Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,366	T→C	K208K (AAA→AAG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,366	0	T	C	100.0%	212.4 / NA	55	K208K (AAA→AAG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/31);  total (24/31)

AGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGA  >  NC_000913/2221221‑2221508                                                                                                                                                  |                                                                                                                                               aGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGAc                                                                                                                                           >  1:383910/1‑151 (MQ=255)     cAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCt                                                                                                                                        <  1:665121/150‑1 (MQ=255)           caccaTCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGtcatc                                                                                                                                 <  2:708426/151‑1 (MQ=255)             ccaTCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCAc                                                                                                                               >  1:631852/1‑151 (MQ=255)              caTCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCAcc                                                                                                                              >  2:116797/1‑151 (MQ=255)                    cgcgCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCTTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGtgg                                                                                                                        >  1:664304/1‑149 (MQ=255)                       gccgcTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCg                                                                                                                      >  1:567577/1‑150 (MQ=255)                         cgcTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGa                                                                                                                     <  2:261037/149‑1 (MQ=255)                             gcctgcGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGc                                                                                                                <  1:113325/150‑1 (MQ=255)                                 gcGTCCAGCCCCGCGTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTGCCGCCTTCTGCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCggg                                                                                                            <  2:165343/150‑1 (MQ=255)                                  cGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCt                                                                                                          >  1:434230/1‑151 (MQ=255)                                  cGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCt                                                                                                          >  1:492855/1‑151 (MQ=255)                                          cccGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCt                                                                                                  <  1:302055/151‑1 (MQ=255)                                                ttGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATc                                                                                             <  1:668737/150‑1 (MQ=255)                                                 tGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATc                                                                                             <  2:526490/149‑1 (MQ=255)                                                 tGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCg                                                                                            <  2:391870/150‑1 (MQ=255)                                                 tGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCg                                                                                            <  1:661050/150‑1 (MQ=255)                                                  gTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCt                                                                                          <  2:454880/151‑1 (MQ=255)                                                           cATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATg                                                                                 <  1:654388/151‑1 (MQ=255)                                                              ataATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGtt                                                                              >  1:758412/1‑151 (MQ=255)                                                                aaTCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGttt                                                                             <  2:444723/150‑1 (MQ=255)                                                                 aTCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTa                                                                           <  2:82134/151‑1 (MQ=255)                                                                    aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTAccc                                                                        >  1:334165/1‑151 (MQ=255)                                                                    aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTAccc                                                                        >  1:9243/1‑151 (MQ=255)                                                                          cAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAttgtt                                                                  <  1:219608/151‑1 (MQ=255)                                                                                cTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTg                                                            >  1:540313/1‑151 (MQ=255)                                                                                   cGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGCTTTACCCATTGTTGAGGTg                                                            >  1:371505/1‑148 (MQ=255)                                                                                       cTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACg                                                     >  2:303967/1‑151 (MQ=255)                                                                                         cATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACg                                                     <  1:612107/149‑1 (MQ=255)                                                                                         cATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACg                                                     <  1:507426/149‑1 (MQ=255)                                                                                               gACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCg                                             <  1:135412/151‑1 (MQ=255)                                                                                                  ggTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGcc                                           >  1:254861/1‑150 (MQ=255)                                                                                                  ggTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCa                                          <  1:458071/151‑1 (MQ=255)                                                                                                    tgttgtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCaa                                         >  2:309303/1‑150 (MQ=255)                                                                                                       tgtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAAc                                      <  1:518378/150‑1 (MQ=255)                                                                                                        gtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCt                                    <  2:751036/151‑1 (MQ=255)                                                                                                        gtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCt                                    <  1:346767/151‑1 (MQ=255)                                                                                                          aCTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTc                                  <  1:138863/151‑1 (MQ=255)                                                                                                          aCTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTc                                  <  1:567833/151‑1 (MQ=255)                                                                                                                tcccGCCTTCTTCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGa                               <  2:441344/146‑1 (MQ=255)                                                                                                                 aCCGCCTTCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGc                            <  1:264676/150‑1 (MQ=255)                                                                                                                        tCTACCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTcccc                    >  2:210322/1‑151 (MQ=255)                                                                                                                           aCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAgc                 <  1:38643/151‑1 (MQ=255)                                                                                                                           aCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAgc                 >  1:544555/1‑151 (MQ=255)                                                                                                                           aCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAg                  <  1:42909/150‑1 (MQ=255)                                                                                                                           aCCGCGCCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGCCCCCAgc                 >  1:350016/1‑151 (MQ=255)                                                                                                                                gcCGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCgg             <  2:180079/150‑1 (MQ=255)                                                                                                                                 ccGTATGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGAt           <  2:612608/151‑1 (MQ=255)                                                                                                                                     aTGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGCGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcg       >  1:758988/1‑151 (MQ=255)                                                                                                                                     aTGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcg       >  2:70671/1‑151 (MQ=255)                                                                                                                                     aTGCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGATTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCTCCAGCGCGGATcgc        >  2:484749/1‑150 (MQ=255)                                                                                                                                       gCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcgcg     >  2:267074/1‑151 (MQ=255)                                                                                                                                       gCGGCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcgcg     >  1:616089/1‑151 (MQ=255)                                                                                                                                         ggCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcgcg     >  1:198212/1‑149 (MQ=255)                                                                                                                                          gCAAAGTGCTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCgaga  <  1:593611/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               AGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGA  >  NC_000913/2221221‑2221508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑