Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,951,184	G→A	R118H (CGC→CAC)	yecD →	isochorismatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,951,184	0	G	A	100.0%	185.6 / NA	46	R118H (CGC→CAC)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/16);  total (30/16)

CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACA  >  NC_000913/1951037‑1951324                                                                                                                                                    |                                                                                                                                             cGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGccaccg                                                                                                                                           <  2:606659/151‑1 (MQ=255)  ggTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCACACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGccaccg                                                                                                                                           >  2:260789/1‑150 (MQ=255)  ggTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGa                                                                                                                                          <  1:547662/151‑1 (MQ=255)           gcCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  2:93209/1‑151 (MQ=255)           gcCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  1:160787/1‑151 (MQ=255)            cccTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  1:204932/1‑150 (MQ=255)                cACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAAt                                                                                                                            <  1:2103/151‑1 (MQ=255)                  ccGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATa                                                                                                                           <  1:431518/150‑1 (MQ=255)                  ccGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAg                                                                                                                          <  1:470267/151‑1 (MQ=255)                   cGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgt                                                                                                                         >  1:163754/1‑151 (MQ=255)                    ggCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtg                                                                                                                        >  1:319576/1‑151 (MQ=255)                     gCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtgt                                                                                                                       >  2:523526/1‑151 (MQ=255)                      cTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGtt                                                                                                                      >  2:395194/1‑151 (MQ=255)                        aaaGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTa                                                                                                                     <  1:120530/150‑1 (MQ=255)                         aaGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTAtg                                                                                                                   >  1:287828/1‑151 (MQ=255)                          aGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTAtgt                                                                                                                  >  1:536795/1‑151 (MQ=255)                            tgtTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTgg                                                                                                                >  1:526018/1‑151 (MQ=255)                            tgtTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGGGTTATGTgg                                                                                                                >  2:105475/1‑151 (MQ=255)                              ttGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTggg                                                                                                               >  1:41712/1‑150 (MQ=255)                                 cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtct                                                                                                           >  2:302401/1‑151 (MQ=255)                                 cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtct                                                                                                           <  2:593200/151‑1 (MQ=255)                                 cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtc                                                                                                            >  2:446604/1‑150 (MQ=255)                                        aTTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCa                                                                                                     >  2:269306/1‑150 (MQ=255)                                                 aaCATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGgtgt                                                                                           >  2:590052/1‑151 (MQ=255)                                                      ccTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGaa                                                                                       >  1:732896/1‑150 (MQ=255)                                                      ccTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAAt                                                                                      >  2:751032/1‑151 (MQ=255)                                                        tgctgcATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATcc                                                                                    >  1:200337/1‑151 (MQ=255)                                                            gctttAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccg                                                                                >  2:514541/4‑151 (MQ=255)                                                            gcATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAc                                                                                  <  2:600983/149‑1 (MQ=255)                                                              aTTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccgcc                                                                              >  2:615693/1‑151 (MQ=255)                                                                  ggTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCa                                                                          <  1:293478/151‑1 (MQ=255)                                                                  ggTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCa                                                                          <  1:495953/151‑1 (MQ=255)                                                                         ccGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTg                                                                   <  2:4431/151‑1 (MQ=255)                                                                          cGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTgg                                                                  >  1:106969/1‑151 (MQ=255)                                                                          cGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTgg                                                                  >  2:115296/1‑151 (MQ=255)                                                                            aCAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGa                                                                <  2:536768/151‑1 (MQ=255)                                                                                    atCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCt                                                        <  2:664335/151‑1 (MQ=255)                                                                                     tCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCtt                                                       >  1:516910/1‑151 (MQ=255)                                                                                                   cGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTg                                         >  1:427367/1‑151 (MQ=255)                                                                                                            ggTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGAc                                 >  2:760540/1‑150 (MQ=255)                                                                                                               gCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCt                             >  1:284738/1‑151 (MQ=255)                                                                                                                 gTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGt                           >  1:619371/1‑151 (MQ=255)                                                                                                                     tacggtacggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTg                       >  2:267479/1‑151 (MQ=255)                                                                                                                           acggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCa                 <  1:546681/151‑1 (MQ=255)                                                                                                                                tCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGcc            >  2:542256/1‑151 (MQ=255)                                                                                                                                   ggAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAgcgc             <  1:745485/147‑1 (MQ=255)                                                                                                                                     aGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGagca       <  1:173504/151‑1 (MQ=255)                                                                                                                                          cAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGcaca  <  1:756619/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACA  >  NC_000913/1951037‑1951324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑