Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,399	A→G	N119S (AAT→AGT)	mutT →	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,399	0	A	G	100.0%	146.9 / NA	38	N119S (AAT→AGT)	mutT	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/15);  total (23/15)

ACTGGAATATGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTT  >  NC_000913/111253‑111544                                                                                                                                                   |                                                                                                                                                  aCTGGAATATGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGa                                                                                                                                                >  2:586850/1‑150 (MQ=255)  cTGGAATATGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAAc                                                                                                                                              >  2:624749/1‑151 (MQ=255)       atatGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCgg                                                                                                                                           >  2:609830/1‑149 (MQ=255)         atGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAAt                                                                                                                                       >  1:190480/1‑151 (MQ=255)           gAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAAtt                                                                                                                                      <  1:173104/150‑1 (MQ=255)                cccGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGaa                                                                                                                                 <  2:324921/150‑1 (MQ=255)                 ccGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGc                                                                                                                               >  2:380442/1‑151 (MQ=255)                  cGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCt                                                                                                                              >  2:622580/1‑151 (MQ=255)                           atatAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCt                                                                                                                     <  2:472960/151‑1 (MQ=255)                                acacTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAgg                                                                                                                <  1:45711/151‑1 (MQ=255)                                 cacTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGt                                                                                                               >  1:281710/1‑151 (MQ=255)                                    tgtgGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTc                                                                                                              >  2:8136/1‑149 (MQ=255)                                     gtgGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTc                                                                                                              >  2:600946/1‑148 (MQ=255)                                       ggTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATa                                                                                                         <  1:533997/151‑1 (MQ=255)                                                    cGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTcgcc                                                                                            >  1:733965/1‑151 (MQ=255)                                                                                  agaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATg                                                              >  1:169647/1‑151 (MQ=255)                                                                                   gaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGc                                                             >  1:587861/1‑151 (MQ=255)                                                                                     agGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGa                                                           <  1:275242/151‑1 (MQ=255)                                                                                      gggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGAc                                                          <  2:206180/151‑1 (MQ=255)                                                                                                   aGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCtt                                             <  2:147308/151‑1 (MQ=255)                                                                                                         tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                       >  2:431865/1‑151 (MQ=255)                                                                                                           tCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                     >  2:252580/1‑151 (MQ=255)                                                                                                            cGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                     <  2:16381/150‑1 (MQ=255)                                                                                                             gCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                    >  2:431468/1‑150 (MQ=255)                                                                                                                ggtcggtcTTAATGCCGATGATTTTCCGCCAGCCAGTGCACCGGTAATTGCGAAGCTTAAACGCCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTa                                   >  2:474419/1‑148 (MQ=255)                                                                                                                  tcggtcTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                              >  1:6350/1‑151 (MQ=255)                                                                                                                     gtcTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGAtt                           <  1:748380/151‑1 (MQ=255)                                                                                                                       cTTAATGCCGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTc                         >  1:404646/1‑151 (MQ=255)                                                                                                                              ccGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGGCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTc                   >  2:288421/1‑150 (MQ=255)                                                                                                                               cGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCAt                 <  1:609866/151‑1 (MQ=255)                                                                                                                               cGATGATTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCAt                 >  1:508674/1‑151 (MQ=255)                                                                                                                                gatgatTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCAtt                <  2:281482/151‑1 (MQ=255)                                                                                                                                 atgatTTTCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTg               <  2:269136/151‑1 (MQ=255)                                                                                                                                     ttttCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATa            >  1:394241/1‑150 (MQ=255)                                                                                                                                      tttCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATaa           >  1:686337/1‑150 (MQ=255)                                                                                                                                        tCCGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAAt         <  1:739435/150‑1 (MQ=255)                                                                                                                                          cGCCAGCCAGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTg      <  1:142206/151‑1 (MQ=255)                                                                                                                                              agccaGTGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGtttt  >  1:43079/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACTGGAATATGAATTCCCGGACAGGCATATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTT  >  NC_000913/111253‑111544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑