1 (MQ=255)                                                                                                                      ccGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCt                       >  2:313244/1‑151 (MQ=255)                                                                                                                       cGGAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTg                      <  2:428887/151‑1 (MQ=255)                                                                                                                        ggAAACGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTg                      >  1:377298/1‑150 (MQ=255)                                                                                                                            aCGGGCCAGCGCCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGaaaa                  >  1:206730/1‑150 (MQ=255)                                                                                                                                     cgcCCTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTg        <  2:713106/151‑1 (MQ=255)                                                                                                                                        ccTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgca     <  1:75236/151‑1 (MQ=255)                                                                                                                                        ccTCTCCTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgca     <  1:288109/151‑1 (MQ=255)                                                                                                                                             ccTGGCCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgcagca  <  1:358327/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                ACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCA  >  NC_000913/1696711‑1696999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑