Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,346,802	G→A	A646A (GCG→GCA)	nrdA →	ribonucleoside‑diphosphate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,346,802	0	G	A	100.0%	225.1 / NA	57	A646A (GCG→GCA)	nrdA	ribonucleoside‑diphosphate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/26);  total (31/26)

ACTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCC  >  NC_000913/2346657‑2346949                                                                                                                                                  |                                                                                                                                                    aCTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGaa                                                                                                                                                <  1:421943/151‑1 (MQ=255) aCTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGa                                                                                                                                                 >  1:24893/1‑150 (MQ=255)      gcaGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACg                                                                                                                                           >  2:526655/3‑151 (MQ=255)              gCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTg                                                                                                                                   >  1:236704/1‑151 (MQ=255)              gCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTg                                                                                                                                   <  2:157247/151‑1 (MQ=255)                gTGAGTCAATCAAACCGCACGGTCTCCGTAACACCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTACCGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTg                                                                                                                                   <  2:505822/149‑1 (MQ=255)                  gAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGcc                                                                                                                               >  2:509698/1‑151 (MQ=255)                   aGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGcc                                                                                                                               >  1:127052/1‑150 (MQ=255)                    gTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAg                                                                                                                             <  2:718261/151‑1 (MQ=255)                     tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAgg                                                                                                                            >  1:320067/1‑151 (MQ=255)                           aaaaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGc                                                                                                                      >  2:591802/1‑151 (MQ=255)                           aaaaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGc                                                                                                                      <  2:477916/151‑1 (MQ=255)                                        tGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGc                                                                                                          >  2:34047/1‑150 (MQ=255)                                           gTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCt                                                                                                      <  1:638007/151‑1 (MQ=255)                                            tAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTg                                                                                                     >  2:648905/1‑151 (MQ=255)                                             aaCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGc                                                                                                    >  2:213120/1‑151 (MQ=255)                                               cTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCac                                                                                                  >  2:243077/1‑151 (MQ=255)                                                    cGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGc                                                                                             >  2:553509/1‑151 (MQ=255)                                                        ttCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTAt                                                                                         >  2:740585/1‑151 (MQ=255)                                                         tCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTAt                                                                                         <  1:488622/150‑1 (MQ=255)                                                         tCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTAt                                                                                         <  1:54238/150‑1 (MQ=255)                                                                  aTGCCTTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCtgt                                                                               <  2:143336/151‑1 (MQ=255)                                                                   tGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCtgt                                                                               <  2:348247/150‑1 (MQ=255)                                                                     ccGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTggg                                                                            <  1:491788/151‑1 (MQ=255)                                                                       gTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGa                                                                           >  2:85475/1‑150 (MQ=255)                                                                         ccGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGaaa                                                                         <  2:507830/150‑1 (MQ=255)                                                                         ccGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAAt                                                                        >  1:50729/1‑151 (MQ=255)                                                                               cttcttCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCggg                                                                  >  2:44555/1‑151 (MQ=255)                                                                                 tcttcGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCggg                                                                  <  1:744059/149‑1 (MQ=255)                                                                                    tcGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTa                                                                <  1:546167/148‑1 (MQ=255)                                                                                       cAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGAt                                                           <  2:619804/150‑1 (MQ=255)                                                                                        aGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATgg                                                         <  2:99722/151‑1 (MQ=255)                                                                                        aGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATgg                                                         >  1:601438/1‑151 (MQ=255)                                                                                          aTCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGCAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGtt                                                       >  2:83471/1‑151 (MQ=255)                                                                                              ctAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGt                                                        <  1:412032/146‑1 (MQ=255)                                                                                              ctAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCt                                                   >  2:202447/1‑151 (MQ=255)                                                                                                 aCGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGc                                                 >  2:440539/1‑150 (MQ=255)                                                                                                  cGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCa                                                >  2:433608/1‑150 (MQ=255)                                                                                                   gCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAAc                                              <  1:40891/151‑1 (MQ=255)                                                                                                    ccACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACt                                             >  2:453860/1‑151 (MQ=255)                                                                                                    ccACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACt                                             <  1:549167/151‑1 (MQ=255)                                                                                                      aCTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACtgg                                           >  2:122031/1‑151 (MQ=255)                                                                                                      aCTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACtgg                                           >  2:9944/1‑151 (MQ=255)                                                                                                       cTAACGGAATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACtgg                                           >  2:609281/1‑150 (MQ=255)                                                                                                            ggTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGt                                      >  2:457770/1‑150 (MQ=255)                                                                                                               aTTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCa                                  <  1:31118/151‑1 (MQ=255)                                                                                                                 tGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATg                                >  1:106640/1‑151 (MQ=255)                                                                                                                 tGAACCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATg                                >  2:151370/1‑151 (MQ=255)                                                                                                                   aaCCGCCGCGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGc                               >  2:324107/1‑150 (MQ=255)                                                                                                                       gccgcGCGGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGaaa                          <  1:108139/151‑1 (MQ=255)                                                                                                                            gcgGTTACGTCAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTAt                     <  1:674041/151‑1 (MQ=255)                                                                                                                                      cAGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCg           >  1:163986/1‑151 (MQ=255)                                                                                                                                       aGCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCg           >  2:86370/1‑150 (MQ=255)                                                                                                                                        gCATCAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGAt         <  1:3894/151‑1 (MQ=255)                                                                                                                                            cAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGAtctc      <  1:69330/150‑1 (MQ=255)                                                                                                                                            cAAAGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATctct     <  1:61611/151‑1 (MQ=255)                                                                                                                                               aGCATCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGcc  <  1:604642/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCCGCGCGGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCC  >  NC_000913/2346657‑2346949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑