Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,808,794	T→C	H147R (CAC→CGC)	yniB ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,808,794	0	T	C	100.0%	167.0 / NA	45	H147R (CAC→CGC)	yniB	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

AGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCA  >  NC_000913/1808647‑1808938                                                                                                                                                    |                                                                                                                                                 agcagcATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGgcgc                                                                                                                                                <  1:694772/150‑1 (MQ=255)         gATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAacaaca                                                                                                                                       <  1:414401/151‑1 (MQ=255)            atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAAt                                                                                                                                     <  1:319439/150‑1 (MQ=255)            atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAAt                                                                                                                                     <  1:332891/150‑1 (MQ=255)            atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATa                                                                                                                                    <  2:636684/151‑1 (MQ=255)               ggTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACgg                                                                                                                                 >  2:188860/1‑151 (MQ=255)                 ttGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACggg                                                                                                                                >  2:466793/1‑150 (MQ=255)                   gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGAGGAACAACAATACGGGACt                                                                                                                             <  2:686582/151‑1 (MQ=255)                    aGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTc                                                                                                                            >  2:765163/1‑151 (MQ=255)                       aTAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAAt                                                                                                                         <  1:653138/151‑1 (MQ=255)                               cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCAc                                                                                                                 >  2:224226/1‑151 (MQ=255)                                  tCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCAcgcg                                                                                                              >  1:429866/1‑151 (MQ=255)                                   ccGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGt                                                                                                             <  2:32330/151‑1 (MQ=255)                                     gTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTa                                                                                                           <  2:260885/151‑1 (MQ=255)                                           ccTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCct                                                                                                     <  2:290310/151‑1 (MQ=255)                                                   ttAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCt                                                                                             >  1:118561/1‑151 (MQ=255)                                                     aaataaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCtt                                                                                            <  2:29184/150‑1 (MQ=255)                                                              cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTc                                                                                  >  1:201692/1‑151 (MQ=255)                                                                 gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTcc                                                                                 >  1:598824/1‑149 (MQ=255)                                                                   agagAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGa                                                                              >  1:248175/1‑150 (MQ=255)                                                                    gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAAt                                                                            <  1:145658/151‑1 (MQ=255)                                                                        aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGt                                                                        <  1:612843/151‑1 (MQ=255)                                                                        aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGt                                                                        <  1:604039/151‑1 (MQ=255)                                                                           gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGTCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGa                                                                     <  2:274522/151‑1 (MQ=255)                                                                            aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGa                                                                     <  2:364611/150‑1 (MQ=255)                                                                               acaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCtt                                                                 >  1:463810/1‑151 (MQ=255)                                                                                caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGGGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                >  2:80440/1‑151 (MQ=255)                                                                                    aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAac                                                             >  1:43086/1‑150 (MQ=255)                                                                                            caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCg                                                    >  2:267753/1‑151 (MQ=255)                                                                                             aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc                                                   >  1:529451/1‑151 (MQ=255)                                                                                                aCAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCa                                                  >  1:193531/1‑149 (MQ=255)                                                                                                  aaaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                              <  2:339066/151‑1 (MQ=255)                                                                                                      aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                           <  2:502873/150‑1 (MQ=255)                                                                                                         cGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                       >  1:650595/1‑151 (MQ=255)                                                                                                             aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg                                                                                         >  1:706846/1‑97 (MQ=255)                                                                                                             aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg                                                                                         <  2:706803/97‑1 (MQ=255)                                                                                                             aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                   >  2:122705/1‑151 (MQ=255)                                                                                                                   taCAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTc                              >  1:593459/1‑150 (MQ=255)                                                                                                                   taCAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTc                              >  1:124736/1‑150 (MQ=255)                                                                                                                             aaaaCTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAcc                   >  2:755998/1‑151 (MQ=255)                                                                                                                                 cTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGa                >  1:499674/1‑150 (MQ=255)                                                                                                                                 cTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGa                >  1:388880/1‑150 (MQ=255)                                                                                                                                   gCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAAt              <  2:136536/150‑1 (MQ=255)                                                                                                                                        aaaaTACTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGc         >  2:132990/1‑150 (MQ=255)                                                                                                                                          aaTAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc      <  2:248165/151‑1 (MQ=255)                                                                                                                                              gTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa  <  1:648371/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCA  >  NC_000913/1808647‑1808938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑