Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,438,860	A→G	D304G (GAC→GGC)	flk →	putative flagella assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,438,860	0	A	G	100.0%	190.7 / NA	51	D304G (GAC→GGC)	flk	putative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/24);  total (27/24)

CCAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAA  >  NC_000913/2438714‑2439006                                                                                                                                                   |                                                                                                                                                   ccAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGTGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACg                                                                                                                                                <  2:450596/151‑1 (MQ=255)   aGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGtt                                                                                                                                              <  1:449918/151‑1 (MQ=255)     cAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGc                                                                                                                                            <  2:467556/151‑1 (MQ=255)       aaaaTATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTTATCCTTTTTCACCGATGATGGGCACGTTTCa                                                                                                                                           >  2:243815/1‑150 (MQ=255)         aaTATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCaaa                                                                                                                                         >  1:672075/1‑150 (MQ=255)          atatGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAat                                                                                                                                       <  1:751261/151‑1 (MQ=255)                  tttCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTTCACCGATGATGGGCACGTTGCAAAATATGGCaa                                                                                                                                >  2:160719/1‑150 (MQ=255)                   ttCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAAGGATTGATACGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAAc                                                                                                                               >  2:220424/1‑150 (MQ=255)                     cTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGAAGGGCACGTTGCAAAAAATGGCAAcgc                                                                                                                             >  2:687305/1‑150 (MQ=255)                       gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTc                                                                                                                          <  1:173852/151‑1 (MQ=255)                                 cGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCtgt                                                                                                                 <  2:714956/150‑1 (MQ=255)                                   cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                               >  2:599293/1‑150 (MQ=255)                                       cttctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGGTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACt                                                                                                          >  2:190941/1‑151 (MQ=255)                                         tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                         <  2:693203/150‑1 (MQ=255)                                         tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGt                                                                                                        >  1:623813/1‑151 (MQ=255)                                               cAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                  <  1:374696/151‑1 (MQ=255)                                                aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGTTATGCAACCTCTGCTTAATCCTTTTCCACCGATGAAGGGCACGTTGCAAAATATGGCAAAGCGTCCCGCGCCGTGGATACTGTTAGTc                                                                                                   >  2:592291/1‑149 (MQ=255)                                                aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATTCAACCGCCGCTTAAACCTTTTCCACCGAAGATGGGCACGATGCAAAAAATGGCCACACGGCCCACCCCGTGGATAccttttgt                                                                                                    >  2:76682/1‑141 (MQ=255)                                                 ggCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                <  2:612882/151‑1 (MQ=255)                                                    cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:178929/1‑151 (MQ=255)                                                    cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:225897/1‑151 (MQ=255)                                                    cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGGTAAAGGGATTGAACCGCGTGTTATGCAACCCCTGCTTAAACCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCCACCCGTCCCGCGCTGTGGGTACTGTTAGTCGCGAtt                                                                                             >  1:131968/1‑151 (MQ=255)                                                     gAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAAGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:439285/1‑150 (MQ=255)                                                      aGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTAt                                                                                           <  1:107250/151‑1 (MQ=255)                                                       ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTTCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGGGATTAt                                                                                           >  2:559549/1‑150 (MQ=255)                                                        gATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATc                                                                                          >  1:43529/1‑150 (MQ=255)                                                            ttCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCCAAATATGGCAACGCGTCCCCCGCTGTGGATACTGTTAGTCGCGATTATCCTGAt                                                                                     >  1:586502/1‑151 (MQ=255)                                                                tAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATg                                                                                    <  2:704865/148‑1 (MQ=255)                                                                 aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                 <  2:614039/150‑1 (MQ=255)                                                                  aTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGt                                                                               <  2:542808/151‑1 (MQ=255)                                                                       cTTTATCAACGGCGGGTTAAAGGGATTGATTCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctgg                                                                           >  1:458249/1‑150 (MQ=255)                                                                         ttATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctg                                                                        >  2:372457/1‑151 (MQ=255)                                                                          tATCAACGGCGGGGTAAAGGGATTGATCCGGGTGGTATGCAAGCGCTTCTTAATCCTTTTCCACCGATTAGGGGCAAGTTGCAAAATATGGCAACGCGGCCCGCGGTTTGGATACTTGTTGGCGCGAATATCCTGATGCTGGTcttg                                                                           >  1:233711/1‑145 (MQ=255)                                                                             cAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTc                                                                    <  1:323058/151‑1 (MQ=255)                                                                                      gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcccc                                                           <  1:639664/151‑1 (MQ=255)                                                                                        tAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcg                                                          >  2:215280/1‑150 (MQ=255)                                                                                             ggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGaaa                                                    <  2:220660/151‑1 (MQ=255)                                                                                                   aTCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                              <  2:485363/151‑1 (MQ=255)                                                                                                   aTCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                              <  1:483053/151‑1 (MQ=255)                                                                                                     ccGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGt                                             <  1:635434/150‑1 (MQ=255)                                                                                                             tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGCTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                     >  1:550005/1‑150 (MQ=255)                                                                                                              aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACa                                    <  2:581421/150‑1 (MQ=255)                                                                                                                     cGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAcc                            >  1:710724/1‑151 (MQ=255)                                                                                                                          ctTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGc                        >  2:509045/1‑150 (MQ=255)                                                                                                                          ctTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGc                        >  1:728914/1‑150 (MQ=255)                                                                                                                           ttAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAc                      >  2:206417/1‑151 (MQ=255)                                                                                                                                ccTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCa                  <  2:703312/150‑1 (MQ=255)                                                                                                                                          cGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGcccc       <  1:644330/151‑1 (MQ=255)                                                                                                                                           gatgatGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCg      >  2:419068/1‑151 (MQ=255)                                                                                                                                             tgatgGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCgg     <  2:638093/150‑1 (MQ=255)                                                                                                                                               atgGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCaa  <  1:575511/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAA  >  NC_000913/2438714‑2439006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑