Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,829	T→C	V299A (GTG→GCG)	ydjN →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,829	0	T	C	100.0%	183.3 / NA	52	V299A (GTG→GCG)	ydjN	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976                                                                                                                                                     |                                                                                                                                                    gCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTgcgc                                                                                                                                                   >  1:197764/1‑151 (MQ=255)     gTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGAc                                                                                                                                               <  1:221034/151‑1 (MQ=255)                   gACATCATCAAACTGGGAAGTTTCGATGACGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTCACGTTTGCCTTTAcc                                                                                                                                  >  2:274315/1‑150 (MQ=255)                           caAACTGGGAAGTTTCGTTGTCGCGTCCTCCCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTCCCAGCCGtt                                                                                                                           <  2:197237/149‑1 (MQ=255)                              aCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGt                                                                                                                      >  1:137854/1‑151 (MQ=255)                               cTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg                                                                                                                     >  1:485631/1‑151 (MQ=255)                                  ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgctg                                                                                                                  >  1:184691/1‑151 (MQ=255)                                        ttCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCt                                                                                                             >  2:328536/1‑150 (MQ=255)                                          cGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATc                                                                                                          <  1:163012/151‑1 (MQ=255)                                                  cgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGa                                                                                                   >  2:570617/1‑150 (MQ=255)                                                   gTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAt                                                                                                 >  1:631448/1‑151 (MQ=255)                                                        aCCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTgg                                                                                             <  1:186781/150‑1 (MQ=255)                                                           tCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAg                                                                                          >  2:95776/1‑150 (MQ=255)                                                               tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGcaca                                                                                      >  2:318848/1‑150 (MQ=255)                                                                 tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTACCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACaaa                                                                                    >  2:364547/1‑150 (MQ=255)                                                                     gATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgt                                                                               <  2:521856/151‑1 (MQ=255)                                                                     gATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgt                                                                               >  2:604769/1‑151 (MQ=255)                                                                       ttATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgtcg                                                                             >  1:399363/1‑151 (MQ=255)                                                                         aTGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCgtcgtc                                                                           <  2:453035/151‑1 (MQ=255)                                                                              tGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGc                                                                      >  1:463052/1‑151 (MQ=255)                                                                                cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGATGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGACGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACCAACGCGTCGTCTGGGCGt                                                                    >  1:653366/1‑151 (MQ=255)                                                                                  gTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGtt                                                                   >  2:460590/1‑150 (MQ=255)                                                                                       tGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTg                                                               <  1:758748/149‑1 (MQ=255)                                                                                          cATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATcc                                                          <  1:529410/151‑1 (MQ=255)                                                                                           aTTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCa                                                         <  1:281909/151‑1 (MQ=255)                                                                                               tgctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGc                                                     >  1:489945/1‑151 (MQ=255)                                                                                                gctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATc                                                       <  2:648215/148‑1 (MQ=255)                                                                                                 ctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGcc                                                    >  1:725217/1‑150 (MQ=255)                                                                                                 ctgGGCATTAATGGCGTGAGTCCGCTGAAGGACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGcc                                                    >  2:133654/1‑150 (MQ=255)                                                                                                   gggCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAg                                                  <  2:383940/150‑1 (MQ=255)                                                                                                     gCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGttt                                               <  2:139122/151‑1 (MQ=255)                                                                                                      cATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTc                                              >  1:144197/1‑151 (MQ=255)                                                                                                          aaTGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcgcc                                           <  1:661453/150‑1 (MQ=255)                                                                                                           aTGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcgccgc                                         >  2:691193/1‑151 (MQ=255)                                                                                                             ggCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCct                                       >  1:719385/1‑151 (MQ=255)                                                                                                                gTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCtt                                    >  1:34878/1‑151 (MQ=255)                                                                                                                 tGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGTTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCtt                                    <  2:597986/150‑1 (MQ=255)                                                                                                                  gAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTc                                  <  1:10324/151‑1 (MQ=255)                                                                                                                      ccGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGt                               <  1:92567/150‑1 (MQ=255)                                                                                                                           gAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCaa                           <  2:625906/149‑1 (MQ=255)                                                                                                                              gTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAtt                      <  1:513344/151‑1 (MQ=255)                                                                                                                              gTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAtt                      >  1:470717/1‑151 (MQ=255)                                                                                                                                aCTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTgg                    <  1:62899/151‑1 (MQ=255)                                                                                                                                aCTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTgg                    >  1:545201/1‑151 (MQ=255)                                                                                                                                    ccGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                <  1:647243/151‑1 (MQ=255)                                                                                                                                       tAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc             >  2:587852/1‑151 (MQ=255)                                                                                                                                          ggTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGc          <  1:523840/151‑1 (MQ=255)                                                                                                                                           gTATGGCATGCCCCGACGTTTGGCTTTACCAGCCGTTCCAGTGCTGCTTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGACCGGCt         <  2:498531/151‑1 (MQ=255)                                                                                                                                               ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCt         <  2:365118/147‑1 (MQ=255)                                                                                                                                               ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgcgc     >  1:106332/1‑151 (MQ=255)                                                                                                                                               ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgcgc     <  1:570648/151‑1 (MQ=255)                                                                                                                                                gCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGcc    <  1:268712/151‑1 (MQ=255)                                                                                                                                                  cTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCgg  >  1:124740/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑