Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,238	T→C	V66V (GTA→GTG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,238	0	T	C	100.0%	191.6 / NA	50	V66V (GTA→GTG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/34);  total (16/34)

GTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGT  >  NC_000913/1907092‑1907375                                                                                                                                                   |                                                                                                                                          gtgtAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGt                                                                                                                                       <  1:345260/151‑1 (MQ=255)      aCGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACa                                                                                                                                  <  2:349516/151‑1 (MQ=255)       cgcgAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAg                                                                                                                                 <  2:223523/151‑1 (MQ=255)          gAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTg                                                                                                                              <  2:533166/151‑1 (MQ=255)                   ggcggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCt                                                                                                                      <  2:119004/150‑1 (MQ=255)                     cggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTc                                                                                                                   <  1:37358/151‑1 (MQ=255)                     cggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTc                                                                                                                   <  2:17856/151‑1 (MQ=255)                       ggCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTg                                                                                                                 <  1:631481/151‑1 (MQ=255)                        gCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTgg                                                                                                                >  1:83691/1‑151 (MQ=255)                          aaaTATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGcc                                                                                                              <  1:179716/151‑1 (MQ=255)                            atatCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGt                                                                                                            <  1:429267/151‑1 (MQ=255)                                ccGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        >  1:352748/1‑151 (MQ=255)                                ccGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        <  2:127334/151‑1 (MQ=255)                                 cGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        >  1:87803/1‑150 (MQ=255)                                  gAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGaa                                                                                                      <  2:48069/151‑1 (MQ=255)                                     cGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAttt                                                                                                   <  1:147172/151‑1 (MQ=255)                                     cGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAttt                                                                                                   <  1:702007/151‑1 (MQ=255)                                           gAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACt                                                                                             >  1:568306/1‑151 (MQ=255)                                               gAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAAc                                                                                         <  2:661717/151‑1 (MQ=255)                                                       aGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGAc                                                                                 <  2:52845/151‑1 (MQ=255)                                                            aGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCa                                                                            <  1:215802/151‑1 (MQ=255)                                                                 aaaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  2:493768/151‑1 (MQ=255)                                                                 aaaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  2:141513/151‑1 (MQ=255)                                                                  aaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                         >  1:518907/1‑148 (MQ=255)                                                                    gCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGCTTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCaga                                                                      >  2:344852/1‑149 (MQ=255)                                                                      cTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       >  2:544730/1‑146 (MQ=255)                                                                      cTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  1:544758/146‑1 (MQ=255)                                                                          cATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                              <  2:94785/151‑1 (MQ=255)                                                                             tCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                            <  1:389097/150‑1 (MQ=255)                                                                               gacgaGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                         <  2:504525/151‑1 (MQ=255)                                                                                acgaGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt                                                        <  1:904/151‑1 (MQ=255)                                                                                     gCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCt                                                   >  1:246297/1‑151 (MQ=255)                                                                                      cTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                  >  2:622186/1‑151 (MQ=255)                                                                                       tttATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                  >  2:712263/1‑150 (MQ=255)                                                                                          ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa                                              <  1:544231/151‑1 (MQ=255)                                                                                           taGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa                                              <  2:732407/150‑1 (MQ=255)                                                                                             gCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                           <  2:518883/151‑1 (MQ=255)                                                                                                agaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                        <  1:416651/151‑1 (MQ=255)                                                                                                     gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                   >  2:51242/1‑151 (MQ=255)                                                                                                       aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtgt                                  >  2:671963/1‑150 (MQ=255)                                                                                                       aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                 <  1:336369/151‑1 (MQ=255)                                                                                                         cGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                               <  1:104139/151‑1 (MQ=255)                                                                                                            aTTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAac                            >  2:344153/1‑151 (MQ=255)                                                                                                              tCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                           <  1:600956/150‑1 (MQ=255)                                                                                                                    ttCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCttt                    >  1:703247/1‑151 (MQ=255)                                                                                                                    ttCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                     >  1:600566/1‑150 (MQ=255)                                                                                                                               ggATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCTATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg         <  2:757590/151‑1 (MQ=255)                                                                                                                                    cGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCTGa    <  2:136873/151‑1 (MQ=255)                                                                                                                                      aTCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt  <  2:87802/151‑1 (MQ=255)                                                                                                                                       tCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt  >  2:577612/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                          GTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGT  >  NC_000913/1907092‑1907375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑