Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,366,486	G→A	A187T (GCT→ACT)	arnB →	uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,366,486	0	G	A	100.0%	164.1 / NA	42	A187T (GCT→ACT)	arnB	uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

CCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACC  >  NC_000913/2366343‑2366622                                                                                                                                                |                                                                                                                                         ccATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAgg                                                                                                                                   <  1:274475/151‑1 (MQ=255)   aTTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTg                                                                                                                                 <  1:75736/151‑1 (MQ=255)     tCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTgg                                                                                                                                <  1:257322/150‑1 (MQ=255)        cgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTg                                                                                                                            >  1:516684/1‑151 (MQ=255)             ttGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGt                                                                                                                       >  1:764521/1‑151 (MQ=255)                 cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACt                                                                                                                   <  2:83332/151‑1 (MQ=255)                  gAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTA‑TACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGa                                                                                                                 <  1:731383/151‑1 (MQ=255)                    aCGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGAt                                                                                                                <  1:640217/151‑1 (MQ=255)                      gTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATaa                                                                                                              <  2:375549/151‑1 (MQ=255)                            gCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGaaa                                                                                                         >  2:239046/1‑150 (MQ=255)                                cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                    >  1:687422/1‑151 (MQ=255)                                cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                    >  1:194960/1‑151 (MQ=255)                                 gCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTg                                                                                                   <  1:756453/151‑1 (MQ=255)                                 gCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTg                                                                                                   <  1:135943/151‑1 (MQ=255)                                   aGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGcc                                                                                                 <  1:661058/151‑1 (MQ=255)                                            aGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTa                                                                                        <  2:765188/151‑1 (MQ=255)                                              aTGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACg                                                                                      <  2:764474/151‑1 (MQ=255)                                               tgctgcCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACgg                                                                                     >  2:48983/1‑151 (MQ=255)                                                gctgcCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACgg                                                                                     >  1:463623/1‑150 (MQ=255)                                                         gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGaaa                                                                            <  1:736684/150‑1 (MQ=255)                                                         gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAAt                                                                           >  2:197978/1‑151 (MQ=255)                                                                  aCGTATTCCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGt                                                                   <  2:94236/150‑1 (MQ=255)                                                                      aTTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTGCGGATGCTGAAATTTCACGGTCTggg                                                              >  2:417359/1‑151 (MQ=255)                                                                           aaaGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCg                                                         >  2:394386/1‑151 (MQ=255)                                                                            aaGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGa                                                        <  1:399057/151‑1 (MQ=255)                                                                                gCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGcc                                                    >  2:362861/1‑151 (MQ=255)                                                                                  gACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCt                                                   >  1:308820/1‑150 (MQ=255)                                                                                      tatTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgac                                              >  1:680113/1‑151 (MQ=255)                                                                                         tGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgac                                              >  2:407635/1‑148 (MQ=255)                                                                                                       ccGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGcc                              <  2:305275/150‑1 (MQ=255)                                                                                                                tttcatttcaTGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGc                     >  1:472859/1‑150 (MQ=255)                                                                                                                   catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAgg                  <  1:239055/150‑1 (MQ=255)                                                                                                                   catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGc                 <  2:82519/151‑1 (MQ=255)                                                                                                                    atttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                >  1:550205/1‑151 (MQ=255)                                                                                                                       tcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTg                           >  1:715154/1‑137 (MQ=255)                                                                                                                       tcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTg                           <  2:715110/137‑1 (MQ=255)                                                                                                                        catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGaa             <  1:97414/150‑1 (MQ=255)                                                                                                                        catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAg            <  2:266403/151‑1 (MQ=255)                                                                                                                                tAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAac     >  1:635475/1‑150 (MQ=255)                                                                                                                                 aaaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCt         >  2:156257/1‑145 (MQ=255)                                                                                                                                 aaaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCt         <  1:156264/145‑1 (MQ=255)                                                                                                                                  aaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACAcc  >  2:588288/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                         CCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACC  >  NC_000913/2366343‑2366622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑