Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,768,945	A→G	intergenic (‑260/‑129)	ydiJ ← / → ydiK	putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,768,945	0	A	G	100.0%	180.1 / NA	51	intergenic (‑260/‑129)	ydiJ/ydiK	putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

GCGGCAAGCGAGTGAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGC  >  NC_000913/1768804‑1769092                                                                                                                                              |                                                                                                                                                    gcggcAAGCGAGTGAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCa                                                                                                                                            <  2:761724/151‑1 (MQ=255)     cAAGCGAGTGAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAAt                                                                                                                                        <  1:477646/151‑1 (MQ=255)           aGTGAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAggg                                                                                                                                  <  1:449196/151‑1 (MQ=255)              gAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCa                                                                                                                                <  2:595904/150‑1 (MQ=255)               aaCTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGa                                                                                                                              <  1:458416/151‑1 (MQ=255)                     aGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGc                                                                                                                        <  2:318887/151‑1 (MQ=255)                       gAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCt                                                                                                                      <  2:359679/151‑1 (MQ=255)                        aGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTg                                                                                                                     <  2:15053/151‑1 (MQ=255)                            tACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTa                                                                                                                  >  1:581307/1‑150 (MQ=255)                               aaTAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTaaaaa                                                                                                              <  1:22452/151‑1 (MQ=255)                               aaTAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTaaaa                                                                                                               >  1:625474/1‑150 (MQ=255)                                 tAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAAcc                                                                                                            >  2:611768/1‑151 (MQ=255)                                 tAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAAcc                                                                                                            >  2:521281/1‑151 (MQ=255)                                  aGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAAcc                                                                                                            <  2:591361/150‑1 (MQ=255)                                                   aacgaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTcatcat                                                                                          <  1:702962/151‑1 (MQ=255)                                                     cgaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCAtt                                                                                         >  1:139429/1‑150 (MQ=255)                                                      gaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTa                                                                                        >  1:629554/1‑150 (MQ=255)                                                      gaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTAt                                                                                       >  1:153700/1‑151 (MQ=255)                                                           tAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAgg                                                                                  >  2:695075/1‑151 (MQ=255)                                                               cGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTg                                                                                >  1:405789/1‑149 (MQ=255)                                                               cGCAGAACAGGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGGCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCGATCAGAATTGAGGGCAGAATCGGACTGTTAAAAACCGCGGGAATTACTCTTCATTATGCAGGTg                                                                                >  2:335923/1‑149 (MQ=255)                                                                  agcaCATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGttt                                                                           <  1:705580/151‑1 (MQ=255)                                                                           aaCTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGGGtt                                                                   >  2:445623/1‑150 (MQ=255)                                                                               tGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgt                                                              >  1:136844/1‑151 (MQ=255)                                                                                gAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtc                                                             >  2:675598/1‑151 (MQ=255)                                                                                gAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtc                                                             <  1:367986/151‑1 (MQ=255)                                                                                    tACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgt                                                         >  1:90600/1‑151 (MQ=255)                                                                                        acgaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgac                                                     >  1:651511/1‑151 (MQ=255)                                                                                         cgaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgacg                                                    <  1:569219/151‑1 (MQ=255)                                                                                          gaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgacga                                                   <  2:578408/151‑1 (MQ=255)                                                                                          gaGGAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgac                                                     <  1:497534/149‑1 (MQ=255)                                                                                           aGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt                                                  <  2:347231/151‑1 (MQ=255)                                                                                                  cGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACg                                            <  1:55771/150‑1 (MQ=255)                                                                                                  cGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGc                                           >  2:642853/1‑151 (MQ=255)                                                                                                  cGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGc                                           >  2:51771/1‑151 (MQ=255)                                                                                                   gTTTGCGTGTTGCCTGAGTTGCTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGc                                           >  2:646113/1‑150 (MQ=255)                                                                                                    tttGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGcac                                         >  2:214848/1‑151 (MQ=255)                                                                                                         gtgtTGCCTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaaa                                    <  2:176856/151‑1 (MQ=255)                                                                                                                cTGAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGa                              >  1:629404/1‑150 (MQ=255)                                                                                                                  gAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGaaa                            >  2:143026/1‑150 (MQ=255)                                                                                                                  gAGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGaaa                            <  1:141210/150‑1 (MQ=255)                                                                                                                   aGTTGTTGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAag                          <  2:481754/151‑1 (MQ=255)                                                                                                                    gttgttGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAagt                         <  1:369765/151‑1 (MQ=255)                                                                                                                    gttgttGTACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAag                          <  2:128152/150‑1 (MQ=255)                                                                                                                      tgttgtACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAagta                        <  1:558776/150‑1 (MQ=255)                                                                                                                       gttgtACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAagtagt                      >  2:578115/1‑151 (MQ=255)                                                                                                                          gtACCACATTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTa                    <  1:6934/150‑1 (MQ=255)                                                                                                                                caTTTTTTTCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTaa              >  1:244858/1‑150 (MQ=255)                                                                                                                                       ttCTAACGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGt      >  1:60805/1‑151 (MQ=255)                                                                                                                                           aaCGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCa    <  1:675639/149‑1 (MQ=255)                                                                                                                                           aaCGCGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGc  >  1:643068/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    GCGGCAAGCGAGTGAACTCCAGGAGCTTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGC  >  NC_000913/1768804‑1769092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑