Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,787,381 A→G intergenic (‑269/‑64) ppsA ← / → ppsR phosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,787,3810AG100.0% 34.3 / NA 10intergenic (‑269/‑64)ppsA/ppsRphosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/1);  total (9/1)

TAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATT  >  NC_000913/1787235‑1787512
                                                                                                                                                  |                                                                                                                                   
tAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCt                                                                                                                                 >  2:150463/1‑151 (MQ=255)
                                          aTTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGc                                                                                       >  2:15846/1‑151 (MQ=255)
                                                                                            ttttCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTtat                                     >  2:201750/1‑151 (MQ=255)
                                                                                                 aTAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTc                                >  2:462498/1‑151 (MQ=255)
                                                                                                      aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATg                           >  2:519444/1‑151 (MQ=255)
                                                                                                          aaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTAc                       >  2:489906/1‑151 (MQ=255)
                                                                                                           aaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACg                      >  2:390916/1‑151 (MQ=255)
                                                                                                                    gTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg                                             >  1:559651/1‑119 (MQ=255)
                                                                                                                    gTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg                                             <  2:559623/119‑1 (MQ=255)
                                                                                                                               aaaCCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTAtt  >  1:288546/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                   
TAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATT  >  NC_000913/1787235‑1787512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: