Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,168,019 G→A V96V (GTC→GTT yegR ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,168,0190GA100.0% 53.6 / NA 14V96V (GTC→GTTyegRuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/12);  total (2/12)

ATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAGCTTCGGC  >  NC_000913/2167877‑2168159
                                                                                                                                              |                                                                                                                                            
aTAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATg                                                                                                                                      >  2:111927/1‑151 (MQ=255)
       cctcctTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCt                                                                                                                               <  1:672915/151‑1 (MQ=255)
                    aCGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCt                                                                                                                  <  1:528510/151‑1 (MQ=255)
                          ccTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCt                                                                                                            <  2:226878/151‑1 (MQ=255)
                              tCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTc                                                                                                                       >  1:639263/1‑136 (MQ=255)
                              tCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTc                                                                                                                       <  2:639224/136‑1 (MQ=255)
                                      cGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGc                                                                                                 <  2:221771/150‑1 (MQ=255)
                                                                          ccAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGcat                                                            <  1:186166/151‑1 (MQ=255)
                                                                                                         aaTATTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGatat                             <  1:326484/151‑1 (MQ=255)
                                                                                                          atatTCTCACTCTCCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATAtt                            <  2:353259/151‑1 (MQ=255)
                                                                                                                     ctcCACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTg                   <  2:9768/149‑1 (MQ=255)
                                                                                                                       ccACATTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTgg                  <  1:98466/148‑1 (MQ=255)
                                                                                                                         acaTTTGAATGTCAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTaaa             <  2:477999/151‑1 (MQ=255)
                                                                                                                                     cAGACGAGCAACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAgcttcggc  <  1:111929/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
ATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAGCTTCGGC  >  NC_000913/2167877‑2168159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: